Navigation Links
Expanding the range of nature's catalysts for industrial applications
Date:1/30/2014

s properly only when folded into this shape, and typically retains its structure within a narrow range of conditions. If subjected to temperature, pH, or pressure outside these tolerances, the enzyme begins to denature, losing its shape and functionality.

Makhatadze seeks to boost the high-temperature tolerances for a given enzyme by adjusting the electrostatic interactions on the protein surface. In research culminating with the 2009 PNAS paper, Makhatadze developed a computer program allowing researchers to expand the temperature range at which a given enzyme would remain functional by altering the electrical charges on the protein surface.

"Many forces the packing of the core, hydrophobic interactions, hydrogen bonding, salt bridges, disulfide bridges are important for protein stability, and 40 years of research has gone into establishing the rules that govern this process," said Makhatadze. "Our contribution has been on the particular role of the interactions between the charges on the protein surface, and a recognition that they can be manipulated to modulate protein stability."

In the context of industrial processes like paper manufacturing, the expanded functional range could make an enzymatic approach more attractive and economically feasible. The next step, said Makhatadze, and the focus of the NSF grant, is to understand the speed at which proteins fold and unfold, in order to slow their deterioration, and further expand their functional range.

"We've learned to make changes in the stability of the protein. But every protein has a limit; there's nothing you can do to make a protein stable at 500 degrees, for example," said Makhatadze. "So can we somehow make it unfold more slowly by modulating the charge-charge interactions? If you can extend that process, it will function at a high temperature for a longer period of time, and that's beneficial."

Within CBIS, the $1.7 million commitment from the NSF is one o
'/>"/>

Contact: Mary Martialay
martim12@rpi.edu
Rensselaer Polytechnic Institute
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Expanding research communities and collaborations
2. Mobile LIDAR technology expanding rapidly
3. Parasites of Madagascars lemurs expanding with climate change
4. Site-specific, long-term research expanding understanding of climate change
5. Parents seen as critical stakeholders in expanding newborn screening
6. LABS, Inc. Launches Suite of Next-Generation Test Offerings; Focuses on Expanding Complex Biologic Testing Portfolio in 2012
7. Study shows large carnivore numbers and range declining worldwide
8. Control malaria by segmenting sleeping arrangements
9. Wide range of differences, mostly unseen, among humans
10. How chewing gum or a shed hair can let strangers read your Book of Life
11. Immunohistochemistry effectively detects ALK rearrangement
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:9/2/2014)... the Tithonian Konservat-Lagersttte of lithographic limestone is well ... emblematic fossils from that area (for example, Archaeopteryx). ... fossil insects in the French equivalent of these ... representing the oldest known water treader. , Despite ... outcrops, fewer fossils have been obtained from the ...
(Date:9/1/2014)... us fat? An increasing amount of research shows an ... a more sedentary lifestyle. Now, a new Cornell University ... Some TV programs might lead people to eat twice ... you,re watching an action movie while snacking your mouth ... lead author on the new article just published in ...
(Date:9/1/2014)... long held that corals whose calcium-carbonate skeletons ... passive organisms that rely entirely on ocean currents ... oxygen. But now scientists at MIT and the ... found that they are far from passive, engineering ... that greatly enhance their ability to exchange nutrients ...
Breaking Biology News(10 mins):Exceptionally well preserved insect fossils from the Rhône Valley 2Can action movies make you fat? 2Nature's tiny engineers 2Nature's tiny engineers 3
... University, in collaboration with Michigan State University, has identified a ... workers. The scientists unraveled the gene,s inner workings and ... Letters . The gene, which is responsible for leg and ... bees, ability to carry pollen. "The gene Ultrabithorax, ...
... means to increase vegetation and preserve aesthetics in old ... character and biodiversity, researchers are looking to native plant ... are necessary in lightweight green roof design. Xerophytes ... environments with little water--fit well in green roof construction ...
... Paying people to protect their natural environment is a ... out that return on investment, for both people and ... typically stretch on for years. "Short attention-span worlds ... former Michigan State University doctoral student now on faculty ...
Cached Biology News:Research led by Wayne State discovers single gene in bees separating queens from workers 2Three native aromatics indicated for use in Mediterranean extensive green roofs 2To calculate long-term conservation pay off, factor in people 2
(Date:9/2/2014)... HILL and NEW PROVIDENCE, N.J. ... America, Inc. is a corporate sponsor for MassBio,s ... its sister organization, the MassBioEd Foundation. The event will ... Golf Club in Plymouth, Massachusetts . ... biotechnology education in Massachusetts through ...
(Date:9/2/2014)... Iowa (PRWEB) September 02, 2014 Press ... of ways to take advantage of the people and ... the schedule below are available throughout the day. Additionally, ... requests with POET and DSM representatives as well as ... interview requests is appreciated. , Please remember to ...
(Date:9/2/2014)... Philadelphia, PA (PRWEB) September 02, 2014 ... pioneered a new highly efficient, cost effective approach ... U.S. Patent No. 8,820,265 by the U.S. Patent ... proprietary to Opertech. , “Opertech’s taste ... is the first automated high throughput system for ...
(Date:9/2/2014)... 2014 /PRNewswire/ - BIOREM Inc. (TSXV: BRM) ("Biorem" or "the Company") ... consist of three large odour control systems for waste water ... "These orders are consistent with the market resurgence in ... President and CEO. "After a difficult period of restraint in ... to see sales growth. Our lower cost structure, technology improvements ...
Breaking Biology Technology:Linde helps fund STEM education in Massachusetts 2Linde helps fund STEM education in Massachusetts 3Press agenda for Project LIBERTY Grand Opening 2Press agenda for Project LIBERTY Grand Opening 3Press agenda for Project LIBERTY Grand Opening 4Press agenda for Project LIBERTY Grand Opening 5Opertech Bio Receives U.S. Patent for High-Throughput Taste Evaluation Technology 2
... , , ... , , , ... 2100 bioanalyzer and,the Cell Fluorescence LabChip kit can be used to ... on,Jurkat cells as well as CD4 staining on CCRF-CEM cells and ...
... , , ... , A fast and accurate method ... , , , This Application Note,addresses the ... quantity,and lifespan. Using the Agilent,2100 bioanalyzer and Cell Fluorescence,LabChip kit the ...
... , , ... , , , , ... can,be used to analyze apoptotic cell samples. Performance of two assays,for ... with a conventional flow cytometer. Histogram quality,of the low number of ...
Cached Biology Technology:Detection of antibody-stained cell,surface and intracellular protein targets,with the Agilent 2100 bioanalyzer 2Detection of antibody-stained cell,surface and intracellular protein targets,with the Agilent 2100 bioanalyzer 3Detection of antibody-stained cell,surface and intracellular protein targets,with the Agilent 2100 bioanalyzer 4Detection of antibody-stained cell,surface and intracellular protein targets,with the Agilent 2100 bioanalyzer 5Detection of antibody-stained cell,surface and intracellular protein targets,with the Agilent 2100 bioanalyzer 6Detection of antibody-stained cell,surface and intracellular protein targets,with the Agilent 2100 bioanalyzer 7Detection of antibody-stained cell,surface and intracellular protein targets,with the Agilent 2100 bioanalyzer 8Flow cytometric analysis of human,primary cells using the Agilent 2100,bioanalyzer and on-chip staining 2Flow cytometric analysis of human,primary cells using the Agilent 2100,bioanalyzer and on-chip staining 3Apoptosis detection by annexin V and,active caspase-3 with the Agilent 2100,bioanalyzer 2Apoptosis detection by annexin V and,active caspase-3 with the Agilent 2100,bioanalyzer 3Apoptosis detection by annexin V and,active caspase-3 with the Agilent 2100,bioanalyzer 4Apoptosis detection by annexin V and,active caspase-3 with the Agilent 2100,bioanalyzer 5Apoptosis detection by annexin V and,active caspase-3 with the Agilent 2100,bioanalyzer 6Apoptosis detection by annexin V and,active caspase-3 with the Agilent 2100,bioanalyzer 7Apoptosis detection by annexin V and,active caspase-3 with the Agilent 2100,bioanalyzer 8Apoptosis detection by annexin V and,active caspase-3 with the Agilent 2100,bioanalyzer 9
... The Partec Cell Counter Analyser counts cells ... the CCA detects only cells, so that ... the results.,While passing through a 250 m ... fluorescence light signal which is used as ...
... proteins are used to immunize rabbits. , Agro-Bio ... quantity of antigen and quality of the antibodies ... per protocol in order to avoid animal variability. ... is approximately 75 mL per rabbit.,150 to 250 ...
EPA II Buffer is a diluent formulated to stabilize the activity of Chemicon horseradish peroxidase (HRP)-antibody conjugates at high conjugate dilution for extended periods....
... an important role in the regulation of ... death. Splice variants have been reported that ... defined apoptotic pathways. With Apoptosis and Cell ... classical expression profiling and at the same ...
Biology Products: