Navigation Links
Drug-proof zebrafish reveal secrets of addiction
Date:7/31/2009

The effects of amphetamines on gene expression in zebrafish have been uncovered. This new study, published in BioMed Central's open access journal Genome Biology, provides clues to the genetics that underlie susceptibility to addiction by describing the nad zebrafish mutant, which does not feel the rewarding effects of the drugs.

Katharine Webb, from the German Research Center for Environmental Health, Helmholtz Zentrum Mnchen, worked with an international team of researchers to carry out the experiments. She said, "Addictive drugs all trigger a sequence of widespread long-lasting consequences on brain physiology, most of which are only partially understood. Because a major step in the development of addiction is the switch from drug use to drug abuse, we aimed to gain insight into the mechanisms triggering the initiation of addictive behaviour".

The team used the mutagenic chemical ENU to generate hundreds of mutant zebrafish. From these, they bred a line that did not respond to amphetamine administration (despite the presence of the drug in the fish's brain) but that appeared to be normal in all other ways. As amphetamine is experienced as pleasurable, amphetamine response was determined by measuring whether fish chose to move to a half of the tank where the drug had been given out.

By comparing these drug-proof nad mutants to fish with a normal response, Webb and her colleagues discovered a set of 139 genes that respond inappropriately to amphetamine in nad mutants, without being altered under normal conditions in either genotype. In addition to genes involved in pathways classically associated with reward, this gene set shows a striking enrichment in transcription factors that are specifically known for their involvement in brain development. Even more interestingly, as the authors demonstrate, several of these genes are expressed in neurogenic domains of the adult fish brain these are domains where neurons are generated from neural stem cells during adulthood. According to the researchers, " These factors, which are also dramatically down-regulated by amphetamine, can serve as valuable new entry points into studying the link between adult neurogenesis and addiction".

These results identify a new network of coordinated gene regulation that influences the response to amphetamine and may underlie the susceptibility to addiction.


'/>"/>

Contact: Graeme Baldwin
graeme.baldwin@biomedcentral.com
44-020-319-22165
BioMed Central
Source:Eurekalert

Related biology news :

1. Pitt team finds molecule that regulates heart size by using zebrafish screening model
2. Zebrafish provide a model for cancerous melanoma in humans
3. Alzheimer cell death in Zebrafish: Demise of neurons observed live for the first time
4. AZTI-Tecnalia coordinates a platform promoting the use of zebrafish in scientific research
5. NIH grants Phylonix Phase II SBIR to develop high-throughput in vivo zebrafish assays
6. Digital zebrafish embryo provides the first complete developmental blueprint of a vertebrate
7. Deadly genetic disease prevented before birth in zebrafish
8. A screening strategy using zebrafish targets genes that protect against hearing loss
9. Study shows how the zebrafish gets his stripe
10. Zebrafish to shed light on human mitochondrial diseases
11. Structure of protective protein in the eye lens revealed
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/3/2016)... , March 3, 2016  FlexTech, a SEMI ... categories of Innovation, Research & Development, Leadership in Education, ... This is the 9 th year of the ... of companies and individuals from past years . ... on a pre-described set of criteria, by a panel ...
(Date:3/3/2016)... SOTO, Kansas , March 3, 2016 ... Oncimmune,s Early CDT®-Lung, a blood test to aid ... cancer Early CDT®-Lung test to its clients ... Early CDT®-Lung test to its clients which include ... a leader in early cancer detection, today announced a ...
(Date:3/2/2016)... March 2, 2016 ... the "Global Biometrics as a Service ... --> http://www.researchandmarkets.com/research/cmt3hk/global_biometrics ) has ... Biometrics as a Service Market 2016-2020" ... Research and Markets ( http://www.researchandmarkets.com/research/cmt3hk/global_biometrics ) ...
Breaking Biology News(10 mins):
(Date:4/28/2016)... ... April 28, 2016 , ... ... will deliver a talk on its first-in-class technologies for tissue stem cell counting ... on RNAiMicroRNA Biology to Reprogramming & CRISPR-based Genome Engineering in Burlington, Massachusetts. ...
(Date:4/27/2016)... , ... April 27, 2016 , ... Cambridge Semantics, ... semantic web technology, today announced that it has been named to The Silicon Review’s ... financial services and other markets, Cambridge Semantics serves the needs of end users facing ...
(Date:4/27/2016)... (PRWEB) , ... April 27, 2016 , ... ... Touch screen mobile devices with fingerprint recognition for secure access, voice recognition ... only a few ways consumers are interacting with biometrics technology today. But ...
(Date:4/27/2016)... British Virgin Islands (PRWEB) , ... April 27, ... ... Holding Company Ltd. (d/b/a Biohaven) is pleased to announce the appointment of John Tilton ... he was an Executive Director and one of the founding commercial leaders responsible ...
Breaking Biology Technology: