Navigation Links
Cross kingdom conflicts on a beetle's back
Date:10/3/2008

BOSTON, Mass. (Oct. 2, 2008)Researchers from Harvard Medical School and the University of Madison-Wisconsin have discovered how beetles and bacteria form a symbiotic and mutualistic relationshipone that ultimately results in the destruction of pine forests. In addition, they've identified the specific molecule that drives this whole phenomenon.

The context of this discovery can easily be imagined as a story arc that includes some of the most unlikely characters and props.

Setting: The interior of a pine tree.

Enter the protagonist: The pine beetle, boring its way through the bark, a five millimeter arthropod ready to go into labor and lay a few hundred eggs. Tucked in a specialized storage compartment in its shell, the beetle has a ready supply of spores for Entomocorticium, a nourishing fungal baby food for the beetle's gestating larvae.

Enter the antagonist: The mite, a microscopic interloper that secretly hitched a ride on the beetle.

Conflict: Unbeknownst to mother pine beetle, the mite has snuck in a supply of Ophiostoma minus, a pathogenic fungi that can wipe out the entire supply of fungal larvae food. The mite releases this toxin.

Climax: Will the baby beetles die of starvation?

Resolution: Catching the mite off guardas well as the scientists conducting the study!the mother beetle is ready with actinomycetes, a bacteria that neutralizes the toxic fungi by means of a tiny fatty acid.

Conclusion: While actinomycetes rescues the baby beetles from certain starvation, the larvae-friendly Entomocorticium softens up the pine, allowing the fledgling beetles to eat not only the fungi but the tree itself. Soon, the young beetles leave to begin their new lives. Mother beetle gathers up the remaining supply of Entomocorticium and heads for another tree. The beetles live, and the infernal mite is thwarted.

Surprise ending: The camera pans back, and we quickly realize that the beetles' success has cost the tree its life. An aerial view reveals miles and miles of dead pine forest, and, as the ominous audio track implies, scores of pine beetles will continue moving from tree to tree leaving ravished forests in their wake.

"So you have a beetle, a mite, a tree, two kinds of fungi, and a bacterium," says Jon Clardy, Harvard Medical School professor of biological chemistry and molecular pharmacology who, along with Cameron Currie from the University of Madison-Wisconsin, is co-senior author on the study. "Discovering this particular bacterium, and the active molecule, has added the molecular dimension to this chemical ecology of this complex multi-lateral system. It highlights the importance of bacteria in ways that people don't really even think about."

The findings will be published in the October 3 issue of Science.

The ground work for this study began in 1999 when Currie published a paper demonstrating how leafcutter ants mediate their fungal environment through bacteria. Suspecting that this phenomenon may be common throughout the animal kingdom, Currie teamed up with Clardy to examine the pine beetle.

Pine beetles are like little landscape engineers, drilling through the bark and into pine trees, using fungus to create an environment in which to lay their eggs. As a result of this activity, thousands of miles of trees are destroyed each year, often resulting in widespread forest fires. Regions such as western Canada are particularly affected by this.

Experts have known that just like the fungus-growing ants, pine beetles also use fungus to feed their larvae, and that they often managed to avoid the adverse affects of pathogenic fungi often present in the tree. But the precise means by which they interact with fungal microbes has never been demonstrated.

Currie and research assistant Jarrod Scott discovered that the beetle carries a bacteria in a specialized compartment, and after a series of experiments found that the bacteria produced an antifungal agent that killed the pathogenic fungi snuck in by the trespassing mite.

In order to delve deeper into how the bacteria works, Dong-Chan Oh, a postdoctoral researcher in Clardy's Harvard Medical School lab, used a variety of laboratory tools, such as nuclear magnetic resonance techniques and chromatography, to both locate the molecule and identify its structure. The molecule turns out to be a kind of fatty acid.

"It's becoming clear that symbiotic relationships between plants, animals, and microbes are essential for the diversification of life and evolution of organisms," says Currie. "This is an example of a system where we have insights into the importance of the diversity of microbes. We believe that this type of mutualism is widespread."

In addition, the researchers suspect that this association represents a source of small molecules that can be used in medicine.

"This molecule is nature's anti-fungal," says Clardy, "and it looks like there are a lot of them."

This is particularly significant, since pathogenic fungal infections in people are a major health concern. These infections are often fatal, and at the moment, no reliable medications for them exist. Here, however, we have an example of an antibiotic successfully disabling a powerful fungi.

"This particular molecule is too unstable to be a viable candidate," says Clardy. "Still, we need to study how it kills fungi, learn the mechanisms. We can look into other bacterial genomes and investigate other anti-fungal processes."

Suspecting that this symbiotic dynamic is far more the rule than the exception, Clardy and Currie are investigating other insect species as well to see how universal this "story arc" is.


'/>"/>

Contact: David Cameron
david_cameron@hms.harvard.edu
617-432-0441
Harvard Medical School
Source:Eurekalert

Related biology news :

1. Healthy Minds Across America features 48 public forums on mental health research, Sept. 14
2. Bitter-tasting nectar and floral odors optimize outcrossing for plants
3. Cross fire from the brain makes patients tremble
4. Crossed (evolutionary) signals?
5. Centromeres cross over, a lot
6. Energy Crossroads conference April 30 and May 1 at Stanford University
7. Scientists find a fingerprint of evolution across the human genome
8. Shilatifard Lab sheds light on molecular machinery required for translation of histone crosstalk
9. Gene study supports single main migration across Bering Strait
10. A mechanism to explain biological cross-talk between 24-hour body cycle and metabolism
11. Research sheds new light on how diseases jump across species
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:5/12/2016)... DALLAS , May 12, 2016 ... has just published the overview results from the Q1 ... of the recent wave was consumers, receptivity to a ... wearables data with a health insurance company. ... choose to share," says Michael LaColla , CEO ...
(Date:5/3/2016)... 2016  Neurotechnology, a provider of high-precision biometric ... Biometric Identification System (ABIS) , a complete system ... ABIS can process multiple complex biometric transactions with ... fingerprint, face or iris biometrics. It leverages the ... MegaMatcher Accelerator , which have been used ...
(Date:4/28/2016)... First quarter 2016:   , ... the first quarter of 2015 The gross margin was ... 18.8) and the operating margin was 40% (-13) Earnings ... flow from operations was SEK 249.9 M (21.2) , ... SEK 7,000-8,500 M. The operating margin for 2016 is ...
Breaking Biology News(10 mins):
(Date:6/23/2016)... ... June 23, 2016 , ... Supplyframe, the ... the Supplyframe Design Lab . Located in Pasadena, Calif., the Design Lab’s ... how hardware projects are designed, built and brought to market. , The Design ...
(Date:6/23/2016)... ... , ... In a new case report published today in STEM CELLS Translational ... lymphedema after being treated for breast cancer benefitted from an injection of stem cells ... this debilitating, frequent side effect of cancer treatment. , Lymphedema refers to ...
(Date:6/23/2016)... 23, 2016  Blueprint Bio, a company dedicated to ... medical community, has closed its Series A funding round, ... "We have received a commitment from Forentis ... need to meet our current goals," stated Matthew ... runway to complete validation on the current projects in ...
(Date:6/23/2016)... 23, 2016 On Wednesday, June 22, ... down 0.22%; the Dow Jones Industrial Average edged 0.27% lower ... 2,085.45, down 0.17%. Stock-Callers.com has initiated coverage on the following ... Therapeutics (NASDAQ: NKTR ), Aralez Pharmaceuticals Inc. (NASDAQ: ... BIND ). Learn more about these stocks by accessing ...
Breaking Biology Technology: