Navigation Links
Comprehensive understanding of bacteria could lead to new insights into many organisms
Date:9/25/2009

Investigators at Burnham Institute for Medical Research (Burnham), University of California, San Diego (UC San Diego), The Scripps Research Institute (TSRI), Genomics Institute of the Novartis Research Foundation (GNF) and other institutions have constructed a complete model, including three dimensional protein structures, of the central metabolic network of the bacterium Thermotoga maritima (T. maritima). This is the first time scientists have developed such a comprehensive model of a metabolic network overlaid with an atomic resolution of network proteins. The analysis of the model, among others, highlights the important role of a small number of essential protein shapes, lending new insights into the evolution of protein networks and the functions within these networks. The study was published in the journal Science on September 18.

Combining biochemical studies, structural genomics and computer modeling, the researchers deciphered the shapes, functions and interactions of 478 proteins that make up T. maritima's central metabolism. The team also found connections between these proteins and 503 unique metabolites in 562 intracellular and 83 extracellular metabolic reactions.

"We have built an actual three dimensional model of every protein in the central metabolic system," said Adam Godzik, Ph.D., director of Burnham's Bioinformatics and Systems Biology program. "We got the whole thing. This is analogous to sequencing an entire genome."

With this data, scientists can simulate metabolism simultaneously on a biochemical and molecular level. This information has the promise to expand computer modeling to allow investigators to simulate the interactions between proteins and various compounds in an entire system. Furthermore, the procedure developed in this study could be applied to study many other organisms, including humans. It could potentially help identify both positive and adverse drug reactions before pre-clinical and clinical trials. The research may also have applications in energy research, as bacteria like T. maritima can be engineered to more efficiently produce hydrogen, a key source of clean energy.

"In addition to the systematic analysis of interacting components, the next challenge is addressing the levels or scales of biological organization, ranging from molecules to an individual and even to populations," said co-author John C. Wooley, Ph.D., of UC San Diego's Center for Research in Biological Systems and the California Institute for Telecommunications and Information Technology. "This work, by including both functional and architectural details, takes that first step and provides a novel, enriched view of the complexity of life."

Researchers were surprised by the degree of structural conservation within the network. Of the 478 proteins, with 714 domains, there were only 182 distinct folds. This supports the hypothesis that nature uses existing shapes, slightly modified, to perform new tasks.

The team used genomic, metabolic, and structural reconstruction to determine the network down to the atomic level. They then classified metabolic reactions based on whether they were similar, connected or unrelated and found that enzymes that catalyze similar reactions have a higher probability of having similar folds. In addition, using a reductive evolution simulation approach, they uncovered the absolutely essential proteins to support a minimal viable network.

"We were able to put together the information from the network biology as well as the protein structural biology," said co-author Bernhard Palsson, Ph.D., a UC San Diego bioengineering professor who leads the Systems Biology Research Group. "This is the first time this has been accomplished. We are in a position to study microorganisms in much greater detail, including those that are important in health care and those that are of environmental concern."


'/>"/>

Contact: Andrea Siedsma
asiedsma@soe.ucsd.edu
858-822-0899
University of California - San Diego
Source:Eurekalert  

Related biology news :

1. Leading researchers to reveal comprehensive dos and donts for prostate cancer
2. University of Minnesota releases first ever comprehensive report of the health of college students
3. More comprehensive analysis of Klamath River basin needed to aid decision makers
4. U. of I. librarian launches comprehensive Web database of field guides
5. Scientists launch first comprehensive database of human oral microbiome
6. Collaboration between researchers yields more comprehensive portrait of brain cancer
7. First comprehensive genomic study of common cold reveals new treatment targets
8. First comprehensive inventory of life in Antarctica
9. Scientists develop method for comprehensive proteome analysis
10. Comprehensive genetic study paves way for new blood-pressure medicines
11. Uniloc Unveils Industry-First Authentication Technology for Comprehensive Protection of Critical Infrastructure
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Comprehensive understanding of bacteria could lead to new insights into many organisms
(Date:6/20/2016)... 20, 2016 Securus Technologies, a leading ... for public safety, investigation, corrections and monitoring announced ... it has secured the final acceptance by all ... Managed Access Systems (MAS) installed. Furthermore, Securus will ... be installed by October, 2016. MAS distinguishes between ...
(Date:6/9/2016)... innovation leader in attendance control systems is proud to announce the introduction of fingerprint ... sure the right employees are actually signing in, and to even control the opening ... ... ... Photo ...
(Date:6/3/2016)... , June 3, 2016 ... Management) von Nepal ... und Lieferung hochsicherer geprägter Kennzeichen, einschließlich Personalisierung, ... führend in der Produktion und Implementierung von ... der Ausschreibung im Januar teilgenommen, aber Decatur ...
Breaking Biology News(10 mins):
(Date:12/2/2016)... ... December 01, 2016 , ... ... Symposium (CSS) and the popularity of US Single Day Events (SDE) to organize ... early Summer 2018, in Raleigh, NC. Topics of the pharmaceutical and life sciences ...
(Date:12/2/2016)... CHICAGO, IL (PRWEB) , ... December 01, 2016 ... ... pleased to congratulate long-term client Nanowear on their recent FDA Class II 510(k) ... has cleared a significant hurdle in commercializing remote cardiac monitoring devices that rely ...
(Date:12/2/2016)... --  SurePure, Inc. (OTCQB: SURP) a global leader ... concluded an agreement with Tamarack Biotics under which Tamarack ... acquire units of the Company,s patented photopurification technology with ... Concurrently with the option, SurePure has entered ... seek regulatory approvals in the United States ...
(Date:11/30/2016)... ... 30, 2016 , ... BEI Kimco, a brand of Sensata ... design that ensures high alignment accuracy by preventing unwanted shaft rotation. The new ... extreme precision is required, such as in medical equipment, laboratory instrumentation, clean rooms, ...
Breaking Biology Technology: