Navigation Links
Discovering cell surface proteins' behavior
Date:2/12/2013

A Simon Fraser University chemist is the lead author on a new paper that advances scientific understanding of the structure and function of glycoproteins, in particular the number and positioning of sugars on them. PLOS ONE, an international, peer-reviewed, open-access, online, scientific research journal, has just published the paper, N-glycoproteome of E14.Tg2a Mouse Embryonic Stem Cells.

Glycoproteins are membrane proteins and are often involved in human diseases. They facilitate communication between cells, and interactions with pathogens, such as viruses and bacteria, and communication with external environments.

SFU chemist Bingyun Sun and her colleagues have discovered how nature can vary the amount of a dominant sugar type (N-Glycan) on membrane proteins on a cell surface. The variation helps stabilize these proteins and facilitate their functioning. The researchers verified their observation of a correlation between the number of sugars on a glycoprotein and its function in five animal species worms, flies, fish, mice and humans. This led to their realization that the correlation has been conserved through evolution.

To obtain the number of N-Glycans on proteins, the researchers used proteomics a combination of mass spectrometry (MS) and high-performance liquid chromatography (HPLC). In less than an hour, high-throughput technique identifies the exact place where N-Glycans attach on hundreds of glycoproteins.

The scientists analyzed cell surface glycoproteins in one type of mouse embryonic stem cells by genetically shutting down the Hypoxanthine Phosphoribosyltransferase (HPRT) gene in the cells. As an aside, HPRT deficiency causes Lesch-Nyhan syndrome in humans, a metabolic disorder characterized by mental retardation and self-mutilation.

Sun says this deeper understanding of the correlation between sugars' positioning on glycoproteins and the proteins' functions will benefit medical researchers and the pharmaceutica
'/>"/>

Contact: Carol Thorbes
cthorbes@sfu.ca
778-782-3035
Simon Fraser University
Source:Eurekalert

Page: 1 2

Related biology news :

1. KIT: Processes at the surface of catalysts
2. NASA satellite measurements imply Texas wind farm impact on surface temperature
3. Secret soil cracks linger, despite surface sealing
4. Proteomic analysis of immunocamouflaged surfaces
5. The most contaminated surfaces in hotel rooms
6. Wrinkled surfaces could have widespread applications
7. Sea surface temperatures reach record highs on Northeast continental shelf
8. Professor publishes on first-ever imaging of cells growing on spherical surfaces
9. 3dMD Transitions Anatomical Research from 3D-Static to 4D-Movement Surface Imaging
10. Ancient microbes found living beneath the icy surface of Antarctic lake
11. Surface analysis techniques for advanced materials enhance Mazovias research potential
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:12/22/2014)... 22, 2014  NXT-ID, Inc. (NASDAQ: NXTD and ... on the growing mobile commerce market, announces it has retained ... for the 2015 International CES debut of the Wocket™ biometric ... be at the NXT-ID booth January 6th and 7 th ...
(Date:12/19/2014)... and Markets ( http://www.researchandmarkets.com/research/86ncd6/micro_market ) has announced the ... Security Systems Market" report to their offering. ... perimeter security market is estimated to grow at a CAGR ... holds a larger share in this market, Canada ...
(Date:12/17/2014)... , Dec. 15, 2014 The Defense Logistics Agency ... detect and prevent counterfeit microcircuits from entering into its ... an in-house microcircuit anti-counterfeit initiative dubbed DNA marking. The ... increasing their reliability throughout the supply chain. The new ...
Breaking Biology News(10 mins):NXT-ID Hires Master Pickpocket and Security Consultant Apollo Robbins for Wocket Smart Wallet CES 2015 Debut 2NXT-ID Hires Master Pickpocket and Security Consultant Apollo Robbins for Wocket Smart Wallet CES 2015 Debut 3NXT-ID Hires Master Pickpocket and Security Consultant Apollo Robbins for Wocket Smart Wallet CES 2015 Debut 4Micro Market Monitor: North America Perimeter Security Systems Market (Intrusion Detection Sensor, Video Surveillance, Communication/Alarm and Notification, Access Control System) 2Micro Market Monitor: North America Perimeter Security Systems Market (Intrusion Detection Sensor, Video Surveillance, Communication/Alarm and Notification, Access Control System) 3Defense Logistics Agency Launches DNA Marking Capability to Strengthen Microcircuit Supply Chain 2Defense Logistics Agency Launches DNA Marking Capability to Strengthen Microcircuit Supply Chain 3
... Salk Institute Laboratory of Genetics and holder of the ... Neurodegenerative Diseases, has been named a 2011 recipient of ... Research Projects (T-R01) program. Only 17 T-R01 projects ... with a total of $15.9 million in funding. Gage, ...
... has been published in the journal Coral Reefs by Giacomo Bernardi, ... Santa Cruz. In the video, an orange-dotted tuskfish digs ... rock, and repeatedly throws the clam against the rock to crush ... "What the movie shows is very interesting. The animal excavates sand ...
... be linked to the decline of water calcium levels ... that helps keep blooms at bay. Daphniaalso known ... on algae and keeping it in check. However, without ... water calcium levels get low and Daphnia populations decrease ...
Cached Biology News:Video shows tool use by a fish 2Loss of 'lake lawnmowers' leads to algae blooms 2
(Date:1/22/2015)... Eckert & Ziegler Vitalea Science (EZVS), ... to the pharmaceutical and biotech industry, has announced that ... the position of Director of Science & Technology. , ... experience in drug metabolism and bioanalysis to EZVS in ...
(Date:1/22/2015)... | RBJ today announces the firm brokered a long-term lease ... leading biopharmaceutical company, at Two Ledgemont Center in ... , president, and Brian Cohen , senior vice president, ... at 95 Hayden Ave. Photo - http://photos.prnewswire.com/prnh/20150122/170765 ...
(Date:1/22/2015)... | RBJ today announces the firm brokered a long-term lease ... leading biopharmaceutical company, at Two Ledgemont Center in ... , president, and Brian Cohen , senior vice president, ... at 95 Hayden Ave. Photo - http://photos.prnewswire.com/prnh/20150122/170765 ...
(Date:1/22/2015)... 2015 The laboratory information management systems ... a number of technological advancements due to factors such ... need to integrate healthcare systems, and increasing government support ... system integration. Key players in the market focus on ...
Breaking Biology Technology:Eckert & Ziegler Vitalea Science appoints Mark Seymour, PhD as Director of Science & Technology 2Eckert & Ziegler Vitalea Science appoints Mark Seymour, PhD as Director of Science & Technology 3Transwestern | RBJ Advises Shire in 202,000 SF Lease, Creating Boston's Largest Suburban Biotech Campus 2Transwestern?RBJ Advises Shire in 202,000 SF Lease, Creating Boston's Largest Suburban Biotech Campus 2Laboratory Information Management Systems Market is Expected to Reach $1,323.6 Million by 2019 - New Research Report by MarketsandMarkets 2Laboratory Information Management Systems Market is Expected to Reach $1,323.6 Million by 2019 - New Research Report by MarketsandMarkets 3Laboratory Information Management Systems Market is Expected to Reach $1,323.6 Million by 2019 - New Research Report by MarketsandMarkets 4
... IRVINE, Calif., Jan. 18, 2011 ... that a new clinical study demonstrating the clinical accuracy of ... presented last week at the Society for Technology in ... by the Johns Hopkins School of Medicine, evaluated the accuracy ...
... 2011 BioStorage Technologies, a global leader in ... storage for the bioscience industry, today introduced the ... sample management system --  ISISS®. This latest release ... intuitive sample tracking that allows for increased control ...
... ERIE, Pa., Jan. 13, 2011 Kanzius Cancer Research ... at The University of Texas M.D. Anderson Cancer Center ... the American Association of Cancer Research,s Clinical Cancer ... A. Curley and Evan S. Glazer,s studies prove that ...
Cached Biology Technology:New Johns Hopkins School of Medicine Study Shows Masimo Noninvasive Hemoglobin Monitoring is Accurate During Complex Spine Surgeries 2New Johns Hopkins School of Medicine Study Shows Masimo Noninvasive Hemoglobin Monitoring is Accurate During Complex Spine Surgeries 3New Johns Hopkins School of Medicine Study Shows Masimo Noninvasive Hemoglobin Monitoring is Accurate During Complex Spine Surgeries 4New Johns Hopkins School of Medicine Study Shows Masimo Noninvasive Hemoglobin Monitoring is Accurate During Complex Spine Surgeries 5New Johns Hopkins School of Medicine Study Shows Masimo Noninvasive Hemoglobin Monitoring is Accurate During Complex Spine Surgeries 6BioStorage Technologies Introduces New Version of Web-based Tracking and Sample Management System - ISISSĀ® 2Targeted Gold Nanoparticles and Noninvasive Radio Fields Attacking One of the Deadliest and Least Curable Types of Cancer: Pancreatic Carcinoma 2
... are cytopathic effect inhibition assays, also known as ... tissue culture supernatents; serum from PK studies and ... is defined as the quantity of interferon required ... viral infection by 50%. All standards ...
... Topoisomerase I from vaccinia virus is a ... positive and negative superhelical turns (also called ... DNA. The product of the reaction is ... positive or negative superhelical turns. DNA Topoisomerase ...
... Topoisomerase I from vaccinia virus is ... both positive and negative superhelical turns (also ... closed DNA. The product of the reaction ... fewer positive or negative superhelical turns. DNA ...
... CpGenome Fast DNA Modification Kit contains reagents ... a DNA sample. In the bisulfite reaction, ... converting them to uracils, while 5-methylcytosines remain ... DNA will differ depending on whether the ...
Biology Products: