Navigation Links
Delving into the molecular mechanism behind deep-sea bacteria's pressure tolerance
Date:7/26/2012

The Mariana Trench is the deepest feature of the Earth's surface. The water column there exerts a pressure of more than one thousand times normal atmospheric pressure at sea level, enough pressure to crush an SUV. Yet many organisms thrive in this seemingly inhospitable environment. A Japanese research team has been investigating how deep-sea bacteria adapt to such high-pressure conditions. They have identified a structural change that confers pressure-resistant properties on a particular protein found in bacteria. The findings, which the team will present at the meeting of the American Crystallographic Association (ACA), held July 28-Aug. 1, in Boston, Mass., may one day help guide the design of enzymes for use in high-pressure chemical industrial processes.

In general, pressure, like that caused by a water column thousands of feet deep, deforms proteins. As the proteins change shape, water can penetrate the protein's interior. Some proteins are better able to resist this incursion of water, but the molecular mechanisms of the pressure resistance aren't yet well understood.

"Our group is focusing on high-pressure protein crystallography, using 3-isopropylmalate dehydrogenase (IPMDH) as a model protein. The goal is to delve into the principles of the molecular mechanism of the pressure tolerance of proteins by comparing the structures of IPMDHs from organisms that thrive in high-pressure environments and those that are sensitive to high-pressure pressure environments," explains Nobuhisa Watanabe, a professor at the Synchrotron Radiation Research Center, Nagoya University.

To create the high pressures necessary for their studies, the team uses a diamond anvil cell (DAC), which consists of two opposing diamonds with a gasket compressed between the culets (the small, flat facet at the bottom of the diamonds).

The team's big discovery so far is that the initial water penetration at the molecular surface of the side opposite to the active site of IPMDH is unique.

"At the site of the penetration, there is a difference of amino acid between IPMDHs from bacteria that thrive in high-pressure environments and those that are sensitive to it. Based on this data, we substituted one amino acid at the site of the IPMDH from pressure-sensitive bacteria and checked its activity under pressure," says Watanabe. "And as we expected, only this one residue-substituted IPMDH, which has 364 amino acids in total, achieved pressure resistance comparable to the bacteria that thrive in high-pressure environments."

This means that it may soon be possible to synthesize designer pressure-resistant proteins. The team plans to continue their high-pressure studies of several other proteins to try to discover the physical principles behind pressure resistance mechanisms that enable bacteria to thrive in high-pressure conditions.


'/>"/>
Contact: Catherine Meyers
cmeyers@aip.org
301-209-3088
American Institute of Physics
Source:Eurekalert

Related biology news :

1. Speed and power of X-ray laser helps unlock molecular mysteries
2. Highlighting molecular clues to the link between childhood maltreatment and later suicide
3. MARC travel award announced for the 2012 GSA Yeast Genetics and Molecular Biology Meeting
4. How bacteria change movement direction in response to oxygen: Molecular interactions unravelled
5. The activity of a bacterial effector protein seen in molecular detail
6. Hitting snooze on the molecular clock: Rabies evolves slower in hibernating bats
7. Molecular spectroscopy tracks living mammalian cells in real time as they differentiate
8. PNAS: Precise molecular surgery in the plant genome
9. 5th Annual Advances in Biomolecular Engineering Symposium
10. 2012 Forecast for US Molecular Diagnostics Market Now Available From Global Information Inc.
11. Beyond the microscope: Identifying specific cancers using molecular analysis
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/29/2017)... CHICAGO , March 29, 2017  higi, the ... ecosystem in North America , today ... Partners and the acquisition of EveryMove. The new investment ... extensive set of tools to transform population health activities ... and lifestyle data. higi collects and secures ...
(Date:3/27/2017)... N.Y. , March 27, 2017  Catholic ... Information and Management Systems Society (HIMSS) Analytics for ... EMR Adoption Model sm . In addition, CHS ... of U.S. hospitals using an electronic medical record ... for its high level of EMR usage in ...
(Date:3/24/2017)... Research and Markets has announced the addition ... Trends - Industry Forecast to 2025" report to their offering. ... The Global Biometric ... of around 15.1% over the next decade to reach approximately $1,580 ... market estimates and forecasts for all the given segments on global ...
Breaking Biology News(10 mins):
(Date:10/11/2017)... ... October 11, 2017 , ... ComplianceOnline’s Medical Device Summit is back for its ... 2018 in San Francisco, CA. The Summit brings together current and former FDA office ... directors and government officials from around the world to address key issues in device ...
(Date:10/11/2017)... ... 11, 2017 , ... Disappearing forests and increased emissions are the main causes ... each year. Especially those living in larger cities are affected by air pollution related ... the most pollution-affected countries globally - decided to take action. , “I knew I ...
(Date:10/10/2017)... ... October 10, 2017 , ... ... company advancing targeted antibody-drug conjugate (ADC) therapeutics, today confirmed licensing rights that ... Polymerized Liposomal Nanoparticle), a technology developed in collaboration with Children’s Hospital Los ...
(Date:10/10/2017)... 10, 2017 SomaGenics announced the receipt of ... develop RealSeq®-SC (Single Cell), expected to be the first ... (including microRNAs) from single cells using NGS methods. The ... to accelerate development of approaches to analyze the heterogeneity ... "New techniques for measuring levels of mRNAs in individual ...
Breaking Biology Technology: