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Disease knowledge may advance faster with CRISPR gene probing tool
Date:3/7/2013

ert any specific RNA partner molecule. The selected RNA serves as an adaptor that determines the target anywhere within the genome. "Targeting the machinery to new sites is extremely flexible and quick," Qi said.

The research team was able to get the system to work in mammalian cells as well as bacterial cells, and is working to improve its efficiency in mammalian cells, including human cells. The team aims to couple the Cas9 chassis to an enzyme that will enable the technology to turn genes on as well as off.

Such a versatile tool could prove valuable in efforts to reprogram cells for regenerative medicine. Lim's own lab is working on reprogramming immune cells to treat cancer.

"The idea is to reprogram cells to do the things we want them to do," Lim said. "We are still unlocking the secrets of the genome to harness the power of cellular reprogramming."


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Contact: Jeffrey Norris
jeff.norris@ucsf.edu
415-502-6397
University of California - San Francisco
Source:Eurekalert  

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