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Cow stomach holds key to turning corn into biofuel
Date:4/8/2008

turally occurring fungus, takes the large cellulose pieces created by the first enzyme and breaks them into sugar pairs.

Spartan Corn III, with the gene from a microbe in a cow, produces an enzyme that separates pairs of sugar molecules into simple sugars. These single sugars are readily fermentable into ethanol, meaning that when the cellulose is in simple sugars, it can be fermented to make ethanol.

It will save money in ethanol production, Sticklen said. Without it they cant convert the waste into ethanol without buying enzymes which is expensive.

The Spartan Corn line was created by inserting an animal stomach microbe gene into a plant cell. The DNA assembly of the animal stomach microbe required heavy modification in the lab to make it work well in the corn cells. Sticklen compared the process to adding a single Christmas tree light to a tree covered in lights.

You have a lot of wiring, switches and even zoning, Sticklen said. There are a lot of changes. We have to increase production levels and even put it in the right place in the cell.

If the cell produced the enzyme in the wrong place, then the plant cell would not be able to function, and, instead, it would digest itself. That is why Sticklen found a specific place to insert the enzyme.

One of the targets for the enzyme produced in Spartan Corn III is a special part of the plant cell, called the vacuole. The vacuole is a safe place to store the enzyme until the plant is harvested. The enzyme will collect in the vacuole with other cellular waste products

Because it is only in the vacuole of the green tissues of plant cells, the enzyme is only produced in the leaves and stalks of the plant, not in the seeds, roots or the pollen. It is only active when it is being used for biofuels because of being stored in the vacuole

Spartan Corn III is one step ahead for science, technology, and it is even a step politically, Sticklen said. It
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Contact: Tom Oswald
oswald@msu.edu
517-432-0920
Michigan State University
Source:Eurekalert  

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