Navigation Links
Energy-saving chaperon Hsp90
Date:1/13/2012

A special group of proteins, the so-called chaperons, helps other proteins to obtain their correct conformation. Until now scientists supposed that hydrolyzing ATP provides the energy for the large conformational changes of chaperon Hsp90. Now a research team from the Nanosystems Initiative Munich could prove that Hsp90 utilizes thermal fluctuations as the driving force for its conformational changes. The renowned journal PNAS reports on their findings.

ATP is the major energy source for most organisms and ATPases are the machines, which utilize this fuel, for example to move muscles or cargo in our body. The very abundant chaperone protein Hsp90 has such an ATPase in each of its two monomers. During the last years experiments had suggested that the movement and conformational changes of ATPase proteins are in general strictly linked to ATP binding and hydrolysis (i.e. fuel consumption).

To probe this theory Thorsten Hugel, Professor at the Technische Universitaet Muenchen (TUM) and member of the Nanosystems Initiative Munich (NIM), and his team designed a special three color single-molecule FRET (Frster resonance energy transfer) assay with alternating laser excitation (ALEX) for simultaneous observation of ATP binding and conformational changes. Unexpectedly the experiments revealed that binding and hydrolysis of ATP is not correlated with the large conformational changes of Hsp90. Hsp90 is instead a highly flexible machinery driven by thermal fluctuations.

"Thermal fluctuations are random changes in the structure of the protein they can be thought of as collisions with water molecules in the environment, which move rather violently at the temperatures in a living organism," says Thorsten Hugel. "Using these clashes to switch back and forth between different conformations, saves Hsp90 valuable ATP." But then what is the task of ATPase in the Hsp90 chaperone? The scientists suspect that co-chaperones and substrate proteins alter the system so that ATP binding or hydrolysis can take a crucial task.

With the newly developed experimental setup, it is now possible to investigate the very complex system in greater detail to resolve this important question. The Munich biophysicists therewith offer a new perspective on the energy conversion in molecular machines.


'/>"/>

Contact: Dr. Andreas Battenberg
battenberg@zv.tum.de
49-892-891-0510
Technische Universitaet Muenchen
Source:Eurekalert  

Related biology news :

1. Energy-saving powder
2. Energy-saving method checks refrigerant level in air conditioners
3. A chaperone for the guardian of the genome
4. Molecular chaperones traffic signaling proteins between cells in plant stem-cell maintenance pathway
5. A chaperone system guides tail-anchored membrane proteins to their destined membrane
6. Common chaperone protein found to work in surprising way, say Scripps Research scientists
7. Blame the chaperone
8. Dynamics of chaperone protein critical in rescuing brains of Alzheimers mice from neuron damage
9. Breakthrough: With a chaperone, copper breaks through
10. Molecular chaperone keeps bacterial proteins from slow-dancing to destruction
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Energy-saving chaperon Hsp90
(Date:4/13/2017)... According to a new market research report "Consumer IAM Market by Solution ... Authentication Type, Deployment Mode, Vertical, and Region - Global Forecast to 2022", ... 14.30 Billion in 2017 to USD 31.75 Billion by 2022, at a ... ... MarketsandMarkets Logo ...
(Date:4/11/2017)... 11, 2017 No two people are ... the New York University Tandon School of Engineering ... found that partial similarities between prints are common ... mobile phones and other electronic devices can be ... vulnerability lies in the fact that fingerprint-based authentication ...
(Date:4/5/2017)... , April 5, 2017  The Allen Institute for ... Cell Explorer: a one-of-a-kind portal and dynamic digital window ... imaging data, the first application of deep learning to ... stem cell lines and a growing suite of powerful ... for these and future publicly available resources created and ...
Breaking Biology News(10 mins):
(Date:5/18/2017)... Malden, Mass. (PRWEB) , ... May 18, 2017 ... ... completed the procedure on April 28, 2017 at the Prince Of Wales Private ... degenerative cervical disc at level C6-C7. The patient failed conservative treatments prior to ...
(Date:5/18/2017)... ... May 16, 2017 , ... Clinical Supplies Management (“CSM”), a Great ... company continues to grow. CSM has doubled in size over the past six ... aggressive growth strategy. , Roger Gasper joins CSM as Chief Financial Officer. Roger ...
(Date:5/18/2017)... ... May 17, 2017 , ... ... enhances its scientific power by providing investigators access to a high-profile scientific ... join the scientific advisory board. “We are committed to offering superior services ...
(Date:5/18/2017)... SANTA BARBARA, CALIFORNIA (PRWEB) , ... May 17, ... ... risk management, technological innovation and business process optimization firm for the life sciences ... the Chairman of the UDIs and Traceability for Medical Devices conference in Brussels, ...
Breaking Biology Technology: