Navigation Links
Columbia researchers report novel approach for single molecule electronic DNA sequencing
Date:9/21/2012

for Single Molecule DNA Sequencing by Synthesis" is now available in the open access online journal, Scientific Reports (2, 684 DOI:10.1038/srep00684, 2012), from the Nature Publication group.

The reported nanopore-based sequencing by synthesis (Nano-SBS) strategy can accurately distinguish four DNA bases by detecting 4 different sized tags released from 5'-phosphate-modified nucleotides at the single molecule level for sequence determination. The basic principle of the Nano-SBS strategy is described as follows. As each nucleotide analog is incorporated into the growing DNA strand during the polymerase reaction, its tag is released by phosphodiester bond formation. The tags will enter a nanopore in the order of their release, producing unique ionic current blockade signatures due to their distinct chemical structures, thereby determining DNA sequence electronically at single molecule level with single base resolution. As proof-of-principle, the research team attached four different length polymer tags to the terminal phosphate of 2'-deoxyguanosine-5'-tetraphosphate (a modified DNA building block) and demonstrated efficient incorporation of the nucleotide analogs during the polymerase reaction, as well as better than baseline discrimination among the four tags at single molecule level based on their nanopore ionic current blockade signatures. This approach coupled with polymerase attached to the nanopores in an array format should yield a single-molecule electronic Nano-SBS platform.

In previous work, the Center of Genome Technology & Biomolecular Engineering at Columbia University, led by Professor Ju and Dr. Nicholas J. Turro (William P. Schweitzer Professor of Chemistry), developed a four-color DNA sequencing by synthesis (SBS) platform using cleavable fluorescent nucleotide reversible terminators (NRT), which is licensed to Intelligent Bio-Systems, Inc., a QIAGEN company. SBS with cleavable fluorescent NRTs is the dominant approach
'/>"/>

Contact: Beth Kwon
beth.kwon@columbia.edu
212-854-6581
Columbia University
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Columbia Engineering and Penn researchers increase speed of single-molecule measurements
2. Forensic science used to determine whos who in pre-Columbian Peru
3. Scientists reconstruct pre-Columbian human effects on the Amazon Basin
4. Winemaking goes high-tech at the University of British Columbia
5. Columbia awarded 1 of first Provocative Questions grants from NCI
6. Study by UC Santa Barbara researchers suggests that bacteria communicate by touch
7. UC Santa Barbara researchers discover genetic link between visual pathways of hydras and humans
8. Researchers attempt to solve problems of antibiotic resistance and bee deaths in one
9. UNH researchers find African farmers need better climate change data to improve farming practices
10. Ottawa researchers to lead world-first clinical trial of stem cell therapy for septic shock
11. Researchers uncover molecular pathway through which common yeast becomes fungal pathogen
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:12/11/2014)... -- That blood pressure plays a role in human health has ... term for high blood pressure – was first described as ... that,s used in measuring blood pressure was invented in 1896. ... hypertension, its triggers and its effects. In fact, recent findings ... best ways to treat it. For example, a ...
(Date:12/10/2014)... 2014 You,ve been here before: you desperately need ... your password, site key or the answer to your secret ... teacher? Today, Hoyos Labs , a ... will finally put an end to the frustration that comes ... . 1U leverages a user,s smartphone to acquire his or ...
(Date:12/10/2014)... N.C. , Dec. 9, 2014  Wake Forest Baptist ... education building for its School of Medicine. Funding for ... larger capital campaign that will be publicly launched next ... be located in the former 60 series R.J. Reynolds ... Innovation Quarter. Construction will begin immediately with plans to ...
Breaking Biology News(10 mins):Research points to need for new approaches to treatment of high blood pressure 2Research points to need for new approaches to treatment of high blood pressure 3Research points to need for new approaches to treatment of high blood pressure 4The Password is Finally Dead: Launch of 1U Mobile App Eliminates Need for All Usernames and Passwords 2The Password is Finally Dead: Launch of 1U Mobile App Eliminates Need for All Usernames and Passwords 3Wake Forest Baptist to Build New Medical Education Facility In Wake Forest Innovation Quarter 2Wake Forest Baptist to Build New Medical Education Facility In Wake Forest Innovation Quarter 3Wake Forest Baptist to Build New Medical Education Facility In Wake Forest Innovation Quarter 4
... in Panama to use genetic barcoding, scientists at the ... 20 sand fly species from Barro Colorado Island. Two ... causes cutaneous leishmaniasis: persistent, itchy skin lesions. Three species ... that could contribute to a strategy to control the ...
... April 6, 2010 -- Spectacularly increased yields and improved ... researchers at the Robert H. Smith Faculty of Agriculture, ... Cold Spring Harbor Laboratory (CSHL), New York. The ... gene, which controls when plants make flowers and that ...
... scientists from around the world are involved in a ... research on the increasingly fragile relationship between humans and ... research as a priority and has selected MSU to ... on Coupled Human and Natural Systems, or CHANS-Net. The ...
Cached Biology News:Sand fly barcoding in Panama reveals Leishmania strain and its potential control 2Genetic key discovered to dramatically increase yields and improve taste of hybrid tomato plants 2Genetic key discovered to dramatically increase yields and improve taste of hybrid tomato plants 3MSU leads global effort to study link between people, planet 2
(Date:12/22/2014)... Dec. 22, 2014  ( www.competitivehealth.com ) — Competitive ... medical bill review and advocacy service, has signed an ... WellCard Savings discount health services marketplace. 63% ... more than they expected to pay. As part of ... costs, WellCard Savings is pleased to offer medical bill ...
(Date:12/22/2014)... 22, 2014 Fertility Associates of Memphis ... Bank USA , a network of more than 20 ... partnership, patients have fast access to a national pool ... IVF . Fertility Associates of Memphis is the only ... treatment option. , Dr. William Kutteh , director ...
(Date:12/19/2014)... BioPlus Specialty Pharmacy (BioPlus), one ... of Nick Maroulis, Pharm.D. to the newly created position ... In this position, Dr. Maroulis will continue to manage ... of our multi-site pharmacies as the company expands. Dr. ... that time he has served in many different capacities ...
(Date:12/19/2014)... Dec. 19, 2014 Bina Technologies, Inc. (Bina, ... that they have been acquired by Roche (SIX: RO, ... privately held company that provides a big data platform ... (NGS) data for the academic and translational research markets. ... and will continue to focus on development of their ...
Breaking Biology Technology:WellCard Savings Announces CoPatient Medical Bill Review Service for Members 2Fertility Associates of Memphis Announces Partnership with Donor Egg Bank USA 2Fertility Associates of Memphis Announces Partnership with Donor Egg Bank USA 3BioPlus Specialty Pharmacy Promotes Dr. Nick Maroulis to Vice President of Specialty Pharmacy Services 2Bina Technologies is acquired by Roche. 2Bina Technologies is acquired by Roche. 3
... , Three-of-Four Patients Surveyed Experience Daily Pain From ... the European League Against Rheumatism Annual Congress of ... Results from the Rheumatoid Arthritis: Insights, Strategies ... rheumatoid arthritis (RA) patients throughout Europe and Canada ...
... SPRINGFIELD, Va., June 9 Methuselah Foundation has identified ... of bio-printing. The US Department of Health and ... be the standard of care for replacing all tissue/organ ... Methuselah Foundation, is applying its breakthrough organ printing technologies ...
... and Patient Reported Outcome Strategies , , PITTSBURGH, ... leader in electronic patient reported outcomes (ePRO) solutions and ... ePROficiency(TM) Web seminar series on Patient Reported Outcomes (PRO). ... a continuation of invivodata,s educational initiative which focuses on ...
Cached Biology Technology:New Survey Reveals That More Than Half of Rheumatoid Arthritis Patients in Europe and Canada Feel Their Disease Controls Their Lives 2New Survey Reveals That More Than Half of Rheumatoid Arthritis Patients in Europe and Canada Feel Their Disease Controls Their Lives 3New Survey Reveals That More Than Half of Rheumatoid Arthritis Patients in Europe and Canada Feel Their Disease Controls Their Lives 4New Survey Reveals That More Than Half of Rheumatoid Arthritis Patients in Europe and Canada Feel Their Disease Controls Their Lives 5New Survey Reveals That More Than Half of Rheumatoid Arthritis Patients in Europe and Canada Feel Their Disease Controls Their Lives 6Printing New Organs: Foundation Funds Medical Technology That Replicates Human Tissue 2invivodata Announces ePROficiency 2009 Web Seminar Series 2