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Dead or alive? A new test to determine viability of soybean rust spores
Date:12/11/2012

ngicide-resistant strains.

"In 2005, there were some farms in the Midwest and the north-central area that were sprayed with fungicide because the word was out that somebody found some spores," Hartman said.

Hartman, Ramya Vittal, a postdoctoral researcher in the Laboratory for Soybean Disease Research, and James Haudenshield, a USDA-ARS research plant pathologist, have just developed a method that uses two different staining techniques to determine spore viability.

The first technique uses two dyes: carboxyfluorescein diacetate (CFDA) and propidium iodide (PI). Viable spores stain green with CFDA; non-viable spores counterstain red with PI.

The second technique uses a two-color fluorescent viability probe that causes cylindrical vacuolar structures to form within living spores, which then fluoresce red. Non-viable spores show only faint fluorescence.

Hartman said that these tests are rapid and reliable. Early detection coupled with timely fungicide application can help slow the pathogen's spread and minimize yield losses.

The next step is to integrate this method with passive spore sampling to develop a tool to detect and monitor the movement of viable P. pachyrhizi spores during the soybean growing season.


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Contact: Susan Jongeneel
sjongene@illinois.edu
217-333-3291
University of Illinois College of Agricultural, Consumer and Environmental Sciences
Source:Eurekalert

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