Navigation Links
Cracking the plant-cell membrane code
Date:3/22/2010

Palo Alto, CATo engineer better, more productive crops and develop new drugs to combat disease, scientists look at how the sensor-laden membranes surrounding cells control nutrient and water uptake, secrete toxins, and interact with the environment and neighboring cells to affect growth and development. Remarkably little is known about how proteins interact with these protective structures. With National Science Foundation funding, researchers at the Carnegie Institution's Department of Plant Biology are using the first high-throughput screen for any multicellular organism to pinpoint these interactions using the experimental plant Arabidopsis. They have analyzed some 3.4 million potential protein/membrane interactions and have found 65,000 unique relationships. They made the preliminary data available today to the biological community by way of the Website www.associomics.org/search.php. Since proteins are similar in all organisms, the work is relevant to fields from farming to medicine.

"This is just the beginning," remarked Wolf Frommer director of Carnegie's Department of Plant Biology. "Arabidopsis shares many of its genes with other organisms including humans. As the library of interacting proteins grows, scientists around the world will be able to study the details of protein interactions to understand how they are affected by forces such as climate change and disease and how they can be harnessed to produce better crops and medicines more effectively."

All of a cell's internal machinery relies on the binding of proteins. Complementary shaped proteins dock with one another to start processes, such as turning on a gene or letting in the proper nutrient. These membrane proteins make up some 20-30% of the proteins in Arabidopsis, a relative of the mustard plant.

The team uses a screen called the mating-based protein complementation assay, or split ubiquitin system. Ubiquitin is a small protein. The scientists fuse candidate proteins onto a version of ubiquitin that is split in half. When the two candidates interact, the two halves of the ubiquitin reassemble, triggering a process that liberates a transcription factora protein that switches a gene onwhich then goes to the nucleus. When genes are turned on in the nucleus, the researchers are alerted to the successful interaction. The ultimate goal is to test the 36 million potential interactions as well as the sensitivity of the interactions to small molecules with a high-throughput robotics system.

The group plans to start a second round of screening at the end of this month to test another 3.4 million interactions.


'/>"/>

Contact: Wolf Frommer
wfrommer@CarnegieScience.edu
650-325-1521
Carnegie Institution
Source:Eurekalert

Related biology news :

1. Computers aid in cracking deception in plants
2. Sands of Gobi Desert yield new species of nut-cracking dinosaur
3. Cracking the species code for plants
4. Mussel-inspired glue for fetal membrane repair
5. Membrane-coat proteins: Bacteria have them too
6. Sticky protein helps reinforce fragile muscle membranes
7. How mitochondria get their membranes bent
8. Phytoplankton cell membranes challenge fundamentals of biochemistry
9. Improved technique determines structure in membrane proteins
10. GKSS membranes reduce air pollution in Beijing
11. New chlorine-tolerant, desalination membrane hopes to boost access to clean water
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/5/2017)... , April 5, 2017 Today HYPR ... that the server component of the HYPR platform is ... providing the end-to-end security architecture that empowers biometric authentication ... HYPR has already secured over 15 million users across ... manufacturers of connected home product suites and physical access ...
(Date:3/30/2017)... HONG KONG , March 30, 2017 ... developed a system for three-dimensional (3D) fingerprint identification by adopting ground ... technology into a new realm of speed and accuracy for use ... applications at an affordable cost. ... ...
(Date:3/24/2017)... March 24, 2017 The Controller General of Immigration ... Abdulla Algeen have received the prestigious international IAIR Award for ... Continue Reading ... ... Deputy Controller Abdulla Algeen (small picture on the right) have received the ...
Breaking Biology News(10 mins):
(Date:9/15/2017)... ... September 15, 2017 , ... San Diego based graphene materials ... B’ round of financing, totaling $600,000. The round was entirely filled by ... structure at a company valuation of $10M. , Grolltex, short for ...
(Date:9/14/2017)... ... ... Boston Strategic Partners, Inc. (BSP), a life-sciences and healthcare ... (HEOR) and ‘big data’ to provide in-depth analysis of pneumonia patients characteristics, medication ... with nearly 1/3 spent on hospitalizations. BSP has access to real-world data from ...
(Date:9/14/2017)... ... 14, 2017 , ... Cambridge Semantics , the leading ... its Anzo Smart Data Lake has been named a KMWorld Trend-Setting Product ... help organizations succeed in surpassing their knowledge management goals. KMWorld searches for the ...
(Date:9/12/2017)... (PRWEB) , ... September 12, 2017 , ... ... the fastest growing b2b product testing and development companies will be exhibiting at ... offerings. , Contract Pharma is an educational conference for ...
Breaking Biology Technology: