Navigation Links
DNA origami nano-tool provides important clue to cancer

Researchers at Karolinska Institutet in Sweden have headed a study that provides new knowledge about the EphA2 receptor, which is significant in several forms of cancer. This is important knowledge in itself but just as important is how this study, which is published in the highly respected journal Nature Methods, was conducted. The researchers used the method of DNA origami, in which a DNA molecule is shaped into a nanostructure, and used these structures to test theories about cell signalling.

It was previously known that the EphA2 receptor played a part in several forms of cancer, such as breast cancer. The ligand, i.e., the protein that communicates with the receptor, is known as an ephrin molecule. Researchers have had a hypothesis that the distance between different ligands in this case the distance between ephrin molecules affects the level of activity in the communicating receptor of the adjacent cells.

The Swedish researchers set out to test this hypothesis. They used DNA building blocks to form a stable rod. This has then been used as a very accurate measure of the distance between molecules.

"We use DNA as the construction material for a tool that we can experiment with", says Bjrn Hgberg, principal investigator at the Department of Neuroscience. "The genetic code of the DNA in these structures is less important in this case."

The researchers attached proteins, or ephrins, to the DNA rod at various intervals, for example 40 or 100 nanometres apart. The DNA rods were then placed in a solution containing breast cancer cells. In the next step, the researchers looked at how active EphA2 was in these cancer cells.

It turned out that if the ephrin molecules were placed close together on the DNA rod, the receptor in question became more active in the cancer cells, and the cells also became less invasive in respect of the surrounding cells, which could be an indication that they became less prone to metastasis. This was true even though the amount of protein was the same throughout the experiments, i.e., the number of attached molecules remained the same.

"For the very first time, we have been able to prove this hypothesis: the activity of EphA2 is influenced by how closely spaced the ligands are on the surrounding cells", says Bjrn Hgberg. "This is an important result in itself, but the point of our study is also that we have developed a method for examining how cells react to nearby cells in a controlled environment, using our custom DNA nano-calipers."

The researchers describe the cell communication as a form of Braille, where the cells somehow sense the protein patterns of nearby cells, and where the important thing is not only the amount of proteins, but to a great extent the distance between them as well. This study found that a cluster of proteins would communicate more actively than sparsely spaced proteins, even if the concentration was the same.

"This is a model that can help us learn more about the importance of the spatial organization of proteins in the cell membrane to how cells communicate with each other, something that will hopefully pave the way for a brand new approach to pharmaceuticals in the long term", says Ana Teixeira, a principal investigator at the Department of Cell and Molecular Biology. "Today, the function of the pharmaceuticals is often to completely block proteins or receptors, but it is possible that we should rather look at the proteins in their biological context, where the clustering and placement of various proteins are relevant factors for the effect of a drug. This is probably an area where there is important knowledge to obtain, and this is a way of doing it."


Contact: Press Office
Karolinska Institutet

Related biology news :

1. Protein origami: Quick folders are the best
2. Origami inspires research into materials that self-assemble when exposed to light
3. The shape of things to come: NIST probes the promise of nanomanufacturing using DNA origami
4. Discovery provides insights on how plants respond to elevated CO2 levels
5. Newly identified gene provides reliable visual cue for oil palm fruit ripeness
6. Research provides new theory on cause of ice age 2.6 million years ago
7. Africas poison apple provides common ground for saving elephants, raising livestock
8. New mobile app provides faster, more accurate measurement of respiratory rate
9. Common bean genome sequence provides powerful tools to improve critical food crop
10. New research provides better understanding of endometriosis
11. Pesticides: Research provides new insights into their effects on shrimps and snails
Post Your Comments:
Related Image:
DNA origami nano-tool provides important clue to cancer
(Date:10/29/2015)... Oct. 29, 2015  The J. Craig Venter Institute ... "DNA Synthesis and Biosecurity: Lessons Learned and Options for ... Health and Human Services guidance for synthetic biology providers ... --> --> Synthetic ... the potential to pose unique biosecurity threats. It now ...
(Date:10/29/2015)... health pioneer, Joseph C. Kvedar , MD, describes ... wellness, and the business opportunities that arise from it ... Healthy Things . Long before health and wellness ... vice president, Connected Health, Partners HealthCare, was creating a ... the hospital or doctor,s office into the day-to-day lives ...
(Date:10/27/2015)... -- In the present market scenario, security is one ... verticals such as banking, healthcare, defense, electronic gadgets, and ... secure & simplified access control and growing rate of ... bank accounts, misuse of users, , and so on. ... and smartphones are expected to provide potential opportunities for ...
Breaking Biology News(10 mins):
(Date:11/25/2015)... , Nov. 25, 2015 /PRNewswire/ - Aeterna Zentaris Inc. ... its business and prospects remain fundamentally strong and ... (zoptarelin doxorubicin) recently received DSMB recommendation to continue ... following review of the final interim efficacy and ... Primary Endpoint in men with heavily pretreated castration- ...
(Date:11/25/2015)... ... November 25, 2015 , ... Jessica Richman and Zachary Apte, ... their initial angel funding process. Now, they are paying it forward to other ... stage investments in the microbiome space. In this, they join other successful ...
(Date:11/24/2015)... SAN DIEGO , Nov. 24, 2015 Halozyme Therapeutics, ... Jaffray Healthcare Conference in New York on ... Dr. Helen Torley , president and CEO, will provide a ... New York at 1:00 p.m. ET/10:00 a.m. ... communication and investor relations, will provide a corporate overview. --> ...
(Date:11/24/2015)... 24, 2015 /PRNewswire/ - Aeterna Zentaris Inc. (NASDAQ:  AEZS) (TSX: ... behalf of the Toronto Stock Exchange, confirms that as ... no corporate developments that would cause the recent movements ... --> About Aeterna Zentaris Inc. ... --> Aeterna Zentaris is a specialty biopharmaceutical company ...
Breaking Biology Technology: