Navigation Links
Deep sequencing study reveals new insights into human transcriptome
Date:7/8/2008

In a collaborative project scientists from the Max-Planck-Institute for Molecular Genetics in Berlin (MPI MolGen), Germany and Genomatix with a business in Munich, Germany and Ann Arbor, MI, USA, applied next generation sequencing and analysis methods to generate an unprecedented view at the human transcriptome.

Deep sequencing of transcripts from two human cell lines revealed so far unrecognized complexity and variability of the human transcriptome. They found that 34% of the polyadenylated transcriptome mapped to so far non-annotated genomic regions. Obviously a large number of novel gene candidates are active in the cell lines under study.

In addition, a global survey of mRNA splicing events identified 94,241 splice junctions, of which 4,096 are novel, and showed that exon skipping is the most prevalent form of alternative splicing.

Details are presented in the Science report of Sultan et al. "A Global View of Gene Activity and Alternative Splicing by Deep Sequencing of the Human Transcriptome", published online at Science Express (www.scienceexpress.org). Annotation and data visualization is publicly available at http://www.genomatix.de/MPI.html .

Dr. Marie-Laure Yaspo, Group Leader at the MPI MolGen and head scientist of the study states: " Deep sequencing allows for the first time to explore directly the complexity and dynamics of the human transcriptome with a reasonable effort. This will lead to a new picture of the mammalian genome annotation far beyond the current state of the art. We provide here global features of alternative splicing events in human cell lines. Such a comparison of within-cell and between-cell alternative splicing events, combined with the simultaneous analysis of gene expression has never been presented before. It becomes clear that the so far available methods only delivered a part of the transcriptional landscape of mammalian cells, especially if gene regulation analysis is considered"

Dr. Martin Seifert, Vice President Business Development and Consulting at Genomatix says:

"The main biological impact is the observation of a new dimension in complexity and variability. Based on the method we could find a significant number of new transcriptional units and splice variants. Our analyses clearly show that transcription is a highly dynamic and variable process. We learned a lot by having access to such high quality data and co-developed necessary new analysis strategies with the MPI MolGen. Especially users of our brand new Genomatix Genome Analyzer will benefit from our experiences along the project, since they have access to all developed strategies."


'/>"/>

Contact: Martin Seifert
seifert@genomatix.de
498-959-9766
Genomatix Software GmbH
Source:Eurekalert

Related biology news :

1. 454 Sequencing uncovers a genetic basis for different social behaviors in wasp
2. 454 Sequencing: Science paper describes a novel, highly efficient method of sequencing ancient DNA
3. UC Irvine receives $2.18M to explore nano advancements in DNA sequencing
4. Microarray sequence capture speeds large-scale resequencing of targeted genomic regions
5. New technique could dramatically lower costs of DNA sequencing
6. DOE JGI Community Sequencing Program delivers first moss genome
7. K-State contributions to red flour beetle genome sequencing featured in March 27 issue of Nature
8. Beijing Genomics Institute adds AB SOLiD system to its next generation sequencing technologies
9. Genetic sequencing of protein from T. rex bone confirms dinosaurs link to birds
10. Argonne-University of Chicago joint venture bolsters genomic sequencing capabilities
11. US Department of Energy Joint Genome Institute announces new genome sequencing projects
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/18/2017)... , April 18, 2017  Socionext Inc., a global expert ... a media edge server, the M820, which features the company,s hybrid ... software provided by Tera Probe, Inc., will be showcased during the ... the NAB show at the Las Vegas ... ...
(Date:4/13/2017)... April 13, 2017 According to a new market ... Identity Analytics, Identity Administration, and Authorization), Service, Authentication Type, Deployment Mode, Vertical, ... Market is expected to grow from USD 14.30 Billion in 2017 to ... of 17.3%. ... MarketsandMarkets Logo ...
(Date:4/11/2017)... , April 11, 2017 No two ... researchers at the New York University Tandon School ... Engineering have found that partial similarities between prints ... used in mobile phones and other electronic devices ... The vulnerability lies in the fact that ...
Breaking Biology News(10 mins):
(Date:5/23/2017)... ... ... Bacterial biofilms, surface adherent communities of bacteria that are encased in a ... and catheter infections to gum disease and the rejection of medical implants. Though ... year, there is currently a paucity of means for preventing their formation or treating ...
(Date:5/22/2017)... ... ... Stratevi, a boutique firm that partners with healthcare companies to creatively develop and ... downtown Boston at 745 Atlantic Ave. , “We are seeing that even the ... value they provide, not just to patients, but also payers. Having a presence in ...
(Date:5/21/2017)... ... May 19, 2017 , ... Ovation Fertility scientists’ ... American Association of Bioanalysts (AAB) and the College of Reproductive Biology (CRB) today ... AAB’s commitment to excellence in clinical laboratory services and regulations. , “We ...
(Date:5/19/2017)... Philadelphia, PA (PRWEB) , ... May 19, 2017 ... ... for its QED Proof-of-Concept Program. Academic researchers with technologies ripe for commercialization, ... Pennsylvania, New Jersey and Delaware, are encouraged to submit proposals. QED, now in ...
Breaking Biology Technology: