Navigation Links
Cracking the plant-cell membrane code
Date:3/22/2010

Palo Alto, CATo engineer better, more productive crops and develop new drugs to combat disease, scientists look at how the sensor-laden membranes surrounding cells control nutrient and water uptake, secrete toxins, and interact with the environment and neighboring cells to affect growth and development. Remarkably little is known about how proteins interact with these protective structures. With National Science Foundation funding, researchers at the Carnegie Institution's Department of Plant Biology are using the first high-throughput screen for any multicellular organism to pinpoint these interactions using the experimental plant Arabidopsis. They have analyzed some 3.4 million potential protein/membrane interactions and have found 65,000 unique relationships. They made the preliminary data available today to the biological community by way of the Website www.associomics.org/search.php. Since proteins are similar in all organisms, the work is relevant to fields from farming to medicine.

"This is just the beginning," remarked Wolf Frommer director of Carnegie's Department of Plant Biology. "Arabidopsis shares many of its genes with other organisms including humans. As the library of interacting proteins grows, scientists around the world will be able to study the details of protein interactions to understand how they are affected by forces such as climate change and disease and how they can be harnessed to produce better crops and medicines more effectively."

All of a cell's internal machinery relies on the binding of proteins. Complementary shaped proteins dock with one another to start processes, such as turning on a gene or letting in the proper nutrient. These membrane proteins make up some 20-30% of the proteins in Arabidopsis, a relative of the mustard plant.

The team uses a screen called the mating-based protein complementation assay, or split ubiquitin system. Ubiquitin is a small protein. The scientists fuse candidate proteins onto a version of ubiquitin that is split in half. When the two candidates interact, the two halves of the ubiquitin reassemble, triggering a process that liberates a transcription factora protein that switches a gene onwhich then goes to the nucleus. When genes are turned on in the nucleus, the researchers are alerted to the successful interaction. The ultimate goal is to test the 36 million potential interactions as well as the sensitivity of the interactions to small molecules with a high-throughput robotics system.

The group plans to start a second round of screening at the end of this month to test another 3.4 million interactions.


'/>"/>

Contact: Wolf Frommer
wfrommer@CarnegieScience.edu
650-325-1521
Carnegie Institution
Source:Eurekalert

Related biology news :

1. Computers aid in cracking deception in plants
2. Sands of Gobi Desert yield new species of nut-cracking dinosaur
3. Cracking the species code for plants
4. Mussel-inspired glue for fetal membrane repair
5. Membrane-coat proteins: Bacteria have them too
6. Sticky protein helps reinforce fragile muscle membranes
7. How mitochondria get their membranes bent
8. Phytoplankton cell membranes challenge fundamentals of biochemistry
9. Improved technique determines structure in membrane proteins
10. GKSS membranes reduce air pollution in Beijing
11. New chlorine-tolerant, desalination membrane hopes to boost access to clean water
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/24/2017)... 2017 Research and Markets has announced the ... & Trends - Industry Forecast to 2025" report to their ... The Global ... CAGR of around 15.1% over the next decade to reach approximately ... the market estimates and forecasts for all the given segments on ...
(Date:3/22/2017)... March 21, 2017 Optimove , ... by retailers such as 1-800-Flowers and AdoreMe, today ... Recommendations and Replenishment. Using Optimove,s machine learning algorithms, ... product and replenishment recommendations to their customers based ... predictions of customer intent drawn from a complex ...
(Date:3/16/2017)... Against identity fraud with DERMALOG solutions "Made in Germany "  ... ... solutions provide a crucial contribution against identity fraud. (PRNewsFoto/Dermalog Identification Systems) ... Used combined in one project, multi-biometric solutions provide a crucial contribution against identity fraud. ... ...
Breaking Biology News(10 mins):
(Date:4/20/2017)... ... , ... USDM Life Sciences , the leading risk management, technological innovation ... to announce Holger Braemer as Vice President of its Europe division and ... , Braemer is an integral part of USDM’s expansion of services and solutions ...
(Date:4/20/2017)... (PRWEB) , ... April 20, 2017 , ... NetDimensions appoints ... Sales. , With over 20 years of experience in the learning technologies industry, Mastin ... sister company within Learning Technologies Group plc (LTG). At LEO, Mastin served as SVP ...
(Date:4/20/2017)... ... 20, 2017 , ... Energetiq Technology , a world ... Chief Executive Officer (CEO) Debbie Gustafson has been appointed to the SEMI North ... connecting the electronics manufacturing supply chain. The mission of the SEMI NAAB is ...
(Date:4/20/2017)... ... April 20, 2017 , ... Open Therapeutics and the ... sharing and commercialization model. , The Center for Advancing Innovation helps institutions maximize ... effort is bringing the IP to the attention of the entrepreneurial community and ...
Breaking Biology Technology: