Navigation Links
Despite damage, membrane protein structure can be seen using new X-ray technology, study reveals
Date:12/20/2010

Australian researchers have identified a way to measure the structure of membrane proteins despite being damaged when using X-ray Free-Electron Lasers (XFELs), a discovery that will help fast track the development of targeted drugs using emerging XFELs technology.

About 70% of drugs on the market today depend on the activity of membrane proteins, which are complex molecules that form the membranes of the cells in our body.

A major problem for the design of new pharmaceuticals, often known as the "membrane protein problem", is that they do not form the crystals needed to enable further investigation of the structure to design better drugs.

A major international effort is being mounted to determine the structures of membrane proteins using XFELs - large facilities that create such a bright beam of X-rays it is possible to see the X-ray light bouncing off a single molecule without forming a crystal.

Professor Keith Nugent, Laureate Professor and ARC Federation Fellow and Director of the Australian Research Council Centre of Excellence for Coherent X-ray Science (CXS) at the University of Melbourne said a key problem was that the light from an XFEL was so bright a molecule would start to disintegrate in less than one thousandth of a millionth of a millionth of a second.

In a paper published today in the journal Nature Physics, Professor Nugent and Associate Professor Harry Quiney from the ARC Centre of Excellence for Coherent X-ray Science (CXS) have developed a method by which the damage from the XFEL pulse may be included in the data analysis.

Associate Professor Quiney, also of the School of Physics at the University of Melbourne, said results showed that high-resolution molecular structures may be obtained from X-ray scattering data using a few-femtosecond pulse from an XFEL, even if the interaction resulted in significant electronic damage to the target.

"This result has far-reaching implications for the future development of structural biology, because it removes a significant obstacle to the practical realisation of the molecular microscope using XFEL sources," he said.

It also provides important insights into the complex, turbulent and poorly-understood interactions that are driven by the interaction of an XFEL pulse with an atom, molecule or solid.

Their approach uses sophisticated molecular physics and careful data analysis to determine a new approach to measuring molecular structure.

Although still at the theoretical and computation level when put into practice this discovery will remove a major road-block in the path to solving the membrane protein problem.

This year, CXS signed an agreement with Japanese colleagues and will host the 4th Asia-Oceania Workshop on Science with X-ray Free Electron Lasers in 2011.

Professor Nugent said this was an extremely exciting time for X-ray science.

"My colleagues and I are convinced that our recent work is a critically important step forward," he said.

"We are very much looking forward to working with our Japanese colleagues in the coming years".

The first XFEL started operating at Stanford in California in 2009 and the second, the SPring8 facility in Japan, will start in 2011. A third is under construction in Europe to commence in 2014.


'/>"/>

Contact: Diane Squires
dsquires@unimelb.edu.au
043-275-4232
University of Melbourne
Source:Eurekalert

Related biology news :

1. Forest pests accumulating despite regulations
2. Racial differences in breast cancer treatment persist despite similar economics
3. Despite countless changes, original HIV infection lurks within
4. Pesky aphid thrives despite weak immune system
5. Despite risk, older African-Americans more likely than others to avoid flu vaccine
6. Despite claims, UK did not gas Iraqis in the 1920s, new research finds
7. Birds in Flint Hills of Kansas, Oklahoma face population decline despite large habitat
8. Organic soils continue to acidify despite reduction in acidic deposition
9. Despite peacenik reputation, bonobos hunt and eat other primates too
10. When it comes to brain damage, blankets take the place of drugs
11. UT Southwestern researchers probe kidney damage, protection in lupus
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/20/2016)... 20, 2016 Securus Technologies, a leading ... for public safety, investigation, corrections and monitoring announced ... it has secured the final acceptance by all ... Managed Access Systems (MAS) installed. Furthermore, Securus will ... be installed by October, 2016. MAS distinguishes between ...
(Date:6/9/2016)... , June 9, 2016 ... Police deploy Teleste,s video security solution to ensure the safety ... France during the major tournament Teleste, ... communications systems and services, announced today that its video security ... to back up public safety across the country. ...
(Date:6/2/2016)... --  The Weather Company , an IBM Business (NYSE: ... in which consumers will be able to interact with IBM ... voice or text and receive relevant information about the product ... have long sought an advertising solution that can create a ... and valuable; and can scale across millions of interactions and ...
Breaking Biology News(10 mins):
(Date:6/27/2016)... Hill, N.C. (PRWEB) , ... June 27, 2016 ... ... U.S. commercial operations for Amgen, will join the faculty of the University ... serve as adjunct professor of strategy and entrepreneurship at UNC Kenan-Flagler, with a ...
(Date:6/24/2016)... Epic Sciences unveiled a liquid biopsy ... PARP inhibitors by targeting homologous recombination deficiency (HRD) ... test has already been incorporated into numerous clinical ... Over 230 clinical trials are investigating ... PARP, ATM, ATR, DNA-PK and WEE-1. Drugs targeting ...
(Date:6/23/2016)... ... June 23, 2016 , ... UAS LifeSciences, one of ... their brand, UP4™ Probiotics, into Target stores nationwide. The company, which has been ... Target to its list of well-respected retailers. This list includes such fine stores ...
(Date:6/23/2016)... Houston Methodist Willowbrook Hospital has signed ... to serve as their official health care provider. ... will provide sponsorship support, athletic training services, and ... volunteers, athletes and families. "We are ... and to bring Houston Methodist quality services and ...
Breaking Biology Technology: