Navigation Links
Engineered coral pigment helps scientists to observe protein movement
Date:7/27/2010

f the cell are then illuminated with laser light of specific wavelength, causing the marker proteins to emit light at another wavelength. This allows dynamical cell processes to be visualised and studied under the microscope.

In the native state, four molecules of IrisFP join together to form a tetramer, creating problems for fusion-protein applications. To get round this, the researchers have now modified the protein by introducing four mutations. This makes individual IrisFP molecules (monomers) more stable, reducing their tendency for form tetramers.

"The monomeric variant, mIrisFP, maintains dual photoactivation capacity and has excellent properties as a genetically encoded fluorescent marker protein," explained Wiedenmann.

To test the usefulness of mIrisFP, the researchers genetically fused it with a number of other proteins within cultured cells. These included transcription factors, which regulate the expression of genes within the cell nucleus, and constituent proteins of the cell skeleton ('cytoskeleton'). In all cases, the fusion proteins functioned normally.

Further experiments demonstrated that mIrisFP fusion proteins could, as hoped, be used to study dynamical processes within live cells with a spatial resolution beyond the limits of conventional light microscopy. Specifically, the researchers successfully combined so-called pulse-chase experiments with photoactivation localisation microscopy (PALM) imaging to follow the movement of fluorescently marked fusion proteins over time and at very high spatial resolution.

"The dual photoactivation capability and the monomeric nature of mIrisFP should allow cell biologists to perform a wider range of experiments than possible using only conventional PA-FPs," said Wiedenmann.

"Marine animals such as corals and anemones are not only beautiful and important for ecosystem functioning, but also as source of fluorescent proteins of enormous value to biomedical re
'/>"/>

Contact: Dr. Rory Howlett
r.howlett@noc.soton.ac.uk
44-023-805-98490
National Oceanography Centre, Southampton (UK)
Source:Eurekalert  

Page: 1 2 3

Related biology news :

1. Native-like spider silk produced in metabolically engineered bacterium
2. Genetically engineered crops benefit many farmers, but the technology needs proper management to remain effective
3. Genetically engineered tobacco plant cleans up environmental toxin
4. Engineered tobacco plants have more potential as a biofuel
5. Bioengineered materials promote the growth of functional vasculature, new study shows
6. First genetically-engineered malaria vaccine to enter human trials
7. Genetically engineered mice yield clues to knocking out cancer
8. Engineered pig stem cells bridge the mouse-human gap
9. Scientists test Moreton Bay as coral lifeboat
10. Scientific coral reef survey to be conducted in Bonaire
11. Study shows cleaner water mitigates climate change effects on Florida Keys coral reefs
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Engineered coral pigment helps scientists to observe protein movement
(Date:7/31/2014)... lurks beneath many of the roughly 156,000 gas ... is corrosion in parts of underground gas storage ... contamination of groundwater, a source of drinking water. ... have reported many rapidly corroding gas storage tank ... generally associated with use of gasoline-ethanol blends and ...
(Date:7/31/2014)... make a rational choice to be liberal or conservative? ... a matter of genetics?, Two political scientists from the ... say that neither conscious decision-making nor parental upbringing fully ... right. , A growing body of evidence shows that ... of political differences, the researchers say in the latest ...
(Date:7/31/2014)... known as the Rim Fire was in full fury, ... when it entered the backcountry of Yosemite National Park ... inside the park, the battle began to turn, enacting ... drought can combine to fuel large, severe fires. , ... encountered lands where fire had been used as a ...
Breaking Biology News(10 mins):NIST corrosion lab tests suggest need for underground gas tank retrofits 2Scientists find growing consensus: Political attitudes derive from body and mind 2The Rim Fire 1 year later: A natural experiment in fire ecology and management 2The Rim Fire 1 year later: A natural experiment in fire ecology and management 3The Rim Fire 1 year later: A natural experiment in fire ecology and management 4The Rim Fire 1 year later: A natural experiment in fire ecology and management 5
... are a major cause of disability or premature death in ... already suffered one fragility fracture; they are at twice the ... have not fractured. Nevertheless health care systems around ... leaving them exposed to debilitating and life-threatening secondary fractures. ...
... to 20 percent of veterans returning from Afghanistan and Iraq ... that nearly half these veterans may have a problem so ... it. A new study conducted by Charles W. Wilkinson, Elizabeth ... R. Peskind, all of the VA Puget Sound Health Care ...
... YORK , April 22, 2013 ... report is available in its catalogue: ... Opportunities, 2018 http://www.reportlinker.com/p01162444/India-Biometrics-Market-Forecast-and-Opportunities-2018.html#utm_source=prnewswire&utm_medium=pr&utm_campaign=Security_Systems ... new and progressive products in ...
Cached Biology News:New review sets international standards for best practice in fracture liaison services 2Nearly half of veterans found with blast concussions might have hormone deficiencies 2Nearly half of veterans found with blast concussions might have hormone deficiencies 3India Biometrics Market Forecast and Opportunities, 2018 2India Biometrics Market Forecast and Opportunities, 2018 3India Biometrics Market Forecast and Opportunities, 2018 4India Biometrics Market Forecast and Opportunities, 2018 5India Biometrics Market Forecast and Opportunities, 2018 6
(Date:7/31/2014)... (PRWEB) July 31, 2014 Food ... are increasingly searching for food alternatives that offer ... benefits. The food ingredient market has numerous players ... such as Vital Force Technology (VFT). VFT ... than 4,000 energetic patterns, all tested to produce ...
(Date:7/31/2014)... , July 31, 2014 Nearly 20,000 ... th Annual Meeting & Clinical Lab Expo in ... meeting featured never-before-seen breakthroughs in diagnostic research and technology ... they need. As of Wednesday, July 30, ... AACC Annual Meeting & Clinical Lab Expo, with more ...
(Date:7/31/2014)... through organs and even the entire body to visualize ... structures has been a long-time dream of biologists. A ... in the journal Cell has now made ... opaque organs, bodies, and human tissue biopsies transparent, while ... could pave the way for a better understanding of ...
(Date:7/31/2014)... July 31, 2014 The SNIS Foundation ... $25,000 to fund a translational research project.  The ... the gift was presented at the Society of ... in Colorado Springs, CO. ... approaches to neurovascular conditions, SNIS formed the SNIS ...
Breaking Biology Technology:Functional Ingredient Manufacturer – Vital Force Technology Infuses Raw Materials & Ingredients with Subtle Energy Formula 2Research on Digital Health, Alzheimer's, Ebola Draws Nearly 20,000 Attendees to 2014 AACC Annual Meeting & Clinical Lab Expo 2Research on Digital Health, Alzheimer's, Ebola Draws Nearly 20,000 Attendees to 2014 AACC Annual Meeting & Clinical Lab Expo 3See-through organs and bodies will accelerate biomedical discoveries 2SNIS Foundation Bestows First Grant Award For Cutting-Edge NeuroInterventional Research 2SNIS Foundation Bestows First Grant Award For Cutting-Edge NeuroInterventional Research 3
... , , SAN ... (NYSE: SAI ) announced today that Anthony Hess, Ph.D., ... Health Solutions BU (HSBU) effective Aug. 3. Hess will ... and commercial health customers, including the Departments of Defense, Health and ...
... PERTH, Australia, July 16 Dow AgroSciences LLC, a wholly owned ... and NemGenix, a Perth based biotechnology company, announced today a newly ... , , The collaboration will ... Financial terms of the agreement were not disclosed. , ...
... , COSTA MESA, Calif., July 16 Oxygen Biotherapeutics, ... on the first $5 million financing tranche with the Vatea Fund, Segregated ... , For additional details on the agreement, please ... which is available on the SEC website at www.sec.gov and through the ...
Cached Biology Technology:Anthony Hess to Lead SAIC Health Solutions Business Unit 2Anthony Hess to Lead SAIC Health Solutions Business Unit 3Dow AgroSciences, NemGenix Establish New Collaboration in Plant Biotechnology 2
Phospho-SEK1/MKK4 (Thr261) Antibody...
Rabbit polyclonal to Warfarin ( Abpromise for all tested applications). Antigen: Chemical / Small Molecule conjugated to KLH...
HSD17B3 Immunogen: HSD17B3 (NP_000188, 29 a.a. ~ 120 a.a) partial recombinant protein with GST tag. Storage: -20 C, Avoid Freeze/Thaw Cycles *Available for distribution in the US only...
Rabbit polyclonal to 13,14 dihydro 15 keto Prostaglandin E2 ( Abpromise for all tested applications)....
Biology Products: