Navigation Links
Detecting pathogens in waterways: An improved approach
Date:2/8/2011

This press release is available in Spanish.

U.S. Department of Agriculture (USDA) scientists have come up with a way to detect pathogenic Escherichia coli and Salmonella bacteria in waterways at lower levels than any previous method. Similar methods have been developed to detect pathogenic E. coli in meat products, but the approach by the scientists with USDA's Agricultural Research Service (ARS) represents a first for waterways.

ARS is USDA's principal intramural scientific research agency, and this research supports the USDA priority of ensuring food safety.

When health officials test a public beach or lake for Salmonella or E. coli 0157:H7, they use two types of non-pathogenic bacteria, Enterococci and generic E. coli, as indicators. But while the indicators are often detected in contaminated waterways, their abundance doesn't guarantee the presence of either pathogen, according to Michael Jenkins, a microbiologist at the ARS J. Phil Campbell Sr. Natural Resource Conservation Center in Watkinsville, Ga.

These indicator organisms are often reliable, but investigators have detected the indicators in pathogen-free waters and have failed to find them in waters that contained sufficient levels of the pathogens to make someone sick.

The indicators are used as signals because both pathogens are hard to detect directly at levels that will make someone ill: just 100 cells of Salmonella and just 10 to 100 cells of E. coli 0157:H7, the toxic strain of the bacterium. Organic matter in a water sample will throw off current PCR (polymerase chain reaction) technology when it is used as a tool for detection. Salmonella and E. coli outbreaks are often attributed to agricultural operations, so improving ways to track down sources of outbreaks is a major priority.

Jenkins and his ARS colleagues Dinku Endale and Dwight Fisher at Watkinsville combined techniques previously developed to assess water quality and detect pathogens in laboratory settings: a water filtration technique to concentrate the pathogens; a special medium for growing and measuring the number of pathogenic cells; a biochemical testing process; and PCR technology.

They collected water samples from a pond at the Watkinsville site, ran them through a special filter, removed the filter contents and used a centrifuge to spin the filtered contents into a pellet form. They used the suspended pellets to develop cell cultures, confirmed their identity with a genetic method, and determined the concentration found in the original samples.

Their results, published in the Journal of Applied Microbiology, showed the process can be used to detect just a few cells of pathogenic E. coli and Salmonella in a 10-liter water sample, lower levels than any previously detected. Because the system involves collecting cell cultures, it also may lead to developing culture collections that-like a fingerprint database-could be used to identify bacterial strains that are potential sources of future outbreaks.


'/>"/>

Contact: Dennis O'Brien
dennis.obrien@ars.usda.gov
301-504-1624
United States Department of Agriculture - Research, Education and Economics
Source:Eurekalert

Related biology news :

1. Atomic-resolution views suggest function of enzyme that regulates light-detecting signals in eye
2. Men are better at detecting infidelities
3. Detecting tiny twists with a nanomachine
4. Pitt researchers use fluorescence to develop method for detecting mercury in fish
5. Detecting disease in greenhouse plants
6. Novel veterinary procedure detecting life-threatening injuries touted
7. New method for detecting explosives
8. $1.6 million grant to lead development of resistance-detecting field kit
9. Researchers develop new, more-sensitive assay for detecting DNA methylation in colon cancer
10. Simple test could offer cheap solution to detecting landmines
11. Toward a urine test for detecting colon cancer
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/11/2017)... April 11, 2017 NXT-ID, Inc. (NASDAQ: ... company, announces the appointment of independent Directors Mr. Robin ... its Board of Directors, furthering the company,s corporate governance and ... Gino Pereira ... look forward to their guidance and benefiting from their considerable ...
(Date:4/5/2017)... -- The Allen Institute for Cell Science today announces the ... and dynamic digital window into the human cell. The ... of deep learning to create predictive models of cell ... growing suite of powerful tools. The Allen Cell Explorer ... available resources created and shared by the Allen Institute ...
(Date:4/5/2017)... -- KEY FINDINGS The global market for ... of 25.76% during the forecast period of 2017-2025. The ... the growth of the stem cell market. ... INSIGHTS The global stem cell market is segmented on ... stem cell market of the product is segmented into ...
Breaking Biology News(10 mins):
(Date:10/12/2017)... , ... October 12, 2017 , ... ... Vilnius, Lithuania, announced today that they have entered into a multiyear collaboration to ... provide CRISPR researchers with additional tools for gene editing across all applications. , ...
(Date:10/12/2017)... (PRWEB) , ... October 12, 2017 , ... ... Surgical Wound Market with the addition of its newest module, US Hemostats & ... market for thrombin hemostats, absorbable hemostats, fibrin sealants, synthetic sealants and biologic sealants ...
(Date:10/12/2017)... (PRWEB) , ... October 12, ... ... ) has launched Rosalindâ„¢, the first-ever genomics analysis platform specifically designed for ... complexity. Named in honor of pioneering researcher Rosalind Franklin, who made a ...
(Date:10/11/2017)... ... October 11, 2017 , ... The CRISPR-Cas9 ... enabling overexpression experiments and avoiding the use of exogenous expression plasmids. The simplicity ... for performing systematic gain-of-function studies. , This complement to loss-of-function studies, such ...
Breaking Biology Technology: