Navigation Links
Drug-proof zebrafish reveal secrets of addiction
Date:7/31/2009

The effects of amphetamines on gene expression in zebrafish have been uncovered. This new study, published in BioMed Central's open access journal Genome Biology, provides clues to the genetics that underlie susceptibility to addiction by describing the nad zebrafish mutant, which does not feel the rewarding effects of the drugs.

Katharine Webb, from the German Research Center for Environmental Health, Helmholtz Zentrum Mnchen, worked with an international team of researchers to carry out the experiments. She said, "Addictive drugs all trigger a sequence of widespread long-lasting consequences on brain physiology, most of which are only partially understood. Because a major step in the development of addiction is the switch from drug use to drug abuse, we aimed to gain insight into the mechanisms triggering the initiation of addictive behaviour".

The team used the mutagenic chemical ENU to generate hundreds of mutant zebrafish. From these, they bred a line that did not respond to amphetamine administration (despite the presence of the drug in the fish's brain) but that appeared to be normal in all other ways. As amphetamine is experienced as pleasurable, amphetamine response was determined by measuring whether fish chose to move to a half of the tank where the drug had been given out.

By comparing these drug-proof nad mutants to fish with a normal response, Webb and her colleagues discovered a set of 139 genes that respond inappropriately to amphetamine in nad mutants, without being altered under normal conditions in either genotype. In addition to genes involved in pathways classically associated with reward, this gene set shows a striking enrichment in transcription factors that are specifically known for their involvement in brain development. Even more interestingly, as the authors demonstrate, several of these genes are expressed in neurogenic domains of the adult fish brain these are domains where neurons are generated from neural stem cells during adulthood. According to the researchers, " These factors, which are also dramatically down-regulated by amphetamine, can serve as valuable new entry points into studying the link between adult neurogenesis and addiction".

These results identify a new network of coordinated gene regulation that influences the response to amphetamine and may underlie the susceptibility to addiction.


'/>"/>

Contact: Graeme Baldwin
graeme.baldwin@biomedcentral.com
44-020-319-22165
BioMed Central
Source:Eurekalert

Related biology news :

1. Pitt team finds molecule that regulates heart size by using zebrafish screening model
2. Zebrafish provide a model for cancerous melanoma in humans
3. Alzheimer cell death in Zebrafish: Demise of neurons observed live for the first time
4. AZTI-Tecnalia coordinates a platform promoting the use of zebrafish in scientific research
5. NIH grants Phylonix Phase II SBIR to develop high-throughput in vivo zebrafish assays
6. Digital zebrafish embryo provides the first complete developmental blueprint of a vertebrate
7. Deadly genetic disease prevented before birth in zebrafish
8. A screening strategy using zebrafish targets genes that protect against hearing loss
9. Study shows how the zebrafish gets his stripe
10. Zebrafish to shed light on human mitochondrial diseases
11. Structure of protective protein in the eye lens revealed
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/2/2016)... The Department of Transport Management (DOTM) of ... Dollar project, for the , Supply and Delivery ... IT Infrastructure , to Decatur ... Identity Management Solutions. Numerous renowned international vendors participated in the ... was selected for the most compliant and innovative solution. The ...
(Date:5/24/2016)... patient care by providing unparalleled technology to leaders of the medical imaging industry.  As ... added to the range of products distributed by Ampronix. Photo - ... ... ... ...
(Date:5/9/2016)... DUBAI , UAE, May 9, 2016 ... choice when it comes to expanding freedom for high ... Even in today,s globally connected world, there ... online conferencing system could ever duplicate sealing your deal ... are obtaining second passports by taking advantage of citizenship ...
Breaking Biology News(10 mins):
(Date:6/23/2016)... ... 2016 , ... Mosio, a leader in clinical research patient ... and Retention Tips.” Partnering with experienced clinical research professionals, Mosio revisits the hurdle ... and strategies for clinical researchers. , “The landscape of how patients receive and ...
(Date:6/23/2016)... 2016   Boston Biomedical , an industry ... to target cancer stemness pathways, announced that its ... Drug Designation from the U.S. Food and Drug ... including gastroesophageal junction (GEJ) cancer. Napabucasin is an ... cancer stemness pathways by targeting STAT3, and is ...
(Date:6/23/2016)... June 23, 2016 A person commits a crime, ... scene to track the criminal down. An outbreak ... and Drug Administration (FDA) uses DNA evidence to track down ... Sound far-fetched? It,s not. The FDA has increasingly used ... investigations of foodborne illnesses. Put as simply as possible, whole ...
(Date:6/23/2016)... ... 2016 , ... Charm Sciences, Inc. is pleased to announce ... Research Institute approval 061601. , “This is another AOAC-RI approval of the Peel ... President of Regulatory and Industrial Affairs. “The Peel Plate methods perform comparably to ...
Breaking Biology Technology: