Navigation Links
Development of a FRET sensor for real-time imaging of intracellular redox dynamics
Date:6/7/2011

ation between the redox states of the probe in complex biological specimens. Furthermore, the highly oxidative midpoint potential of the novel probe is ideal for measuring glutathione redox potentials in oxidative compartments of mammalian cells."

Recently, a different innovative ratiometric probe - a redox-sensitive GFP (roGFP) - has been developed in another lab. The measurement with the roGFP sensor involves the ratio of intensities of two sequential images, acquired at two different excitation wavelengths. Two thiol groups form/break a disulfide bond that modulates the peak excitation wavelength of the roGFP chromophore in response to the redox environment. Bryan Spring, a co-author, notes, "The roGFP and the FRET-based sensors have contrasting characteristics. The FRET-based sensor may prove advantageous for intravital microscopy studies, because only a single laser line is required. In contrast, roGFP requires sequential scanning of two laser lines, which slows the frame rate of image acquisition; also, the images must be compensated for the different laser intensities in order to correct for wavelength-dependent tissue scattering, and the measurement relies on the optical alignment of two excitation light beams. However, the roGFP probe is sensitive to a different range of oxidation-reduction potentials than our FRET probe, possibly leading to complementary applications." Spring adds, "We look forward to further exciting innovations for optimizing the performance of oxidation-reduction-based sensors."

Dr. Rex Gaskins, who led the project remarked, "Distinct advantages of the FRET-based approach include: (1) the ability to quantify the change in redox state; (2) independence of sensor concentration; and (3) modularity, the ability to precisely tune the redox sensitivity and range by exchange of the switch or the fluorophore modules in the probe. We expect that newly developed redox-sensitive probes could potentially be critical to a better
'/>"/>

Contact: Vladimir L. Kolossov
viadimer@illinois.edu
Society for Experimental Biology and Medicine
Source:Eurekalert

0

GOOD

Page: 1 2 3

Related biology news :

1. Developmental disease is recreated in an adult model
2. Ulcer bacteria may contribute to development of Parkinsons disease
3. A study will enable the survival and growth of the 3 development phases of the European eel
4. Building brains: An introduction to neural development
5. Gene variant linked with development of COPD in men
6. Scientists find new class of compounds with great potential for research and drug development
7. New organic catalyst should enhance drug research and development
8. Brain development goes off track as vulnerable individuals develop schizophrenia
9. Study: Pace of brain development still strong in late teens
10. Spikemoss genome offers new paths for biofuels research -- bridges plant development gap
11. Brain cell migration during normal development may offer insight on how cancer cells spread
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:5/24/2013)... system to combat metastatic cancer , Cancer cells ... the immune system. Stimulation of the immune system can ... factors that cause the immune system to ignore cancer ... to suppress the immune system response. In this issue ... led by Ronald Levy at Stanford University found that ...
(Date:5/24/2013)... scientists from around the world today issued a stark ... of one or two generations, the majority of the ... the handicap of severe pressure on fresh water, an ... substitute. This handicap will be self-inflicted and is, we ... chronic underlying problems led by mismanagement and sent a ...
(Date:5/23/2013)... at Rollins School of Public Health at Emory ... of Technology, have received a $4 million grant ... at Emory University (Health and Exposome Research Center: ... exposome-based center grant awarded in the United States. ... the National Institute of Environmental Health Sciences (NIEHS) ...
Breaking Biology News(10 mins):JCI early table of contents for May 24, 2013 2JCI early table of contents for May 24, 2013 3JCI early table of contents for May 24, 2013 4JCI early table of contents for May 24, 2013 5JCI early table of contents for May 24, 2013 6JCI early table of contents for May 24, 2013 7JCI early table of contents for May 24, 2013 8A majority on Earth face severe self-inflicted water woes within 2 generations: Scientists 2A majority on Earth face severe self-inflicted water woes within 2 generations: Scientists 3A majority on Earth face severe self-inflicted water woes within 2 generations: Scientists 4Emory, Georgia Tech receive first human exposome center grant in US 2Emory, Georgia Tech receive first human exposome center grant in US 3
... you,re a dieter. Chronic dieters tend to ... according to a new study in the Journal ... M. Nowlis, Naomi Mandel, and Andrea C. Morales (all ... food packages that are marketed to help people control ...
... deceivingeven in life,s tiniest critters. From first detection in ... its recent characterization by the U.S. Department of Energy Joint ... adhaerens, appears to harbor a far more complex suite of ... August 21 online edition of the journal Nature, ...
... Scientists at The University of Manchester have developed a ... technology that could lead to quick testing for serious ... of new drugs. Researchers working at the Manchester ... unveiled a new technique for producing functional ,protein chips, ...
Cached Biology News:M&Ms as diet food? 100-calorie pack misconceptions 2Genome of simplest animal reveals ancient lineage, confounding array of complex capabilities 2Genome of simplest animal reveals ancient lineage, confounding array of complex capabilities 3Chips are down as Manchester makes protein scanning breakthrough 2
(Date:5/24/2013)... Toronto, Canada (PRWEB) May 24, 2013 ... selective barriers, and uptake or efflux transporters at the ... may be related to unbound fractions in the tissue ... understanding of these disposition challenges is required to guide ... and to identify any species-dependent variables that may impact ...
(Date:5/24/2013)... Vestiage, Inc. (stock symbol "VEST") a publicly traded ... development, sales and marketing of premium branded highly ... it is awarding exclusive Vestiage territories in the ... Illuminating Facial Care. Qualified applicants can request an ... to info(at)vestiageinc(dot)com and putting RELUMA in the "Subject" ...
(Date:5/23/2013)... , May 24, 2013 Die ... – Bei der englischen Version ... , 91 % der Teilnehmer einer groß angelegten Asthmaumfrage ... % laut GINA [*] -Definition [1] als kontrolliert ... mit Asthma generell einer von vier Denkweisen zugeordnet werden ...
(Date:5/23/2013)... 23, 2013 Venaxis, Inc. (Nasdaq:   APPY), ... obtaining FDA clearance and commercializing its rapid, protein biomarker-based ... of an underwritten public offering of 10,000,000 shares of ... shares of its common stock at an exercise price ... price of $1.25 per share and related warrant.  Venaxis ...
Breaking Biology Technology:DMPK for Targeted Tissue Delivery: Solutions for the Most Challenging Part of Outsourcing, New Life Science Webinar Hosted by Xtalks 2Vestiage Announces Launch of Exclusive Territories for Reluma Brand Sales in USA 2Vestiage Announces Launch of Exclusive Territories for Reluma Brand Sales in USA 3Vestiage Announces Launch of Exclusive Territories for Reluma Brand Sales in USA 4Vestiage Announces Launch of Exclusive Territories for Reluma Brand Sales in USA 5Neue Forschungsergebnisse zeigen Verbindung zwischen suboptimaler Asthmaversorgung und Patienteneinstellungen in Europa auf 2Neue Forschungsergebnisse zeigen Verbindung zwischen suboptimaler Asthmaversorgung und Patienteneinstellungen in Europa auf 3Neue Forschungsergebnisse zeigen Verbindung zwischen suboptimaler Asthmaversorgung und Patienteneinstellungen in Europa auf 4Neue Forschungsergebnisse zeigen Verbindung zwischen suboptimaler Asthmaversorgung und Patienteneinstellungen in Europa auf 5Neue Forschungsergebnisse zeigen Verbindung zwischen suboptimaler Asthmaversorgung und Patienteneinstellungen in Europa auf 6Neue Forschungsergebnisse zeigen Verbindung zwischen suboptimaler Asthmaversorgung und Patienteneinstellungen in Europa auf 7Venaxis Announces Pricing of Offering of Common Stock and Warrants 2Venaxis Announces Pricing of Offering of Common Stock and Warrants 3
... /PRNewswire-FirstCall/ - Nventa,Biopharmaceuticals Corporation (TSX: NVN) today ... initiated dosing of the second cohort of,patients ... examining the safety of its,lead candidate, HspE7, ... to,cervical cancer. HspE7 is an investigational therapeutic ...
... there has been growing interest in the question, if ... via manure application is of environmental relevance. In a ... journal CLEAN, Kreuzig and co-workers now focused on the ... to the so far available data set on fate ...
... Dec. 5 The American Society of,Hematology (ASH), ... more than 20,000 attendees at the 49th ASH ... World Congress Center in,Atlanta. The meeting will premiere ... "The ASH annual meeting is the premier forum ...
Cached Biology Technology:Nventa completes enrollment and initial dosing of second cohort in Phase 1 HspE7 safety trial 2Nventa completes enrollment and initial dosing of second cohort in Phase 1 HspE7 safety trial 3Nventa completes enrollment and initial dosing of second cohort in Phase 1 HspE7 safety trial 4Nventa completes enrollment and initial dosing of second cohort in Phase 1 HspE7 safety trial 5New insights into the fate of anti-parasitics in manure and manured soils 249th Annual Meeting of the American Society of Hematology Will Feature the Latest Research in Blood Diseases 249th Annual Meeting of the American Society of Hematology Will Feature the Latest Research in Blood Diseases 3
... ZMD.368. Immunogen: Synthetic peptide derived ... human WNT2B/WNT13 protein. Specificity: Specific ... and rat WNT2B/WNT13 proteins. Recognizes both ... Reactivity: Mouse Human Rat (positive controls: ...
Immunogen: Heat inactivated bacterial cells of Salmonella enteriditis,paratyphi and typhimurium Storage: 4 C...
Immunogen: Secretory component isolated from human colostrum. Storage: 4 C...
... I-96 Plate can be used either in ... large-scale (i.e., 96-well) purification of DNA and/or ... unique silica-based matrix make it perfect for ... RNA in = 10 l eluate per ...
Biology Products: