Navigation Links
Consortium develops new method enabling routine targeted gene modification
Date:7/24/2008

A multi-institutional team led by Massachusetts General Hospital (MGH) investigators has developed a powerful new tool for genomic research and medicine a robust method for generating synthetic enzymes that can target particular DNA sequences for inactivation or repair. In the July 25 issue of Molecular Cell, the researchers describe an efficient, publicly available method to engineer customized zinc-finger nucleases (ZFNs), which can be used to induce specific genomic modifications in many types of cells.

"Recent work has shown that ZFNs can alter genes with high efficiency in cells from plants or model organisms like fruitflies, roundworms and zebrafish, and in human cells," says J. Keith Joung, MD, PhD, of the MGH Molecular Pathology Unit, the paper's senior author. "However, a significant bottleneck has been the lack of access to an effective method for generating the customized DNA-binding domains needed to guide ZFNs to their target sites. Our method will enable academic researchers to rapidly create high quality ZFNs for genes of interest and will stimulate use of this technology in biological research and potentially gene therapy."

Zinc-finger peptides, which bind to DNA, occur naturally in many important proteins that regulate or otherwise interact with DNA. Zinc-finger nucleases are constructed from synthetic "designer" zinc-finger domains targeted to a specific genetic sequence and another protein segment that breaks both DNA strands within the binding site. Currently available methods for generating ZFNs are either inefficient or involve constructing and analyzing huge libraries of zinc-finger peptides, a task that exceeds the capabilities of all but a handful of laboratories in the world.

First author Morgan L. Maeder of the Joung lab led an effort by researchers from six institutions that demonstrated how this new method (termed OPEN for Oligomerized Pool ENgineering) can rapidly generate ZFNs that induc
'/>"/>

Contact: Sue McGreevey
smcgreevey@partners.org
617-724-2764
Massachusetts General Hospital
Source:Eurekalert

Page: 1 2

Related biology news :

1. Midlands Consortium delighted to be chosen to host £1 billion energy institute
2. NIH expands national consortium dedicated to transforming clinical and translational research
3. Consortium develops new method to manipulate genetic material
4. How schizophrenia develops: Major clues discovered
5. MIT develops tractor beam for cells, more
6. Team of scientists develops non-invasive method to track nerve-cell development in live human brain
7. MBL develops infrastructure and portal for Encyclopedia of Life
8. U of I researcher develops power-packed soy breakfast cereal
9. Scientists discover how TB develops invincibility against only available treatment
10. Europe develops new technologies to boost health of livestock
11. Queens develops safe green decontamination method
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/24/2015)... Fla. , March 24, 2015   ... management solutions, today announced that its U.are.U ® ... accessory retailer Goorin Bros . to achieve ... biometrics-based solution secures access to the POS terminal, ... increased risk of data breaches. ...
(Date:3/20/2015)... , Mar. 20, 2015 Research and Markets ... addition of the "India Sensors Market Forecast ... The sensor market is projected to ... Consumer electronics, automotive, industrial and healthcare ... in the country. In addition, adoption of MEMS ...
(Date:3/18/2015)... -- As mobile payments become more prevalent amongst ... apps continue to be introduced into the market place at ... industry in focus today are:  NXT-ID, Inc. (NASDAQ: ... ), Apple Inc. (NASDAQ: AAPL ), Google Inc. ... FB ) NXT-ID, Inc. (NASDAQ: ...
Breaking Biology News(10 mins):Crossmatch Fingerprint Readers Help Retailer Prevent Data Breaches at Point-of-Sale 2India Sensors Market Forecast and Opportunities 2020 - STMicroelectronics held the largest share in 2014 2India Sensors Market Forecast and Opportunities 2020 - STMicroelectronics held the largest share in 2014 3Revolutionary Smart Wallet & Mobile Payment Choices Change the Game for Consumers - Company Begins Shipping Newest Digital Wallet to Customers 2Revolutionary Smart Wallet & Mobile Payment Choices Change the Game for Consumers - Company Begins Shipping Newest Digital Wallet to Customers 3Revolutionary Smart Wallet & Mobile Payment Choices Change the Game for Consumers - Company Begins Shipping Newest Digital Wallet to Customers 4Revolutionary Smart Wallet & Mobile Payment Choices Change the Game for Consumers - Company Begins Shipping Newest Digital Wallet to Customers 5Revolutionary Smart Wallet & Mobile Payment Choices Change the Game for Consumers - Company Begins Shipping Newest Digital Wallet to Customers 6
... consequences of iron deficiencies is the fact that too ... of the genetic iron overload disorder hereditary hemochromatosis ... from the European Molecular Biology Laboratory (EMBL) and the ... is a liver disease. They report in the current ...
... the world stand to benefit from a new research ... that aims to develop more advanced fertilizers for ... Research Centre, to be based at the University of ... The new centre has been established by the ...
... NEW YORK CITY - Dr. Marie-Jolle Rochet, a research ... of the Sea) has been awarded the prestigious Pew ... commercial fishing approaches is least harmful to the delicately ... urge international officials to encourage widespread use of that ...
Cached Biology News:Europe's most common genetic disease is a liver disorder 2Fertilizer research center an Australian first 22008 Pew Fellowship in Marine Conservation awarded to Dr. Marie-Joëlle Rochet from IFREMER 22008 Pew Fellowship in Marine Conservation awarded to Dr. Marie-Joëlle Rochet from IFREMER 32008 Pew Fellowship in Marine Conservation awarded to Dr. Marie-Joëlle Rochet from IFREMER 4
(Date:3/26/2015)... , March 26, 2015 ... a global clinical research organization , ... a definitive agreement to acquire all of ... ("QSI"), a leading provider of specialized pharmacovigilance ... to the collection, detection, assessment, monitoring, and ...
(Date:3/25/2015)... of the most attractive in the emerging European region, ... economy and increasing government investment in healthcare, according to ... is set to grow at twice the pace of ... annually reaching an approximate market value of $43-60 billion ... Green Cross provides total healthcare solutions that address the ...
(Date:3/25/2015)... , March 25, 2015  S&P Capital ... Factual Stock Report coverage on Abattis Bioceuticals ... ATTBF; CSE: ATT): is a specialty biotechnology company ... cultivating, licensing and marketing proprietary ingredients, bio-similar compounds, ... in North America . The ...
(Date:3/25/2015)... SPRINGS, Fla. , March 25, 2015 /PRNewswire/ ... strategy and management consulting firm, has launched the ... publication to simplify tracking of ePrescribing regulatory requirements ... "The ePrescribing regulatory landscape continues to change ... to deliver what the industry, and ultimately patients, ...
Breaking Biology Technology:PAREXEL Announces Execution of Definitive Agreement to Acquire Quantum Solutions India, Strengthening Leadership In Pharmacovigilance Services 2PAREXEL Announces Execution of Definitive Agreement to Acquire Quantum Solutions India, Strengthening Leadership In Pharmacovigilance Services 3PAREXEL Announces Execution of Definitive Agreement to Acquire Quantum Solutions India, Strengthening Leadership In Pharmacovigilance Services 4S&P Capital IQ Initiates Coverage on Abattis Bioceuticals Corp. in S&P Capital IQ Factual Stock Reports 2S&P Capital IQ Initiates Coverage on Abattis Bioceuticals Corp. in S&P Capital IQ Factual Stock Reports 3S&P Capital IQ Initiates Coverage on Abattis Bioceuticals Corp. in S&P Capital IQ Factual Stock Reports 4S&P Capital IQ Initiates Coverage on Abattis Bioceuticals Corp. in S&P Capital IQ Factual Stock Reports 5Point-of-Care Partners Launches the ePrescribing State Law Capsule 2
... 20, 2010 ... ... --> ... // Results are keyed by longUrl, so we need to grab the first one. for (var r in data.results) ...
... SAN DIEGO, Oct. 19, 2010 Amylin Pharmaceuticals, Inc. ... net product sales for the quarter ended September 30, ... the quarter include $132.4 million for BYETTA® (exanatide) injection ... compares to net product sales of $192.9 million consisting ...
... 19, 2010 -- The Cold Triple Axis spectrometer, a ... Isotope Reactor and a complementary tool to other neutron ... The CTAX uses "cold" neutrons from the HFIR ... Cold neutrons are slower than their "thermal" neutron counterparts, ...
Cached Biology Technology:JIPH Accepted for MEDLINE Indexing 2JIPH Accepted for MEDLINE Indexing 3JIPH Accepted for MEDLINE Indexing 4JIPH Accepted for MEDLINE Indexing 5JIPH Accepted for MEDLINE Indexing 6JIPH Accepted for MEDLINE Indexing 7JIPH Accepted for MEDLINE Indexing 8JIPH Accepted for MEDLINE Indexing 9ORNL's research reactor revamps veteran neutron scattering tool 2
... Novexin offers bespoke, cost-effective solutions for complex protein processing ... expertise and experience to help you develop a solution ... , ... transfer all methodology to ...
... 'Agilents GeneSpring Workgroup solution is ... managing and mining expression data in ... and is deployed in over 40 ... research organizations worldwide. GeneSpring Workgroup streamlines ...
... Sciences provides a genome-wide microRNA (miRNA) expression ... probe design, which enable highly sensitive and ... have standard arrays for mature miRNA of ... of the Sanger miRBase database (Release 8.2). ...
... ProXPRESS 2D Proteomic Imaging System, Basic Package ... a Wide Dynamic Range: The ProXPRESS 2D ... in imaging system for both proteomic and ... provides unparalleled flexibility, enabling the use of ...
Biology Products: