Navigation Links
Controlling gene expression with hydrogen peroxide 'switches'
Date:8/1/2012

Hydrogen peroxide doesn't just come in bottles from the drugstore the human body makes it as well. Now researchers from North Carolina State University have found a way to use naturally occurring hydrogen peroxide inside cells to switch on gene expression. Their method also serves as a highly sensitive hydrogen peroxide detector, which may help scientists determine the molecule's role in cellular health and disease.

In a normally functioning cell, hydrogen peroxide serves as a messenger, carrying signals through a cell in order to allow the cell to respond to external stimuli or events. Once the message is transmitted, the hydrogen peroxide diffuses and disappears.

"It's an ideal messenger compound, because it's small, fast, and doesn't linger," says chemist Alex Deiters. "It works by oxidizing, or modifying, certain amino acids in proteins, which affects the protein's function."

Deiters and graduate students Jeane Govan, Andrew McIver and Chad Riggsbee wanted to see if they could harness hydrogen peroxide's oxidizing property as a way to control gene expression, using the gene that gives fireflies their "glow," or luminescence, as a test case. They designed a molecule that was sensitive to hydrogen peroxide and enabled expression of the firefly luciferase gene in live mammalian cells. When hydrogen peroxide was present, the luciferase gene was expressed, causing the cell to glow.

The researchers' results appear online in Angewandte Chemie.

"The exciting aspects of this synthetic gene switch are that you can use it not only as a way to detect the presence of hydrogen peroxide within cells, in this case, by making cells containing hydrogen peroxide glow, but also to express any gene of interest in response to the presence of hydrogen peroxide," Deiters says. "In terms of detecting hydrogen peroxide, the system is at least an order of magnitude more sensitive than anything used previously."


'/>"/>
Contact: Tracey Peake
tracey_peake@ncsu.edu
919-515-6142
North Carolina State University
Source:Eurekalert

Page: 1

Related biology news :

1. Controlling inflammatory and immune responses
2. IU biologists offer clearer picture of how protein machine systems tweak gene expression
3. A new application allows online statistical analysis of gene-expression data
4. Measuring progesterone receptor expression to improve hormone-receptor-positive cancer management
5. Fish show autism-like gene expression in water with psychoactive pharmaceuticals
6. Low-cost nanosheet catalyst discovered to split hydrogen from water
7. Finished heart switches stem cells off
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/11/2017)... DUBLIN , Apr. 11, 2017 Research ... Tracking Market 2017-2021" report to their offering. ... The global eye tracking market to grow at ... The report, Global Eye Tracking Market 2017-2021, has been prepared based ... report covers the market landscape and its growth prospects over the ...
(Date:4/11/2017)... 11, 2017 NXT-ID, Inc. (NASDAQ:   ... announces the appointment of independent Directors Mr. Robin D. ... Board of Directors, furthering the company,s corporate governance and expertise. ... Gino Pereira , ... forward to their guidance and benefiting from their considerable expertise ...
(Date:4/5/2017)... Allen Institute for Cell Science today announces the launch ... dynamic digital window into the human cell. The website ... deep learning to create predictive models of cell organization, ... suite of powerful tools. The Allen Cell Explorer will ... resources created and shared by the Allen Institute for ...
Breaking Biology News(10 mins):
(Date:10/9/2017)... ... , ... At its national board meeting in North Carolina, ARCS® Foundation ... of Physics and Astronomy, has been selected for membership in ARCS Alumni Hall ... 2015 Breakthrough Prize in Fundamental physics for the discovery of the accelerating expansion of ...
(Date:10/7/2017)... (PRWEB) , ... October 06, ... ... years’ experience providing advanced instruments and applications consulting for microscopy and surface ... expertise in application consulting, Nanoscience Analytical offers a broad range of contract ...
(Date:10/6/2017)... ... October 06, 2017 , ... The HealthTech ... technology sector at their fourth annual Conference where founders, investors, innovative practitioners and ... the ELEVATE pitch competition showcasing early stage digital health and med tech companies. ...
(Date:10/5/2017)... Philadelphia, PA (PRWEB) , ... October 05, 2017 , ... ... of the newest frontiers in human health. Gut Love: You Are My Future, the ... offers an artist’s perspective as it explores the human condition through the lens of ...
Breaking Biology Technology: