Navigation Links
Combined molecular study techniques reveal more about DNA proteins
Date:3/2/2011

CHAMPAIGN, Ill. Illinois researchers have combined two molecular imaging technologies to create an instrument with incredible sensitivity that provides new, detailed insight into dynamic molecular processes.

Physics professors Taekjip Ha and Yann Chemla and combined their expertise in single-molecule biophysics fluorescence microscopy and optical traps, respectively to study binding and unbinding of individual DNA segments to a larger strand. They and their joint postdoctoral researcher Matthew Comstock detail their technique in a paper published in the Feb. 20 online edition of Nature Methods.

Both professors have particularly studied proteins and enzymes that regulate DNA, such as the enzyme helicase that unwinds DNA for duplication or transcription to RNA. Fluorescent microscopy techniques allow researchers to observe proteins as they conform and move, but often lack the spatial range to track the protein's motion over distance.

Optical traps, meanwhile, enable researchers to study a protein's translocation, but not its conformation. Chemla compares traditional optical traps to fishing. A single molecule of DNA is tethered between two attachment points, and the activity of a protein bound to it is only inferred from how it tugs on the tether, much like a fish at the end of a line. This can reveal a lot about a protein's activity and motion, but the technique has glaring limitations as well. For example, it is difficult to know how many proteins or the types of proteins that are involved.

"Also, these proteins may do all sorts of things beyond tugging on our line that we may not be sensitive to," Chemla said. "Fluorescence allows you to have an additional readout to actually see these things, and the key is that we can now measure them simultaneously. This work was a real synthesis of the expertise of two groups at the Center for the Physics of Living Cells at the U. of I."

The combination allows Chemla,
'/>"/>

Contact: Liz Ahlberg
eahlberg@illinois.edu
217-244-1073
University of Illinois at Urbana-Champaign
Source:Eurekalert

Page: 1 2

Related biology news :

1. Newborn screening increases survival outcome for patients with severe combined immunodeficiency
2. AOptix Technologies to Show Combined Biometric Face and Iris Capture System at Biometrics Consortium Conference
3. Reading food labels, combined with exercise, can lead to weight loss
4. Combined stem cell-gene therapy approach cures human genetic disease in vitro
5. A combined tooth-venom arsenal revealed as key to Komodo dragons hunting strategy
6. Fingerprints match molecular simulations with reality
7. Designing new molecular tools to study the life and death of a cancer cell
8. Molecular network influences development of chronic lymphocytic leukemia
9. Polymer membranes with molecular-sized channels that assemble themselves
10. Molecular medicine could avert predicted catastrophic vision loss in the aged
11. New book highlights the cellular and molecular determinants of brain wiring
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:7/9/2014)... geographic ranges in which animals can be found, concern ... Does an increased range for a host mean new ... says a team of UC Santa Barbara scientists, including ... published in the Journal of Biogeography , Hechinger, ... opposite may happen: Hosts may actually lose their parasites ...
(Date:7/9/2014)... (July 9, 2014) Emerging fungal pathogens pose ... parasitic group, causing population declines of amphibians, bats, ... University of South Florida published in the prestigious ... acquire behavioral or immunological resistance to a deadly ... , "Acquired resistance is important because it ...
(Date:7/9/2014)... injuries have identified an immune marker that predicts ... infection. The study, led by clinician-scientists at Nationwide ... the journal Shock , is part of ... clinical implementation of quick-turnaround immune function tests and ... following critical illness or injury in pediatric patients. ...
Breaking Biology News(10 mins):Not at home on the range 2Not at home on the range 3Not at home on the range 4USF study: Amphibians can acquire resistance to deadly fungus 2Immune function predicts infection risk among child trauma patients 2Immune function predicts infection risk among child trauma patients 3
... stem cells, ability to regenerate damaged tissue could offer ... myopathy and muscular dystrophy, according to researchers from the ... procedure has been successfully used to regrow muscles in ... all tissue-based illnesses in humans such as in the ...
... young, apparently healthy athletes suddenly collapse, it can be due ... have now discovered a genetic modification that leads to cardiac ... can give zebrafish a heart condition. Since the fish have ... could be critical for humans as well. ...
... and Innovation in Global BiometricsGAITHERSBURG, Md., March 4 ... has been named the Biometrics Systems Integrator "Company ... the Corporation,s leadership in developing innovative biometrics solutions ... Sullivan based the award on a rigorous analysis ...
Cached Biology News:Stem cell breakthrough gives new hope to sufferers of muscle-wasting diseases 2Why 'lazy Susan' has a weak heart 2Lockheed Martin Named Biometrics 'Company of the Year' by Frost and Sullivan 2
(Date:1/15/2014)... 15, 2014 TaiGen Biotechnology Company, Limited ("TaiGen") today ... R-Pharm, a leading Russian pharmaceutical company, to develop and ... Russian Federation , Turkey ... is a novel antibiotic for the treatment of bacterial ...
(Date:1/14/2014)... 2014  3D Communications, a leading provider of strategic communications services to ... events in the United States and ... Cox , JD, is returning to the firm,s Washington, ... 3D after more than two years of service as Associate Commissioner ...
(Date:1/14/2014)... SAN DIEGO and BETHESDA, Md. ... they are joining together with two institutes from the ... more reliable tools for bringing safer, more effective treatments ... with the National Center for Advancing Translational Sciences (NCATS) ...
(Date:1/14/2014)... Rockville, MD (PRWEB) January 14, 2014 ... dedicated to developing innovative information technology solutions for ... other health care stakeholders, announced today the signing ... the US Food and Drug Administration (FDA). ...
Breaking Biology Technology:TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 2TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 3TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 4Former FDA Associate Commissioner Returns To 3D Communications 2Organovo Announces Collaboration with National Institutes of Health 2Organovo Announces Collaboration with National Institutes of Health 3Global Record Systems Announces Research Collaboration Agreement with FDA to Create a Novel “Big Data” Paradigm for Collection of Patient Safety and Outcomes Information 2
... Dendreon Corporation (Nasdaq: DNDN ) today announced that management ... Healthcare Conference in New York City, NY on May 25, 2011 ... in Rancho Palos Verdes, CA on June 7, 2011 at 2:00 ... live and available for replay from Dendreon,s website, www.dendreon.com . ...
... BRISBANE, Calif., May 19, 2011 InterMune, Inc. (NASDAQ: ... of its 2011 Analyst Day presentations on Thursday, May ... in New York.  Presentations by InterMune,s executive management team ... and a comprehensive update of InterMune,s commercial and scientific ...
... CARY, N.C., May 19, 2011 ... its flexible 100% web-based Life Science Study Management ... successfully used globally by Leading European and USA ... eStudy,s on-line study design, management, communication, data collection ...
Cached Biology Technology:InterMune to Webcast 2011 Analyst Day Presentations 2Leading Ag Biotechnology Companies in Europe and America Save 50-80% of Field Study Management Time with eStudy 2Leading Ag Biotechnology Companies in Europe and America Save 50-80% of Field Study Management Time with eStudy 3
...
... An entire pressurized perfusion system ... in physiology, biophysics, electrochemistry, or general ... as little as 100 microliters of ... eight solutions in milliseconds from a ...
...
Request Info...
Biology Products: