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Dynamic culture of a thermosensitive collagen hydrogel improves tissue-engineered peripheral nerve

Tissue engineering technologies offer new treatment strategies for the repair of peripheral nerve injury, but cell loss between seeding and adhesion to the scaffold remains inevitable. In a study reported on the Neural Regeneration Research (Vol. 9, No. 14, 2014), a thermosensitive collagen hydrogel remained as a liquid when kept at temperatures below 10C and gelled when the temperature was increased to 37C in an incubator for 30 minutes, which was used as an extracellular matrix and combined with bone marrow mesenchymal stem cells to construct tissue-engineered peripheral nerve composites in vitro. Results from DNA analysis and scanning electron microscope indicate that tissue-engineered composites formed with thermosensitive collagen hydrogel in dynamic culture can maintain larger numbers of seeded cells by avoiding cell loss during the initial adhesion stage; moreover, seeded cells are well-distributed throughout the material, which is of great significance for in vitro construction of tissue-engineered peripheral nerve composites.


Contact: Meng Zhao
Neural Regeneration Research

Page: 1

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