Navigation Links
Delving into the molecular mechanism behind deep-sea bacteria's pressure tolerance
Date:7/26/2012

The Mariana Trench is the deepest feature of the Earth's surface. The water column there exerts a pressure of more than one thousand times normal atmospheric pressure at sea level, enough pressure to crush an SUV. Yet many organisms thrive in this seemingly inhospitable environment. A Japanese research team has been investigating how deep-sea bacteria adapt to such high-pressure conditions. They have identified a structural change that confers pressure-resistant properties on a particular protein found in bacteria. The findings, which the team will present at the meeting of the American Crystallographic Association (ACA), held July 28-Aug. 1, in Boston, Mass., may one day help guide the design of enzymes for use in high-pressure chemical industrial processes.

In general, pressure, like that caused by a water column thousands of feet deep, deforms proteins. As the proteins change shape, water can penetrate the protein's interior. Some proteins are better able to resist this incursion of water, but the molecular mechanisms of the pressure resistance aren't yet well understood.

"Our group is focusing on high-pressure protein crystallography, using 3-isopropylmalate dehydrogenase (IPMDH) as a model protein. The goal is to delve into the principles of the molecular mechanism of the pressure tolerance of proteins by comparing the structures of IPMDHs from organisms that thrive in high-pressure environments and those that are sensitive to high-pressure pressure environments," explains Nobuhisa Watanabe, a professor at the Synchrotron Radiation Research Center, Nagoya University.

To create the high pressures necessary for their studies, the team uses a diamond anvil cell (DAC), which consists of two opposing diamonds with a gasket compressed between the culets (the small, flat facet at the bottom of the diamonds).

The team's big discovery so far is that the initial water penetration at the molecular surface of the side opposite to the active site of IPMDH is unique.

"At the site of the penetration, there is a difference of amino acid between IPMDHs from bacteria that thrive in high-pressure environments and those that are sensitive to it. Based on this data, we substituted one amino acid at the site of the IPMDH from pressure-sensitive bacteria and checked its activity under pressure," says Watanabe. "And as we expected, only this one residue-substituted IPMDH, which has 364 amino acids in total, achieved pressure resistance comparable to the bacteria that thrive in high-pressure environments."

This means that it may soon be possible to synthesize designer pressure-resistant proteins. The team plans to continue their high-pressure studies of several other proteins to try to discover the physical principles behind pressure resistance mechanisms that enable bacteria to thrive in high-pressure conditions.


'/>"/>
Contact: Catherine Meyers
cmeyers@aip.org
301-209-3088
American Institute of Physics
Source:Eurekalert

Related biology news :

1. Speed and power of X-ray laser helps unlock molecular mysteries
2. Highlighting molecular clues to the link between childhood maltreatment and later suicide
3. MARC travel award announced for the 2012 GSA Yeast Genetics and Molecular Biology Meeting
4. How bacteria change movement direction in response to oxygen: Molecular interactions unravelled
5. The activity of a bacterial effector protein seen in molecular detail
6. Hitting snooze on the molecular clock: Rabies evolves slower in hibernating bats
7. Molecular spectroscopy tracks living mammalian cells in real time as they differentiate
8. PNAS: Precise molecular surgery in the plant genome
9. 5th Annual Advances in Biomolecular Engineering Symposium
10. 2012 Forecast for US Molecular Diagnostics Market Now Available From Global Information Inc.
11. Beyond the microscope: Identifying specific cancers using molecular analysis
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:1/21/2016)... January 21, 2016 ... new market research report "Emotion Detection and Recognition Market by ... Tools (Facial Expression, Voice Recognition and Others), Services, ... forecast to 2020", published by MarketsandMarkets, the global ... reach USD 22.65 Billion by 2020, at a ...
(Date:1/20/2016)... , Jan. 20, 2016  Synaptics Incorporated (NASDAQ: ... interface solutions, today announced sampling of S1423, its ... wearables and small screen applications including smartwatches, fitness ... Supporting round and rectangular shapes, as well as ... performance with moisture on screen, while wearing gloves, ...
(Date:1/13/2016)... --> --> ... report titled - Biometric Sensors Market - Global Industry Analysis, ... to the report, the global biometric sensors market was valued at ... US$1,625.8 mn by 2023, expanding at a CAGR of ... the biometric sensors market is expected to reach 1,799.6 ...
Breaking Biology News(10 mins):
(Date:2/11/2016)... , February 11, 2016 ... Corporation ("PositiveID" or "Company") (OTCQB: PSID), a life ... today that its Thermomedics subsidiary, which markets the ... its growth plan in January 2016, including entering ... increasing sequential monthly sales growth, and establishing several ...
(Date:2/11/2016)... , ... February 11, 2016 , ... ... more than 150 years, continues today to pursue the highest level of accuracy ... analytical instruments: the AR9 Refractometer and the AR5 Refractometer. Accurate, reliable and ...
(Date:2/11/2016)... ... 11, 2016 , ... Global Stem Cells Group, ... Ecuador. The new facility will provide advanced protocols and state-of-the-art techniques in cellular ... , The new GSCG clinic is headed by four prominent Ecuadorian physicians, ...
(Date:2/10/2016)... , February 10, 2016 Early-career researchers ... , Peru , Uganda and ... life-enhancing work in health and nutrition   Indonesia ... Uganda and Yemen are being ... and epidemiology. They are also celebrated for mentoring young women scientists who ...
Breaking Biology Technology: