Navigation Links
Controlling gene expression with hydrogen peroxide 'switches'
Date:8/1/2012

Hydrogen peroxide doesn't just come in bottles from the drugstore the human body makes it as well. Now researchers from North Carolina State University have found a way to use naturally occurring hydrogen peroxide inside cells to switch on gene expression. Their method also serves as a highly sensitive hydrogen peroxide detector, which may help scientists determine the molecule's role in cellular health and disease.

In a normally functioning cell, hydrogen peroxide serves as a messenger, carrying signals through a cell in order to allow the cell to respond to external stimuli or events. Once the message is transmitted, the hydrogen peroxide diffuses and disappears.

"It's an ideal messenger compound, because it's small, fast, and doesn't linger," says chemist Alex Deiters. "It works by oxidizing, or modifying, certain amino acids in proteins, which affects the protein's function."

Deiters and graduate students Jeane Govan, Andrew McIver and Chad Riggsbee wanted to see if they could harness hydrogen peroxide's oxidizing property as a way to control gene expression, using the gene that gives fireflies their "glow," or luminescence, as a test case. They designed a molecule that was sensitive to hydrogen peroxide and enabled expression of the firefly luciferase gene in live mammalian cells. When hydrogen peroxide was present, the luciferase gene was expressed, causing the cell to glow.

The researchers' results appear online in Angewandte Chemie.

"The exciting aspects of this synthetic gene switch are that you can use it not only as a way to detect the presence of hydrogen peroxide within cells, in this case, by making cells containing hydrogen peroxide glow, but also to express any gene of interest in response to the presence of hydrogen peroxide," Deiters says. "In terms of detecting hydrogen peroxide, the system is at least an order of magnitude more sensitive than anything used previously."


'/>"/>
Contact: Tracey Peake
tracey_peake@ncsu.edu
919-515-6142
North Carolina State University
Source:Eurekalert

Page: 1

Related biology news :

1. Controlling inflammatory and immune responses
2. IU biologists offer clearer picture of how protein machine systems tweak gene expression
3. A new application allows online statistical analysis of gene-expression data
4. Measuring progesterone receptor expression to improve hormone-receptor-positive cancer management
5. Fish show autism-like gene expression in water with psychoactive pharmaceuticals
6. Low-cost nanosheet catalyst discovered to split hydrogen from water
7. Finished heart switches stem cells off
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/15/2016)... 15, 2016 Research and ... Biometrics Market 2016-2020,"  report to their offering.  , ... , ,The global gait biometrics market is expected ... the period 2016-2020. Gait analysis generates ... be used to compute factors that are not ...
(Date:3/31/2016)... 31, 2016   ... the "Company") LegacyXChange is excited to release ... soon to be launched online site for trading 100% ... ) will also provide potential shareholders a sense of ... to an industry that is notorious for fraud. The ...
(Date:3/23/2016)... 2016 Einzigartige ... und Stimmerkennung mit Passwörtern     ... MESG ), ein führender Anbieter digitaler Kommunikationsdienste, ... SpeechPro zusammenarbeitet, um erstmals dessen Biometrietechnologie einzusetzen. ... Möglichkeit angeboten, im Rahmen mobiler Apps neben ...
Breaking Biology News(10 mins):
(Date:6/23/2016)... LOUISVILLE, Ky. , June 23, 2016 /PRNewswire/ ... from two Phase 1 clinical trials of its ... double-blind, placebo-controlled, single and multiple ascending dose studies ... and pharmacodynamics (PD) of subcutaneous injection in healthy ... APL-2 subcutaneously (SC) either as a single dose ...
(Date:6/23/2016)... June 23, 2016 On Wednesday, June ... 4,833.32, down 0.22%; the Dow Jones Industrial Average edged 0.27% ... at 2,085.45, down 0.17%. Stock-Callers.com has initiated coverage on the ... Nektar Therapeutics (NASDAQ: NKTR ), Aralez Pharmaceuticals Inc. ... BIND ). Learn more about these stocks by ...
(Date:6/23/2016)... ... June 23, 2016 , ... Velocity ... intelligent tools designed, tuned and optimized exclusively for Okuma CNC machining centers at ... result of a collaboration among several companies with expertise in toolholding, cutting tools, ...
(Date:6/22/2016)... Cell Applications, Inc. and StemoniX announced ... up to one billion human induced pluripotent stem ... These high-quality, consistent stem cells enable researchers to ... more time doing meaningful, relevant research. This achievement ... process that produces affordable, reliable HiPSC for life ...
Breaking Biology Technology: