Navigation Links
Early exposure to common weed killer impairs amphibian development
Date:4/16/2008

lopment, according to previous research.

To study the consequences of atrazine exposure during organ morphogenesis, McLaughlin and her colleagues, Professor of Biology J. Michael Reed, doctoral candidate Jenny R. Lenkowski and Lisa Deininger, a Summer Scholars program undergraduate student, collected eggs from adult female frogs and then fertilized them in vitro. Scientists exposed the developing tadpoles to 10, 25 and 35 mg/L of atrazine. The 35 mg/L dosage simulated the average amount of herbicide used when it is applied in the field, said McLaughlin.

Multiple Impacts

Twelve to 24 hours after exposure to atrazine, tadpoles were examined for abnormal heart growth, visceral hemorrhaging, intestinal coiling, edema and apoptosis (normal cell death that is "programmed" by the body).

Compared with control populations, the tadpoles that were exposed to atrazine had a dramatically higher incidence of abnormalities. The degree of deformities generally corresponded to the size of the dose. After 48 hours of exposure, the point at which organ development is disrupted most profoundly, 57 percent of the tadpoles exposed to 35 mg/L of atrazine had hearts that were smaller than normal, compared with 2% to 3% for the two control groups.

Ectopic Cell Death

The Tufts scientists also examined atrazine exposed tadpoles for increased incidence of apoptosis by measuring levels of active caspase-3 in the pronephric kidney and midbrain. Caspase-3 is a protein needed for apoptosis to occur. They conducted measurements after 6, 12, 24 and 48 hours of exposure in tadpoles exposed to 25 and 35 mg/l of atrazine. Researchers observed that the atrazine-exposed tadpoles showed significant increases in caspase-3 levels in the kidney and midbrain at 12 hours and beyond when compared with controls. The findings indicated a high incidence of ectopic, or abnormal, apoptosis.

"The increased levels of apoptosis in th
'/>"/>

Contact: Alex Reid
alexander.reid@tufts.edu
617-627-4173
Tufts University
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. 60 second test could help early diagnosis of common brain diseases
2. Restless legs syndrome affects nearly 2 percent of US/UK children
3. Bleeding, not inflammation, is major cause of early lung infection death
4. Pig study sheds new light on the colonisation of Europe by early farmers
5. Color contrast is seen by the brain early doors
6. Extra gene copies were enough to make early humans mouths water
7. Was ability to run early mans Achilles heel?
8. New technique can be breakthrough for early cancer diagnosis
9. Yam bean a nearly forgotten crop
10. NIH awards nearly $23M to University of Chicago for translational research
11. Yam bean a nearly forgotten crop
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:10/14/2014)... published in Cancer Research shows SIRT6—a protein ... cancers—can promote the development of skin cancers by turning ... of sun-damaged skin cells. , Previously considered protective, SIRT6 ... sirtuins that help regulate genomic stability and prevent some ... repair DNA damage, which can lead to cancer. This ...
(Date:10/14/2014)... the house fly genome for the first time, revealing ... insect that thrives in pathogen-rich dung piles and garbage ... journal Genome Biology , will increase understanding of ... quickly adapt to resist insecticides, which could lead to ... carry and transmit more than 100 human and animal ...
(Date:10/14/2014)... Mount Sinai-led research team has discovered a new kind of ... a cell that lines liver blood vessels, according to a ... . The existence of such a cell type contradicts current ... embryo, and may hold clues to origins of, and future ... from a single cell into a complex being made up ...
Breaking Biology News(10 mins):Two-faced gene: SIRT6 prevents some cancers but promotes sun-induced skin cancer 2House fly genome reveals expanded immune system 2Stem cell discovery challenges dogma on how fetus develops; holds insights for liver cancer and reg 2Stem cell discovery challenges dogma on how fetus develops; holds insights for liver cancer and reg 3
... 19, 2012 A new study by scientists on ... in cell culture, a natural compound can virtually eliminate ... defines a novel class of HIV anti-viral drugs endowed ... and chronically infected cells. The HIV/AIDS pandemic continues ...
... , New Rochelle, NY, July 19, 2012To enable greater ... approaches such as co-firing of high percentages of biomass ... A comprehensive review of the strategies currently available and ... presented in I ndustrial Biotechnology , a peer-reviewed journal ...
... The Wyss Institute for Biologically Inspired Engineering at Harvard ... million contract (including option) from the Defense Advanced Research ... helps improve physical endurance for soldiers in the field. ... onset of fatigue, enabling soldiers to walk longer distances, ...
Cached Biology News:Scripps Research scientists show potent new compound virtually eliminates HIV in cell culture 2Strategies to improve renewable energy feedstocks 2Harvard's Wyss Institute to develop smart suit that improves soldiers' physical endurance 2
(Date:10/25/2014)... Durham, NC (PRWEB) October 24, 2014 ... appears to overcome several pitfalls of current skin grafting ... scarring and produces hair. The method, outlined in the ... a breakthrough for treating deep skin injuries that result ... skin injury completely destroys the skin’s regenerative elements. These ...
(Date:10/25/2014)... , October 24, 2014 Oramed ... pharmaceutical company focused on the development of oral drug ... Nadav Kidron will present at FireRock Capital,s Micro-Cap ... Annual Micro-Cap Conference ,Date: Tuesday, October 28, 2014 ,Time: ... New York City , ...
(Date:10/25/2014)... Biopharmaceuticals, Inc. (OTCBB: RGRX) ("the Company" or "RegeneRx") today ... on Thymosins in Health and Disease in ... beta 4 (TB4) linked with rhodamine (a fluorescent tracer ... rodent model of stroke, it was visualized outside of ... tissue of the brain).   The leakage of TB4 in ...
(Date:10/25/2014)... 2014 Research and Markets has ... Product , Research Area & by End User - ... In this report, the global epigenetics ... areas, and end users. The product segments of the ... & consumables, kits, and reagents. The subsegments of the ...
Breaking Biology Technology:New engineering method shows promise for faster healing, more cosmetically appealing skin grafts 2New engineering method shows promise for faster healing, more cosmetically appealing skin grafts 3Oramed to Present at the FireRock Annual Micro-Cap Conference 2Oramed to Present at the FireRock Annual Micro-Cap Conference 3Researchers Report Thymosin Beta 4 Crosses Blood-Brain Barrier In Animal Stroke Model 2Global Epigenetics (VLA, WBA, NBA, RTA, Rotary-Wing Aircraft) Market - Forecast to 2019 2Global Epigenetics (VLA, WBA, NBA, RTA, Rotary-Wing Aircraft) Market - Forecast to 2019 3
... difficult transfections when using the,complete control kit ... , We describe the vector pFB-ERV, an MMLV-based ... proteins RXR and VgEcR.,In stable cell lines infected ... induction ratios of greater than 200-fold are attainable ...
... One column purification of protein with native amino ... Wyborski * John C. Bauer * Barbara McGowan ... Systems, Inc. , Stratagene has improved the Affinity ... the pCAL-n-EK vector. This E. coli,cloning and expression ...
... highly specific digestion of purified,proteins or proteins in ... Claycomb * ChaoFeng Zheng * Denise Wyborski,* John ... Bernardino * Peter Vaillancourt ,Stratagene Cloning Systems, Inc. ... that cleaves at the carboxy terminus of its ...
Cached Biology Technology:pFB-ERV: Retroviral Delivery of the Ecdysone Receptor Proteins 2pFB-ERV: Retroviral Delivery of the Ecdysone Receptor Proteins 3pFB-ERV: Retroviral Delivery of the Ecdysone Receptor Proteins 4pFB-ERV: Retroviral Delivery of the Ecdysone Receptor Proteins 5pFB-ERV: Retroviral Delivery of the Ecdysone Receptor Proteins 6Expression and Purification of Recombinant Proteins That Have Native Amino,Acid Sequence 2Expression and Purification of Recombinant Proteins That Have Native Amino,Acid Sequence 3Expression and Purification of Recombinant Proteins That Have Native Amino,Acid Sequence 4Expression and Purification of Recombinant Proteins That Have Native Amino,Acid Sequence 5Expression and Purification of Recombinant Proteins That Have Native Amino,Acid Sequence 6Expression and Purification of Recombinant Proteins That Have Native Amino,Acid Sequence 7Expression and Purification of Recombinant Proteins That Have Native Amino,Acid Sequence 8Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini 2Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini 3Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini 4
... has a long and outstanding history ... Several years ago we became aware ... was being modified to inject Xenopus ... redesigning the microdispenser to eliminate any ...
... power supply (Cat. No. Minis 150VS) is ... meet most electrophoresis needs in a personal, ... 150V power supply is capable of running ... designed for individuals to have his/her own ...
BD BioCoat Laminin 35 mm Culture Dishes, tissue-culture treated polystyrene with a uniform application of mouse laminin....
... a powerful application for DNA and ... interface, Expression makes complex computational analyses ... uses the very latest computing technology ... way sequences are analysed. Features include: ...
Biology Products: