Navigation Links
Enzyme trio for biosynthesis of hydrocarbon fuels
Date:6/21/2010

this year in the journal Applied and Environmental Microbiology, titled "Genes Involved in Long-Chain Alkene Biosynthesis in Micrococcus luteus."

It has long been known that certain types of bacteria are able to synthesize aliphatic hydrocarbons, which makes them promising sources of the enzymes needed to convert lignocellulose into advanced biofuels. However, until recently, little was known about the bacterial biosynthesis of non-isoprenoid hydrocarbons beyond a hypothesis that fatty acids are precursors. JBEI researchers in the Fuels Synthesis Division, which is headed by co-author Keasling, are using the tools of synthetic biology, and mathematical models of metabolism and gene regulation to engineer new microbes that can quickly and efficiently produce advanced biofuel molecules. E.coli is one of the model organisms being used in this effort because it is a well-studied microbe that is exceptionally amenable to genetic manipulation.

"We chose to work with M. luteus because a close bacterial relative was well-documented to synthesize alkenes and because a draft genome sequence of M. luteus was available," Beller says. "The first thing we did was to confirm that M. luteus also produces alkenes."

Beller and his colleagues worked from a hypothesis that known enzymes capable of catalyzing both decarboxylation and condensation should be good models for the kind of enzymes that might catalyze alkene synthesis from fatty acids. Using condensing enzymes as models, the scientists identified several candidate genes in M. luteus, including Mlut_13230. When expressed in E. coli together with the two adjacent genes - Mlut_13240 and 13250 - this trio of enzymes catalyzed the synthesis of alkenes from glucose. Observations were made both in vivo and in vitro.

"This group of enzymes can be used to make aliphatic hydrocarbons in an appropriate microbial host but the resulting alkenes are too long to be used d
'/>"/>

Contact: Lynn Yarris
lcyarris@lbl.gov
510-486-5375
DOE/Lawrence Berkeley National Laboratory
Source:Eurekalert  

Page: 1 2 3

Related biology news :

1. New technique reliably detects enzyme implicated in cancer and atherosclerosis
2. Protein regulates enzyme linked to Alzheimers disease
3. NIH funds multicenter glue grant to study enzyme function
4. Synthetic enzymes could help ID proteins
5. Pitt-led international study identifies human enzyme that breaks down potentially toxic nanomaterials, opens door to novel drug delivery
6. Pitt-led study identifies human enzyme that breaks down potentially toxic nanomaterials
7. Ayman El-Hattab, MD is awarded the 2010 Genzyme/ACMG Foundation Genetics Fellowship
8. Dangerous plaques in blood vessels rupture by overproducing protein-busting enzymes
9. Key enzyme discovered to be master regulator in protein-protein reactions
10. MSU scientists unlock key enzyme using newly created cool method
11. Enzyme deficiency protects hepatitis C patients from treatment-related anemia
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Enzyme trio for biosynthesis of hydrocarbon fuels
(Date:7/9/2014)... neuronal function and increase the survival rate of ... team, School of Pharmaceutical Sciences, Southwest University, China ... were administered with low dose of levothyroxine for ... rats exhibited an obvious improvement in cognitive and ... 93%. The underlying mechanism was demonstrated that levothyroxine ...
(Date:7/9/2014)... less fit in terms of cardiorespiratory capacity, strength ... southern adolescents are more obese and present higher ... from the centre-north of Europe. , These are ... study conducted by scientists from the University of ... 25 other European research groups. The study compared ...
(Date:7/9/2014)... Jena (Germany) They are amongst the most numerous inhabitants ... Emiliania huxleyi . Not visible to the naked eye, ... square kilometer sized patches, they are even visible on ... is responsible for approximately half of the global ... Friedrich Schiller University Jena (Germany). In the process the ...
Breaking Biology News(10 mins):Adolescents from southern Europe are less fit and more obese than central-northern European peers 2Short circuit in the food web 2
... gas could be produced from rotting organic matter like food ... pushing governments to use that ancient knowledge to turn household ... energy. Our environment would be the winner since less waste ... However, while the scientific theory is simple, treating the flow ...
... tricks and survival strategies of viruses to effectively ... virtual institute VISTRIE that received its funding commitment ... Immune Evasion", is a joint program grant with ... Association of German Research Centres. Coordinated by the ...
... ,As our bodies first form, developing cells are a lot ... addresses pinned to their shirts. The notes identify one as ... as a neuron. And that,s what they each grow up to ... original marching orders caused by aging, disease and other stressors like ...
Cached Biology News:Time to make more out of waste 2Time to make more out of waste 3Virtual Institute investigates virus infections 2Mutations can spur dangerous identity crisis in cells 2
(Date:1/15/2014)... device," Nobel laureate Herbert Kroemer famously observed, referring ... the junctures where layers of different materials meet. ... between layers of metal oxides are becoming increasingly ... spintronics, high-temperature superconductors, ferroelectrics and multiferroics. Realizing the ...
(Date:1/15/2014)... Jan. 15, 2014   Niagara Thermal Products is ... Buffalo BioBlower Technologies LLC ("B3") as the critical thermal ... award from the Army Corps of Engineers.  B3 has ... all biological contaminants and destroys VOCs and other chemical ...
(Date:1/15/2014)... SAN JOSE, California , January 15, 2014 ... for cancer, today announced the appointment of Thomas C Reynolds ... over 20 years, development experience gained in the biotechnology industry, ... "I am delighted to welcome Tom at this transformative ...
(Date:1/15/2014)... A study has been launched to test whether ... could help to tackle the problem of obesity.  This unique ... surgery based in Stowmarket) and academics at University Campus Suffolk ... technology, which is inspired by equipment used to collect data ...
Breaking Biology Technology:A deeper look at interfaces 2Niagara Thermal Products LLC Selected as Critical Supplier for Buffalo BioBlower Air Purification Systems 2Oxford BioTherapeutics Appoints Thomas C Reynolds MD, PhD to its Board of Directors 2Oxford BioTherapeutics Appoints Thomas C Reynolds MD, PhD to its Board of Directors 3Formula 1 Technology Tackles Obesity in Unique Healthcare Partnership 2Formula 1 Technology Tackles Obesity in Unique Healthcare Partnership 3Formula 1 Technology Tackles Obesity in Unique Healthcare Partnership 4
... CITY, QC, Sept. 10 /PRNewswire-FirstCall/ - Mr. Pierre,Fitzgibbon, ... ("Atrium" or the "Company", TSX: ATB) today announced ... follow the decision by Mr.,Richard Bordeleau, President of ... in order to pursue news challenges. However, Mr. ...
... MichBio, the association for,Michigan,s life sciences industry, announced ... in Lansing. Guided tours will be available to ... 1 of the Expo, October 16. Attendees must register ... credentials and maps to the,locations. Visitors will transport themselves ...
... the Journal Nature Medicine, CLEVELAND, Sept. 10 ... normal cholesterol levels, yet seemingly,misses striking others who smoke, ... help explain why one person suffers a heart attack,and ... Research Institute have,discovered a mechanism that contributes to the ...
Cached Biology Technology:Atrium Innovations Announces Management Changes 2MichBio Announces Expo Tours; Extends Deadline for Innovator Showcase Applications 2Cleveland Clinic-Led Research Uncovers New Mechanism That Heightens Risk of Heart Disease 2Cleveland Clinic-Led Research Uncovers New Mechanism That Heightens Risk of Heart Disease 3
Human HVEM/TNFRSF14 Allophycocyanin MAb (Clone 94801)...
Enodgenous alkaline phosphotase inhibitor. Levamisole solution is supplied in concentrated form (20x). The final concentration of levamisole in the substrate solution will be 1 mM....
X-gal,1g...
...
Biology Products: