Navigation Links
DNA repair mechanisms relocate in response to stress

Like doctors making house calls, some DNA repair enzymes can relocate to the part of the cell that needs their help, a collaborative team of scientists at Emory University School of Medicine has found.

The signal that prompts relocation is oxidative stress, an imbalance of cellular metabolism connected with several human diseases.

The study integrated the expertise of three Emory groups and resulted in a new level of understanding of the cell's response to genetic damage. The finding could lead to new targets for anti-cancer drugs that interfere with DNA repair, says Paul Doetsch, PhD, professor of biochemistry, radiation oncology, and hematology and oncology at Emory University School of Medicine.

The results were published in the February 1 issue of Molecular and Cellular Biology. The journal's editors chose an image of yeast cells with fluorescent DNA repair enzymes for the cover.

"DNA damage and oxidative stress are very closely related," Doetsch says. "For example, the way radiation inflicts most of its damage on DNA is through oxidative stress. The more we know about how cells respond to oxidative stress, the more chances there could be to influence those responses for diagnostic or therapeutic purposes."

The DNA inside cells is continually under assault by heat, radiation and oxygen. Cells have an extensive set of repair enzymes that comb through DNA, continually excising and re-copying damaged segments. To complicate matters, mitochondria (cells' miniature power plants) have their own DNA.

Working with Doetsch, Emory graduate students Lyra Griffiths and Dan Swartzlander, and biochemists Anita Corbett and Keith Wilkinson, genetically modified strains of yeast so that two different DNA repair enzymes would be fluorescent. They were able to follow the enzymes around the cell when yeast was exposed to hydrogen peroxide, causing oxidative stress, or to other chemicals causing DNA damage.

One DNA repair enzyme they studied, Ntg1, moves to the nucleus or the mitochondria depending on where DNA damage is concentrated, the authors found. In contrast, a related enzyme, Ntg2, stays in the nucleus under all conditions.

Cells appear to direct Ntg1's relocation by briefly attaching a small protein called SUMO to what needs to be moved around, the authors found. SUMO is found in fungi, plants and animals and is already being investigated by several research groups as a possible target for anti-cancer drugs.


Contact: Holly Korschun
Emory University

Related biology news :

1. Human derived stem cells can repair rat hearts damaged by heart attack
2. Pittsburgh scientists identify human source of stem cells with potential to repair muscle
3. Enzyme alerts cells powerful army to repair DNA damage
4. Seattle Childrens Hospital leads $23.7 million NIH grant to study gene repair
5. Simulation reveals how body repairs balance after damage
6. Using nanotubes to detect and repair cracks in aircraft wings, other structures
7. Enhanced DNA-repair mechanism can cause breast cancer
8. Yale receives $8.4 million to study DNA repair in cancer cells
9. Building the future -- 21st century nano tools to repair the nervous system
10. Researchers probe a DNA repair enzyme
11. Wildcat Power Cord repairs cruciate ligament in dairy cows knee
Post Your Comments:
(Date:11/10/2015)... LONDON , Nov. 10, 2015 /PRNewswire/ ... segmented on the basis of product, type, ... segments included in this report are consumables, ... this report are safety biomarkers, efficacy biomarkers, ... in this report are diagnostics development, drug ...
(Date:11/2/2015)...  SRI International has been awarded a contract of ... to the National Cancer Institute (NCI) PREVENT Cancer Program ... modern testing and support facilities, and analytical instrumentation to ... studies to evaluate potential cancer prevention drugs. ... Drug Development Program is an NCI-supported pipeline to bring ...
(Date:10/29/2015)... health pioneer, Joseph C. Kvedar , MD, describes ... wellness, and the business opportunities that arise from it ... Healthy Things . Long before health and wellness ... vice president, Connected Health, Partners HealthCare, was creating a ... the hospital or doctor,s office into the day-to-day lives ...
Breaking Biology News(10 mins):
(Date:12/1/2015)... Today the Allen Institute announced the opening ... South Lake Union neighborhood, the city,s biotechnology hub. ... Westlake Avenue North, the 270,000 square foot life sciences ... Science and the Allen Institute for Cell Science. ... the Allen Institute. "We started by building a map ...
(Date:12/1/2015)... MOUNTAIN VIEW, Calif. , Dec. 1, 2015 ... introduce the Transformational Health program. This program addresses ... this period of change. ... --> Logo - ... Understanding every aspect of healthcare, as well as ...
(Date:12/1/2015)... 2015 ... "2016 Europe Cell Surface Markers: Country ... Strategies, Opportunities for Suppliers--France, Germany, Italy, Spain, ... --> ) has announced the ... Surface Markers: Country Volume and Sales Forecasts, ...
(Date:12/1/2015)... MD (PRWEB) , ... December 01, 2015 , ... ... solutions provider, announces that its best selling system laboratory animal colony management software ... Cloud today, without investing in on-site IT resources., , ...
Breaking Biology Technology: