Navigation Links
Duke software dramatically speeds enzyme design
Date:2/16/2009

more like they would in real life. Moreover, it simultaneously evaluates what he called "an entire album, or ensemble," of possible shape shifting.

"Thus it not only addresses flexibility. It embraces it," Donald said." "So we might be able to quickly discover things that would take a very long time through purely experimental techniques. It should, in principle, be possible to redesign any enzyme simply by inputting the protein's shape into the algorithm and telling it what you want it to do."

The new report is the latest milestone in a nearly 10-year effort to develop reliable enzyme design algorithms, Donald said. In the process, his group has had a long collaboration with Amy Anderson of the University of Connecticut to biochemically test the algorithm's accuracy using the Gramicidin S Synthetase enzyme system, which produces the natural antibiotic in Bacillus brevis bacteria.

In the new PNAS report, Ivelin Georgiev, Donald's computer science graduate student who is one of K*'s designers, used the latest version of the algorithm to redesign the first step in the biochemical assembly line needed to make the antibiotic.

"Redesigning that first step is a big achievement," Donald said. "We are now beginning work on redesigning the half dozen subsequent steps downstream."

The algorithm includes a "dead-end elimination" feature that can run though all possible chemical interactions and flexible molecular architectures to weed out scenarios that cannot work. Calculating just one redesign might take up to a week in the 230-processor computer cluster housed in Donald's lab, he said.

After all the calculations were completed, biochemist Cheng-Yu Chen, another of Donald's graduate students, confirmed the algorithm's predicted designs in Donald's biochemical wet lab, using bacteria to synthesize some "quite big and tricky proteins," Donald said. "It was not at all trivial to do that, and testing the functions of
'/>"/>

Contact: Monte Basgall
monte.basgall@duke.edu
919-681-8057
Duke University
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Radiant Systems Selects BIO-key(R) Biometric Software for POS Solution
2. IdentiPHI Re Launches SAFmodule Software to Secure Novell(R) Networks
3. IdentiPHI Re Launches SAFmodule Software to Secure Novell(R) Networks
4. Radiant Systems Selects BIO-key(R) Biometric Software for POS Solution
5. Aware, Inc. Introduces Biometrics Software for Tenprint Autocapture
6. CIC Again Named to Software Magazines Annual Prestigious Software 500 List
7. Titanium Group Signs Letter of Intent to Acquire Multimilion Dollar Medical Software Company and Its Existing Sales Network
8. Titanium Group Signs Letter of Intent to Acquire Multimilion Dollar Medical Software Company and Its Existing Sales Network
9. Software Techniques Inc. Selects M2SYS Fingerprint Software to Meet Continuous Growth and Demand for Desktop Biometric Time & Attendance Solutions
10. LOGICARE Selects M2SYS Biometric Fingerprint Software to Ensure Patient Privacy and Protect Confidential Information
11. M2SYS Introduces Revolutionary Biometric Artificial Intelligence Technology to Significantly Reduce Fingerprint Software False Reject Rates
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:7/23/2014)... news release is available in German . ... and stability to cells, drive metabolic processes and transmit ... complex three-dimensional shapes. Scientists at the Max Planck Institute ... proteins can be constructed of similar amino acid chains ... that the proteins that exist today arose from common ...
(Date:7/23/2014)... BioCatch, the global leader in ... has been named in Gartner,s Market Guide for Online ... well-known Magic Quadrant for Web Fraud Detection. ... several vendors to deliver passive biometric analysis and predicted, ... feature of at least 30% of one-stop fraud detection ...
(Date:7/23/2014)... clinical use of induced pluripotent stem cell (iPSC) ... hindered by the risk of dysregulated cell growth, ... use etoposide treatment to halt teratoma formation in ... acute myocardial infarction, is demonstrated in an article ... peer-reviewed journal from Mary Ann Liebert, Inc., publishers. ...
Breaking Biology News(10 mins):Protein evolution follows a modular principle 2BioCatch Featured in Gartner's 2014 Online Fraud Detection Market Guide 2BioCatch Featured in Gartner's 2014 Online Fraud Detection Market Guide 3
... January 2014 Scientists from the Montreal Neurological ... two genes linked to hereditary Parkinson,s disease are ... The protective mechanism, which is reported in T ... arise from oxidative stress from mitochondria., "PINK1 and ...
... 21, JoVE, the Journal of Visualized Experiments , ... explored one of the most intriguing research questions facing ... protocol, titled Conducting Miller-Urey Experiments, is comprised of a ... Dr. Stanley Miller and Dr. Harold Urey in 1953. ...
... viral pathogen that typically infects plants has been found ... Researchers working in the U.S. and Beijing, China report ... journal of the American Society for Microbiology. The ... "resulted in the serendipitous detection of Tobacco Ringspot Virus, ...
Cached Biology News:Quality control of mitochondria as a defense against disease 2A 21st century adaptation of the Miller-Urey origin of life experiments 2Pathogenic plant virus jumps to honeybees 2
(Date:7/23/2014)... 23, 2014 StemGenex® , the ... the US aimed at improving the lives of patients ... study for Parkinson’s disease. StemGenex believes that a ... therapy are paramount when providing care to patients with ... stem cell therapy accessible to the millions of individuals ...
(Date:7/23/2014)... 2014 /PRNewswire/ - BioAmber Inc. (NYSE: ... chemicals, today announced that the underwriters of its common ... purchase an additional 420,000 shares of common stock ... share, less underwriting discounts and commissions, in connection ... 2,800,000 shares of common stock, bringing the expected ...
(Date:7/23/2014)... , July 23, 2014  GrowBLOX Sciences, Inc. (OTCQB: ... that it is filing for two medical marijuana dispensary locations ... are in addition to the Clark County, Nevada ... special use permit, as previously announced.  GrowBLOX Sciences, ... be submitting two new applications for dispensaries in the ...
(Date:7/23/2014)... With the ability to record continuously at over ... 15 minutes, Fastec Imaging has brought the same ease-of-use of ... “Finally, a high speed camera that can be used as ... lives.” proclaims Matt Kearney, VP of Sales for Fastec Imaging. ... and simplify the traditionally complicated workflow of a high speed ...
Breaking Biology Technology:StemGenex® Gives Hope to Parkinson’s Patients through New Stem Cell Clinical Study 2StemGenex® Gives Hope to Parkinson’s Patients through New Stem Cell Clinical Study 3BioAmber announces exercise in full of underwriters' option in connection with previously priced follow-on offering 2BioAmber announces exercise in full of underwriters' option in connection with previously priced follow-on offering 3GrowBLOX Sciences, Inc. Files for Two Additional Medical Marijuana Dispensaries in Las Vegas 2GrowBLOX Sciences, Inc. Files for Two Additional Medical Marijuana Dispensaries in Las Vegas 3Fastec Imaging Introduces the First High Speed Camcorder 2Fastec Imaging Introduces the First High Speed Camcorder 3
... /PRNewswire-FirstCall/ - On August 20, 2008,Oncothyreon Inc. (Nasdaq: ... a,letter from The Nasdaq Stock Market indicating that (i) ... on The NASDAQ Global Market,because it did not meet ... other things, that the market value of,its common stock ...
... with international ... and technology companies, TORONTO, Sept. 17 Aurillion, a ... Moreno as its vice president of sales and marketing. In ... grow the company,s business throughout the Americas, Europe,and Asia. He ...
... 17 Sigma-Aldrich Corporation,(Nasdaq: SIAL ) will be ... Wednesday, September 24th at 8:30 AM ET at the ... via live audio broadcast over the,Internet available at ... this file. For the webcast on, http://ir.sigmaaldrich.com users ...
Cached Biology Technology:Oncothyreon announces receipt of NASDAQ letter regarding compliance with NASDAQ Global Market listing standards 2Oncothyreon announces receipt of NASDAQ letter regarding compliance with NASDAQ Global Market listing standards 3Guillermo Moreno Named Aurillion's Vice President of Sales and Marketing 2
... Roches RTS AviTag E. coli Biotinylation Kit, Linear ... 100 E. coli expression kits. (Cat. No. 3 ... reagents to rapidly generate linear expression constructs by ... based on a combination of T7 RNA polymerase ...
... (GR) Competitor Assay Kits are ideal for ... The PolarScreen GR Competitor Assay Kits contain ... and one of the following proprietary fluorescent ... GS Red (red assay kit), or GS ...
... E. coli Biotinylation Kit, Plasmid is designed for ... coli expression kits. (Cat. No. 3 186 148, ... provides vectors for the easy introduction of a ... protein can be enzymatically mono-biotinylated by E. coli ...
... Exiqons LNA technology enables sensitive, fast ... on intact chromosomes. Short hybridisation ... fixed cells and chromosome spreads Multiplex ... Pre-validated probes Probes available ...
Biology Products: