Navigation Links
Digital zebrafish embryo provides the first complete developmental blueprint of a vertebrate
Date:10/9/2008

Researchers at the European Molecular Biology Laboratory (EMBL) have generated a digital zebrafish embryo - the first complete developmental blueprint of a vertebrate. With a newly developed microscope scientists could for the first time track all cells for the first 24 hours in the life of a zebrafish. The data was reconstructed into a three-dimensional, digital representation of the embryo. The study, published in the current online issue of Science, grants many new insights into embryonic development. Movies of the digital embryo and the underlying database of millions of cell positions, divisions and tracks will be made publicly available to provide a novel resource for research and scientific training.

To get from one cell to a complex organism, cells have to divide, travel around the body and arrange intricate shapes and specialized tissues. The best way to understand these dynamic processes is to look at what happens in the first few hours of life in every part of an embryo. While this is possible with invertebrates with a few hundred cells, like worms for example, it has so far been impossible to achieve for vertebrates.

"Imagine following all inhabitants of a town over the course of one day using a telescope in space. This comes close to tracking the 10 thousands of cells that make up a vertebrate embryo only that the cells move in three dimensions," says Philipp Keller. Together with Annette Schmidt he carried out the research in the labs of Jochen Wittbrodt and Ernst Stelzer at EMBL.

Two newly developed technologies were key to the scientists' interdisciplinary approach to tracking a living zebrafish embryo from the single cell stage to 20,000 cells: a Digital Scanned Laser Light Sheet Microscope, that scans a living organism with a sheet of light along many different directions so that the computer can assemble a complete 3D image, and a large-scale computing pipeline operated at the Karlsruhe Institute of Technology.

Zebrafish is a widely used model organism that shares many features with higher vertebrates. Taking more than 400,000 images per embryo the interdisciplinary team generated terabytes of data on cell positions, movements and divisions that were reassembled into a digital 3D representation of the complete developing embryo.

"The digital embryo is like Google EarthTM for embryonic development. It gives an overview of everything that happens in the first 24 hours and allows you to zoom in on all cellular and even subcellular details," says Jochen Wittbrodt, who has recently moved from EMBL to the University of Heidelberg and the Karlsruhe Institute of Technology.

New insights provided by the digital embryo include: fundamental cell movements that later on form the heart and other organs are different than previously thought and the position of the head-tail body axes of the zebrafish is induced early on by signals deposited in the egg by the mother.

The new microscopy technology is also applicable to mice, chickens and frogs. A comparison of digital embryos of these species is likely to provide crucial insights into basic developmental principles and their conservation during evolution.


'/>"/>

Contact: Anna-Lynn Wegener
wegener@embl.de
49-622-138-7452
European Molecular Biology Laboratory
Source:Eurekalert  

Related biology news :

1. Entrust Digital Signatures Help Secure More Than 18 Million U.S. ePassports
2. Book is the first high-resolution digital mouse brain atlas designed for Web applications
3. FEBS Letters Structured Digital Abstracts experiment
4. Vobile Launches VideoDNA Live; Enables Real-Time Digital Content Protection for Live Broadcast Events
5. Fixing the education digital disconnect one video game at a time
6. Digital cameras, remote satellites measure crop water demand
7. Zebrafish to shed light on human mitochondrial diseases
8. Study shows how the zebrafish gets his stripe
9. A screening strategy using zebrafish targets genes that protect against hearing loss
10. Deadly genetic disease prevented before birth in zebrafish
11. Scientists identify embryonic stem cells by appearance alone
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Digital zebrafish embryo provides the first complete developmental blueprint of a vertebrate
(Date:3/31/2016)... RATON, Florida , March 31, 2016 ... LEGX ) ("LegacyXChange" or the "Company") ... for potential users of its soon to be launched ... video ( https://www.youtube.com/channel/UCyTLBzmZogV1y2D6bDkBX5g ) will also provide ... the use of DNA technology to an industry that ...
(Date:3/29/2016)... , March 29, 2016 ... "Company") LegacyXChange "LEGX" and SelectaDNA/CSI Protect are pleased to ... ink used in a variety of writing instruments, ensuring ... of originally created collectibles from athletes on LegacyXChange will ... analysis of the DNA. Bill Bollander ...
(Date:3/22/2016)... 2016 According to ... for Consumer Industry by Type (Image, Motion, Pressure, ... & IT, Entertainment, Home Appliances, & Wearable ... 2022", published by MarketsandMarkets, the market for ... USD 26.76 Billion by 2022, at a ...
Breaking Biology News(10 mins):
(Date:5/25/2016)... ... May 25, 2016 , ... Thailand’s Board ... BIO 2016 in San Francisco. Located at booth number 7301, representatives from the ... questions and discuss the Thai biotechnology and life sciences sector. , Deputy ...
(Date:5/25/2016)... (PRWEB) , ... May 25, 2016 , ... Scientists at ... line options being tried for mesothelioma may be hampering the research that could lead ... research. Click here to read it now. , The team evaluated 98 ...
(Date:5/24/2016)... ... May 24, 2016 , ... Cell ... injuries, will be accelerated by research at Worcester Polytechnic Institute (WPI) that yielded ... healing and tissue regeneration. , The novel method, developed by WPI faculty members ...
(Date:5/23/2016)... ... May 23, 2016 , ... The need for blood donations in South Texas and ... the South Texas Blood & Tissue Center, blood donations are on the decline. In fact, ... they are down 21 percent in South Texas in the last four years alone. , ...
Breaking Biology Technology: