Navigation Links
Development of a FRET sensor for real-time imaging of intracellular redox dynamics
Date:6/7/2011

In work published in the June 2011 issue of Experimental Biology and Medicine, Kolossov, Spring and their co-investigators - a multidisciplinary team within the Institute for Genomic Biology at the University of Illinois - have transferred the concept of redox-sensitive Green Fluorescent Proteins (GFPs) to a quantitative Frster resonance energy transfer (FRET) imaging platform. For the FRET-based sensors, a change in redox induces a conformational change in a redox-sensitive switch that links two fluorescent proteins (the donor and acceptor), changing their distance, which in turn causes a detectable change in FRET efficiency. In its oxidized state the wavelength spectrum of the sensor's fluorescence emission is red-shifted (due to increased acceptor fluorescence), independent of variations in the local sensor concentration or in the intensity of the excitation light. As explained by Robert Clegg, a pioneer in the development of novel applications of optical microscopy in the biological sciences and key collaborator on the study, "FRET-based sensors circumvent the complications associated with imaging methods based on fluorescence intensity, since the increase in the FRET acceptor molecule's fluorescence can only take place if there is a change in the efficiency of energy transfer. This specific and discriminatory feature of FRET is one of the driving motives behind our development of a FRET-based assay rather than relying only on changes in the fluorescent intensity of a single component."

The current publication builds on the authors' previous work, where they reported a series of first-generation redox-sensitive linkers flanked by FRET donor and acceptor GFP-variants. As summarized by co-author Vladimir Kolossov, "The major advance in the current study is an improved dynamic range of the spectroscopic signal; in other words, a greater difference between fully reduced and oxidized states. Increasing the dynamic range leads to better discrimin
'/>"/>

Contact: Vladimir L. Kolossov
viadimer@illinois.edu
Society for Experimental Biology and Medicine
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Developmental disease is recreated in an adult model
2. Ulcer bacteria may contribute to development of Parkinsons disease
3. A study will enable the survival and growth of the 3 development phases of the European eel
4. Building brains: An introduction to neural development
5. Gene variant linked with development of COPD in men
6. Scientists find new class of compounds with great potential for research and drug development
7. New organic catalyst should enhance drug research and development
8. Brain development goes off track as vulnerable individuals develop schizophrenia
9. Study: Pace of brain development still strong in late teens
10. Spikemoss genome offers new paths for biofuels research -- bridges plant development gap
11. Brain cell migration during normal development may offer insight on how cancer cells spread
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:7/10/2014)... from The University of Texas Health Science Center at ... (IYG), a health education program designed to delay sexual ... dating violence behaviors among minority youth. , According to ... of high school youth are victims of physical dating ... percent are victims of emotional dating violence. Previous studies ...
(Date:7/10/2014)... children and teens living with a cardiac pacemaker, a ... quality of life, reports a study in the July ... , the official journal of the Society for ... published by Lippincott Williams & Wilkins , a ... may function as a protective factor against lower health-related ...
(Date:7/10/2014)... National Cancer Research Centre (CNIO) Macromolecular Crystallography Group ... protein called BuD to DNA in order to ... the researcher who led the study, says the ... the instructions contained in the genome to treat ... study is published in the journal Acta ...
Breaking Biology News(10 mins):It's Your Game ... Keep It Real reduces dating violence among minority youth 2For children with pacemakers, 'self-competence' affects quality of life 2CNIO scientists develop technology to redirect proteins towards specific areas of the genome 2
... Munich have developed a new method for visualizing material ... team led by Bert Nickel has, for the first ... organic thin-film solar cells using laser light for localized ... the scientific journal " Advanced Materials ". "We have ...
... has published research this week in a leading science ... they were raised rather than that likely to maximize ... of habitat selection theory. The paper is published ... on June 26 and includes co-authors Michael Palmer, ...
... significantly challenged as production levels fall due to ... the industry,s competitiveness are being sought. The South ... Resources and Energy (DMITRE) has invited VTT Technical ... study that identifies potential routes by which the ...
Cached Biology News:Organic electronics: Imaging defects in solar cells 2Bioeconomy as a solution for South Australia's declining forest industry 2Bioeconomy as a solution for South Australia's declining forest industry 3
(Date:1/15/2014)... January 15, 2014 Freeslate, Inc ... today announced that Lupin Limited, one of India’s top ... CM Protégé PharmD System for high throughput solid ... is focused on a wide range of quality, affordable ...
(Date:1/14/2014)... Histogen, Inc., a regenerative medicine company ... under simulated embryonic conditions, today announced that they have ... Inc. for physician-dispensed aesthetic products containing Histogen’s proprietary multipotent ... is an amendment to the existing license between Histogen ...
(Date:1/14/2014)... Md. , Jan. 14, 2014  Organovo announced today ... the National Institutes of Health (NIH) to help scientists ... treatments to patients on a faster timeline. Organovo is ... (NCATS) and the National Eye Institute (NEI) to develop ...
(Date:1/14/2014)... Jan. 14, 2014   Kinaxis ®, provider of RapidResponse ... sales and operations planning ( S&OP ) service, is proud ... , which will be held at the Hilton San Diego ... Kinaxis customer Elisabeth Kaszas , Director of Supply Chain ...
Breaking Biology Technology:Lupin Selects Freeslate’s CM Protégé PharmD System to Accelerate Polymorph Screening for Drug Development 2Lupin Selects Freeslate’s CM Protégé PharmD System to Accelerate Polymorph Screening for Drug Development 3Histogen and Suneva Medical Expand License for Cell Conditioned Media-based Aesthetic Products Internationally 2Histogen and Suneva Medical Expand License for Cell Conditioned Media-based Aesthetic Products Internationally 3Organovo Announces Collaboration with National Institutes of Health 2Organovo Announces Collaboration with National Institutes of Health 3Event Alert: Kinaxis Customer to Present at the Biomanufacturing Summit "Supply Chain: Improving Network Effectiveness" 2
... Encorium Group, Inc. (Nasdaq:  ENCO), (the "Company"), ... conducting studies in over 30 countries for many ... today announced that, on October 20, 2010, it ... (the "Panel") has determined to delist the Company,s ...
... Inc (OTC: OMCM.OB) today announced that it ranked number ... 500 of the fastest growing technology, media, telecommunications, life ... are based on the percentage of fiscal year revenue ... percent during this period. OmniComm,s CEO, Cornelis ...
... even harder in recent years the influenza A virus has ... Reporting their findings in the journal Science, researchers ... not, and how future treatments can defeat the nasty bug no ... are no longer recommended by the CDC for use against flu. ...
Cached Biology Technology:Encorium Announces NASDAQ Panel Decision to Delist Common Stock; Announces Changes to its Board of Directors 2Encorium Announces NASDAQ Panel Decision to Delist Common Stock; Announces Changes to its Board of Directors 3Encorium Announces NASDAQ Panel Decision to Delist Common Stock; Announces Changes to its Board of Directors 4OmniComm Ranks Number 220 on Deloitte's 2010 Technology Fast 500 for Fastest Growing Companies in North America 2OmniComm Ranks Number 220 on Deloitte's 2010 Technology Fast 500 for Fastest Growing Companies in North America 3Putting a bull's-eye on the flu: Detailing influenza's structure for drug targeting 2
...
... The Varian 500-MS LC Ion Trap ... high-throughput LC/MS analysis where sensitivity, reliability ... 500-MS provides a robust platform for ... mass resolution, and mass stability reflect ...
... modular benchtop fume hood ventilates ... safe indoor release of exhaust. ... organic contaminants. Configurations are available ... options include final HEPA filter, ...
... ideal modular cleanroom for biological safety ... require cleanliness (to Class 10/ISO 3) ... to create ultra-smooth surfaces that are ... One-inch radius corners simplify cleaning. Unique ...
Biology Products: