Navigation Links
Developing a tool for identification -- even using very degraded DNA samples
Date:4/12/2011

ence and of the sequential fragments of the ends that have remained trapped between the two primers. With the tool he has developed, Dr Odriozola has enhanced the design of the current primers. Thus, the sequential fragments that have remained at the extremes of the STR are shorter than those with conventional techniques (they are called miniSTR, because there is an approximation of the focus on the STR), and an identification can be obtained despite the DNA to be analysed being fragmented.

The more STRs from the same sample analysed, the greater the precision when determining to whom the sample belongs. With regard to this, Dr Odriozola has managed to develop sufficient tools (primers) to study 14 and 11 mini STRs, employing two kits that are mutually combinable. Besides developing these kits, he has shown in the process of validation that, effectively, identifications can be undertaken even with very degraded DNA samples. This fact is of great relevance given that, to date, rarely has it been possible to validate a tool of this kind, and the Forensic Genetics equipment must be totally reliable to be valid.

Methodology for looking for mutations

The primers cannot be situated in any zone whatsoever: it is fundamental that there is no mutation in the fragments that remain at the STR ends. That is, they cannot adhere to sequential fragments that vary according to the person (they would thereby lose information that could be useful for discriminating persons, because it would be trapped by the primers). To avoid this kind of situation, Dr Odriozola also drew up a methodology in his thesis for seeking mutations at these locations, using DHPLC technology. Moreover, he developed a generic methodology, useful for looking for any kind of mutation. Thus, what is involved is a tool that can not only be applied to Forensic Genetics, but to other genetic disciplines also (for example, to hereditary disorders).

Given STR a
'/>"/>

Contact: Amaia Portugal
a.portugal@elhuyar.com
34-943-363-040
Elhuyar Fundazioa
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Repair in the developing heart
2. Space researchers developing tool to help disoriented pilots
3. Carnegie Mellon developing automated systems to enable precision farming of apples, oranges
4. Scientists developing food allergy treatment
5. Pigs and dogs can bridge gap between mice and humans in developing new therapies
6. Nutrigenomics -- developing personalized diets for disease prevention
7. Researchers examine developing hearts in chickens to find solutions for human heart abnormalities
8. Preventing rangeland erosion: Developing better management practices in Iran
9. Veterinarians developing model to help producers, vets make cattle more comfortable
10. Iowa State researchers developing clean, renewable energy for ethanol industry
11. Nutrigenomics -- developing personalized diets for disease prevention -- part 2 just published in OMICS
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:12/24/2014)... launch in December 2014, the 1U™ app has ... to remember their usernames and passwords through replacing the antiquated ... assist people who have struggled to remember usernames and passwords, ... focuses on redefining identity, announced today that it is offering ...
(Date:12/19/2014)... Markets ( http://www.researchandmarkets.com/research/86ncd6/micro_market ) has announced the addition ... Systems Market" report to their offering. ... security market is estimated to grow at a CAGR of ... a larger share in this market, Canada ...
(Date:12/17/2014)... , Dec. 15, 2014  HITLAB SM announced ... audit to confirm its adherence to current U.S. Food ... to conduct regulated smart device and smart phone application ... research quality. "HITLAB is determined to improve ...
Breaking Biology News(10 mins):1U Offers Best Solution to the Username / Password Dilemma: For FREE! 21U Offers Best Solution to the Username / Password Dilemma: For FREE! 3Micro Market Monitor: North America Perimeter Security Systems Market (Intrusion Detection Sensor, Video Surveillance, Communication/Alarm and Notification, Access Control System) 2Micro Market Monitor: North America Perimeter Security Systems Market (Intrusion Detection Sensor, Video Surveillance, Communication/Alarm and Notification, Access Control System) 3HITLAB Completes GCP Audit for FDA Smart Device & App Compliance 2
... with storing nuclear waste and the possibility of it ... no longer be obstacles on the road to cleaner ... University of Notre Dame, led by Thomas E. Albrecht-Schmitt, ... professor of chemistry and biochemistry, showcases Notre Dame Thorium ...
... problems in the world today can be traced back to the ... requiring energy, space and resources to survive, the stress on the ... is enormous. Richard Cardullo , a professor of ... free public lecture on campus in which he will discuss whether ...
... Childrens Research Institute and the University of Melbourne have ... allergy. It is hoped the test would be ... minimise over-diagnosis of peanut allergy in the community. ... standard for diagnosing peanut allergy, and while an oral ...
Cached Biology News:New paper by Notre Dame researchers describes method for cleaning up nuclear waste 2Is Earth overpopulated? 2Test to improve peanut allergy diagnosis 2
(Date:1/22/2015)... today announces the firm brokered a long-term lease totaling 202,492 ... company, at Two Ledgemont Center in Lexington, Massachusetts ... and Brian Cohen , senior vice president, represented Shire ... Hayden Ave. Photo - http://photos.prnewswire.com/prnh/20150122/170765 ...
(Date:1/22/2015)... 2015 Pipette.com has added the ... comprehensive portfolio of Eppendorf products. , The Eppendorf Centrifuge ... Centrifuge 5424/5424 R and receive the following:, ... plus or Eppendorf Reference 2 ,     3 ...
(Date:1/22/2015)... Denville, NJ (PRWEB) January 22, 2015 ... shearing technologies such as the Bioruptor® and complete ... semi-automation system for chromatin immunoprecipitation, alleviating the need ... reagents and controls needed for ChIP of histones ...
(Date:1/22/2015)... , Jan. 22, 2015  Derma Sciences, Inc. ... company focused on advanced wound care, announces that ... tissues, have been added to the Premier, Inc. ... the opportunity for the AMNIOEXCEL® and AMNIOMATRIX® product ...
Breaking Biology Technology:Transwestern?RBJ Advises Shire in 202,000 SF Lease, Creating Boston's Largest Suburban Biotech Campus 2Eppendorf Announces Promotional Bundle Which Includes: Eppendorf Centrifuge 5424, 3-Pack of Pipettes, and Tips - Available Now at Pipette.com 2Diagenode Launches Unique Semi-Automated Solution for Chromatin Immunoprecipitation 2Derma Sciences Expands Access of its AMNIOTIC TISSUE Product Line with New Premier, Inc. Agreement 2Derma Sciences Expands Access of its AMNIOTIC TISSUE Product Line with New Premier, Inc. Agreement 3Derma Sciences Expands Access of its AMNIOTIC TISSUE Product Line with New Premier, Inc. Agreement 4Derma Sciences Expands Access of its AMNIOTIC TISSUE Product Line with New Premier, Inc. Agreement 5
... 28 Boston,Scientific Corporation (NYSE: BSX ) ... that Boston Scientific has completed its acquisition of ... April 16.,Under the terms of the transaction, all ... per share in cash, for a total of,approximately ...
... N.J., May 28 National Stem Cell,Holding, Inc. (OTC: ... steps to,change its name to Proteonomix, Inc. The change ... Michael Cohen, President/CEO, stated: "Our new name reflects the ... development stage and,commencing the commercialization of our technologies. These ...
... Provides Additional Global Corporate Finance, Management and ... Manufacturing ... (TSX: NVN) announced today the appointment of Gordon H.,Busenbark to ... chief financial officer of Xytis Pharmaceuticals, a privately,held development-stage specialty ...
Cached Biology Technology:Boston Scientific Completes Acquisition of CryoCor 2Boston Scientific Completes Acquisition of CryoCor 3National Stem Cell Holding, Inc. Announces the Change of Its Name to Proteonomix, Inc. 2National Stem Cell Holding, Inc. Announces the Change of Its Name to Proteonomix, Inc. 3Nventa Appoints Gordon H. Busenbark to Board of Directors 2
...
GLP expression profiling service on the Affymetrix platform. Starting material can be blood, tissue or RNA. 5 day turnaround. Standard and custom post analyses of microarray data available....
... enables early selection of the best ... quantitative data for sensitivity, dynamic range, ... Furthermore quantitative antibody fingerprints can be ... your antibodies., The full-service package includes ...
... allows for rapid, convenient, and efficient Denatured ... process utilizes a spin column to make ... concentration of proteins which were electro-eluted in ... ion exchangers, offering high binding capacity fo ...
Biology Products: