Navigation Links
Discovering cell surface proteins' behavior
Date:2/12/2013

A Simon Fraser University chemist is the lead author on a new paper that advances scientific understanding of the structure and function of glycoproteins, in particular the number and positioning of sugars on them. PLOS ONE, an international, peer-reviewed, open-access, online, scientific research journal, has just published the paper, N-glycoproteome of E14.Tg2a Mouse Embryonic Stem Cells.

Glycoproteins are membrane proteins and are often involved in human diseases. They facilitate communication between cells, and interactions with pathogens, such as viruses and bacteria, and communication with external environments.

SFU chemist Bingyun Sun and her colleagues have discovered how nature can vary the amount of a dominant sugar type (N-Glycan) on membrane proteins on a cell surface. The variation helps stabilize these proteins and facilitate their functioning. The researchers verified their observation of a correlation between the number of sugars on a glycoprotein and its function in five animal species worms, flies, fish, mice and humans. This led to their realization that the correlation has been conserved through evolution.

To obtain the number of N-Glycans on proteins, the researchers used proteomics a combination of mass spectrometry (MS) and high-performance liquid chromatography (HPLC). In less than an hour, high-throughput technique identifies the exact place where N-Glycans attach on hundreds of glycoproteins.

The scientists analyzed cell surface glycoproteins in one type of mouse embryonic stem cells by genetically shutting down the Hypoxanthine Phosphoribosyltransferase (HPRT) gene in the cells. As an aside, HPRT deficiency causes Lesch-Nyhan syndrome in humans, a metabolic disorder characterized by mental retardation and self-mutilation.

Sun says this deeper understanding of the correlation between sugars' positioning on glycoproteins and the proteins' functions will benefit medical researchers and the pharmaceutica
'/>"/>

Contact: Carol Thorbes
cthorbes@sfu.ca
778-782-3035
Simon Fraser University
Source:Eurekalert

Page: 1 2

Related biology news :

1. KIT: Processes at the surface of catalysts
2. NASA satellite measurements imply Texas wind farm impact on surface temperature
3. Secret soil cracks linger, despite surface sealing
4. Proteomic analysis of immunocamouflaged surfaces
5. The most contaminated surfaces in hotel rooms
6. Wrinkled surfaces could have widespread applications
7. Sea surface temperatures reach record highs on Northeast continental shelf
8. Professor publishes on first-ever imaging of cells growing on spherical surfaces
9. 3dMD Transitions Anatomical Research from 3D-Static to 4D-Movement Surface Imaging
10. Ancient microbes found living beneath the icy surface of Antarctic lake
11. Surface analysis techniques for advanced materials enhance Mazovias research potential
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:11/18/2014)... 17, 2014 The Parenteral Drug Association (PDA) today ... will speak and at least seven more will participate in ... Omni Shoreham Hotel in Washington D.C. , ... have significant support from the regulatory agencies in ... in our effort to help advance the use of ...
(Date:11/10/2014)... detergent pods began appearing on U.S. store shelves in ... numbers ever since. The small packets can be tossed ... out a liquid or powder. The convenience, though, has ... study from researchers at Nationwide Children,s Hospital found that ... reports of 17,230 children younger than 6 years of ...
(Date:11/6/2014)... deaths occur because of metastasis, yet progress in ... slow. , "It,s been particularly challenging to design ... research fellow in systems biology at Harvard Medical ... until after they,ve already metastasized." , Gujral and ... should help researchers better understand how metastasis begins. ...
Breaking Biology News(10 mins):FDA's Janet Woodcock, EMA's Emer Cooke Headline PDA Quality Metrics Conference 2Study finds laundry detergent pods, serious poisoning risk for children 2Migration negation 2Migration negation 3
... medical woes ranging from cancer to bird flu will ... The newly-patented device will offer small and medium-sized researchers ... It will also allow them a chance, at last, ... analyze drugs and view the characteristics of protein. ...
... a gene known to increase the risk of Alzheimer,s ... Oregon Health & Science University study has found. ... that 7- to 10-year-olds with a member of a ... and neuroprotection display reduced spatial learning and memory, associated ...
... include: continental deformation and the San Andreas fault; ... between Britain and America; environmental stress and the ... Canada,s Mackenzie River basin as a CO2 source ... the absence of active volcanism; and geologic evolution ...
Cached Biology News:Council to honor NJIT SmartPin 2Children with gene show reduced cognitive function 2November GEOLOGY and GSA TODAY Media Highlights 2November GEOLOGY and GSA TODAY Media Highlights 3November GEOLOGY and GSA TODAY Media Highlights 4November GEOLOGY and GSA TODAY Media Highlights 5November GEOLOGY and GSA TODAY Media Highlights 6November GEOLOGY and GSA TODAY Media Highlights 7November GEOLOGY and GSA TODAY Media Highlights 8November GEOLOGY and GSA TODAY Media Highlights 9November GEOLOGY and GSA TODAY Media Highlights 10
(Date:11/26/2014)... November 26, 2014 On January, 14, ... Baltimore and Washington D.C. science community to the UMBC ... event, sponsored by the Protein Society, will take place ... Center, Room 312. Through discussion, presentations and workshops, ... importance of proteins, their role in the industry and ...
(Date:11/26/2014)... NJ (PRWEB) November 25, 2014 ... has officially joined the Morris County (New Jersey) ... which is a leading business organization in the ... opportunity to further engage some key clients in ... operations in the geographic area. Membership enables Whitehouse ...
(Date:11/26/2014)... Continuing its award-winning streak, Nerium International, the leader ... for outstanding creative achievement by its marketing and communication ... organization’s top honor, in the Branding category for its ... of this year. The event showcased top speakers and ... awarded a Gold MarCom Award in the Special Events ...
(Date:11/26/2014)... November 25, 2014 Miles Holder, formerly ... the Graphel Carbon Products team as Sales/Marketing Manager. Mr. ... and customer service for the graphite industry. , “We ... our team,” stated Dave Trinkley, VP of Market and ... range of experience in the graphite industry, along with ...
Breaking Biology Technology:Whitehouse Laboratories Joins Morris County Chamber of Commerce 2Whitehouse Laboratories Joins Morris County Chamber of Commerce 3Nerium International™ Wins One Platinum, Two Gold MarCom Awards 2Miles Holder Joins Graphel Corporation as Sales/Marketing Manager 2
... required regulatory approvals for the $2.3 billion acquisition of ... divest of two divisions of the company they are ... manufacturer plans to integrate Instrumentarium it into its ... CEO, Dow Wilson.. , ,The final required ...
... to open a new state-of-art healthcare facility in October, ... latest in digital imaging technology from technology vendor Emageon. ... and distribution of digital medical images across its integrated ... in 1984 is a nationally recognized leader in efforts ...
... Internet connection? , ,MADISON, WI - McDonalds ... connections in five Milwaukee locations as part of a ... restaurants in the San Francisco Bay Area, Metropolitan New ... McDonald's Wireless Internet service. , ,Customers can purchase an ...
Cached Biology Technology:Aurora Selects New Imaging Technology 2Wisconsin McDonalds Go Wireless 2