Navigation Links
Duke researchers engineer cartilage from pluripotent stem cells
Date:10/29/2012

DURHAM, N.C. A team of Duke Medicine researchers has engineered cartilage from induced pluripotent stem cells that were successfully grown and sorted for use in tissue repair and studies into cartilage injury and osteoarthritis.

The finding is reported online Oct. 29, 2012, in the journal the Proceedings of the National Academy of Sciences, and suggests that induced pluripotent stem cells, or iPSCs, may be a viable source of patient-specific articular cartilage tissue.

"This technique of creating induced pluripotent stem cells an achievement honored with this year's Nobel Prize in medicine for Shimya Yamanaka of Kyoto University - is a way to take adult stem cells and convert them so they have the properties of embryonic stem cells," said Farshid Guilak, PhD, Laszlo Ormandy Professor of Orthopaedic Surgery at Duke and senior author of the study.

"Adult stems cells are limited in what they can do, and embryonic stem cells have ethical issues," Guilak said. "What this research shows in a mouse model is the ability to create an unlimited supply of stem cells that can turn into any type of tissue in this case cartilage, which has no ability to regenerate by itself."

Articular cartilage is the shock absorber tissue in joints that makes it possible to walk, climb stairs, jump and perform daily activities without pain. But ordinary wear-and-tear or an injury can diminish its effectiveness and progress to osteoarthritis. Because articular cartilage has a poor capacity for repair, damage and osteoarthritis are leading causes of impairment in older people and often requires joint replacement.

In their study, the Duke researchers, led by Brian O. Diekman, PhD., a post-doctoral associate in orthopaedic surgery, aimed to apply recent technologies that have made iPSCs a promising alternative to other tissue engineering techniques, which use adult stem cells derived from the bone marrow or fat tissue.

One challenge the researchers sought to overcome was developing a uniformly differentiated population of chondrocytes, cells that produce collagen and maintain cartilage, while culling other types of cells that the powerful iPSCs could form.

To achieve that, the researchers induced chondrocyte differentiation in iPSCs derived from adult mouse fibroblasts by treating cultures with a growth medium. They also tailored the cells to express green fluorescent protein only when the cells successfully became chondrocytes. As the iPSCs differentiated, the chondrocyte cells that glowed with the green fluorescent protein were easily identified and sorted from the undesired cells.

The tailored cells also produced greater amounts of cartilage components, including collagen, and showed the characteristic stiffness of native cartilage, suggesting they would work well repairing cartilage defects in the body.

"This was a multi-step approach, with the initial differentiation, then sorting, and then proceeding to make the tissue," Diekman said. "What this shows is that iPSCs can be used to make high quality cartilage, either for replacement tissue or as a way to study disease and potential treatments."

Diekman and Guilak said the next phase of the research will be to use human iPSCs to test the cartilage-growing technique.

"The advantage of this technique is that we can grow a continuous supply of cartilage in a dish," Guilak said. "In addition to cell-based therapies, iPSC technology can also provide patient-specific cell and tissue models that could be used to screen for drugs to treat osteoarthritis, which right now does not have a cure or an effective therapy to inhibit cartilage loss."


'/>"/>
Contact: Sarah Avery
sarah.avery@duke.edu
919-660-1306
Duke University Medical Center
Source:Eurekalert

Related biology news :

1. NIH researchers identify novel genes that may drive rare, aggressive form of uterine cancer
2. Researchers decipher the mecanism of membrane fission
3. SDSU researchers to study Chinas national treasure
4. Exercise boosts satisfaction with life, researchers find
5. UC Davis researchers develop new drug delivery system for bladder cancer using nanoparticles
6. Far from random, evolution follows a predictable genetic pattern, Princeton researchers find
7. USF researchers identify gene mutation linked to old age hearing loss
8. USDA scientists collaborate with global researchers to advance the mapping of the barley genome
9. Danish researchers release ground-breaking knowledge about calcium pumps in cells
10. CU-Boulder researchers uncover new target for cancer research
11. Columbia researchers report novel approach for single molecule electronic DNA sequencing
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:1/19/2017)... , Jan. 19, 2017 Sensory ... experience and security for consumer electronics, and ... processing systems and cybersecurity solutions, today announced a ... and financial institutions worldwide to bolster security of ... end-to-end secure user authentication platforms they offer, innerCore ...
(Date:1/12/2017)... -- New research undertaken by Fit Small Business has ... were simply asked which office technology had they not used ... issue.  Insights on what will be key features ... and industry leaders including Penelope Trunk , Martin ... Some of these findings included; ...
(Date:1/6/2017)... 2017  SomaLogic announced today that it has ... by iCarbonX, the China -based ... Digital Health Ecosystem that can define each person,s ... biological, behavioral and psychological data, the Internet and ... SomaLogic will provide proteomics data and applications expertise ...
Breaking Biology News(10 mins):
(Date:1/24/2017)... ... January 23, 2017 , ... The Foundation for Food ... seeks outstanding early career nominees for the 2017 New Innovator in Food and ... of a world in which one in nine people suffers from hunger. The ...
(Date:1/24/2017)... 2017 According to a new ... (Genetic, Cell-based (CD34, PBMC, BLT)), Application (Neuroscience, Hematopoiesis, Oncology, ... Biotech Companies, CRO) - Global Forecast to 2021", published ... Model Market for the forecast period of 2016 to ... Million by 2021 from USD 73.3 Million in 2016, ...
(Date:1/23/2017)... ... January 23, 2017 , ... USARAD ... announces significant growth last year adding 65 new US and international facilities to ... medical centers as well as one of US largest imaging center chains. USARAD ...
(Date:1/23/2017)... , January 23, 2017 Atlas Genetics ... completion of its Series D financing, raising $35 million from ... investor, Wondfo Biotech. ... Development of the Atlas Genetics io┬« system has ... trachomatis (CT) test announced in February 2016.  This new Series ...
Breaking Biology Technology: