Jerusalem, Oct. 2, 2011 A factor that is crucial for the proper positioning of genes in the cell nucleus has been discovered by a team of researchers from the Alexander Silberman Institute of Life Sciences at the Hebrew University of Jerusalem and the Friedrich Miescher Institute for Biomedical Research in Basel, Switzerland.
The researchers found that the lamin filamentous network is an essential element in this proper positioning, the lack of which can cause specific diseases. Lamin proteins make filaments that are located mainly at the periphery of the cell nucleus, which stores and transcribes genetic material in all living matter. The lamins maintain the nuclear shape and help organize chromosomes.
Mutations in the genes that encode lamin proteins cause 14 different diseases in man, collectively termed laminopathies. These include early aging diseases and diseases that affect peripheral neurons, heart, skin, bones and muscles.
One of the muscle diseases caused by dominant mutations in the gene encoding lamin A is Emery-Dreifuss muscular dystrophy (AD-EDMD). It is characterized by weakening in certain skeletal muscles and early contractures at the neck, elbows and Achilles tendons, as well as cardiac conduction defects. How these mutations lead to the disease was largely unknown.
By manipulating the lamin gene in the worm Caenorhabditis elegans, Prof. Yosef Gruenbaum of the Hebrew University of Jerusalem and his students Anna Mattout and Erin Bank, together with Prof. Susan Gasser of the Friedrich Miescher Institute for Biomedical Research and her students Brietta Pike, Benjamin Towbin, Adriana Gonzalez and Peter Meister were able to show that lamin is necessary for the positioning of regions in the DNA that is mostly inactive (heterochromatin).
They then introduced low levels of a lamin carrying a mutation, which in humans causes AD-EDMD, into the worms and tracked their expression. In the worms
|Contact: Jerry Barach |
The Hebrew University of Jerusalem