Navigation Links
Columbia engineer observes surprising behavior of cells during blood-vessel formation
Date:3/7/2011

Biologists tend to look at cells in bulk, observing them as a group and taking the average behavior as the norm the assumption is that genetically identical cells all behave the same way. In a paper to be published in the online Early Edition of Proceedings of the National Academy of Sciences the week of March 7, 2011, Sam Sia, assistant professor of biomedical engineering at Columbia Engineering, presents the results of his four-year tissue-engineering study that show a surprising range of variation in how individual cells behave during formation of a blood vessel. Sia and his team used a new method to painstakingly observe and track individual behaviors, characterizing, for the first time, what happens when human endothelial cells move from an initial dispersed state to the formation of capillary-like structures.

"We were really surprised by this behavior," says Sia, who was named one of the world's top young innovators for 2010 by MIT's Technology Review for his work in biotechnology and medicine. "In contrast to the population-averaged behavior that most studies report, most individual cells followed distinct patterns of cell-shape changes that were not reflected in the bulk average."

This is one of the first explicit studies to look at the variations between cells during tissue formation, and overturns the assumption that genetically identical cells behave in generally similar ways. Using a systematic approach to quantifying the changes in cell shape and movement for every single endothelial cell over time, the Columbia Engineering team found unexpected hidden patterns in behavior. In addition to discovering that most cells behave differently from the average, the team also observed that groups of cells behaved in similar fashions, and that some of these clusters of behavior resulted in distinct structural roles in the final blood-vessel network.

The origins of the variations in behavior are not known right now. Sia notes that "one possibility is simply random noise or naturally occurring fluctuations, which have been shown by other researchers to be important in producing biologically significant variations in gene expression and other subcellular processes. It's also possible there are subtle local variations in the extracellular environment that we're not aware of yet."

Sia says an application of this work is to exploit his technique to identify new drugs that modify angiogenesis. "A lot of drugs that either help or hinder blood-vessel formation have unknown mechanisms. This technique can potentially unravel some of those mechanisms, and help identify compounds that modulate specific aspects of how blood vessels form." In addition, knowledge of how individual cells behave will help in high-precision tissue engineering, an ongoing field of research in Sia's lab. "Knowledge of how individual cells or groups of cells behave enhances our understanding of how native tissues self-organize," he says. "This could ultimately enable more precise approaches for engineering complex multicellular tissues."

Sia was also named in 2010 by NASA as one of the ten innovators in human health and sustainability. In 2008, he received a CAREER award from the National Science Foundation that included a $400,000 grant to support his other research specialty in three-dimensional tissue engineering. A recipient of the Walter H. Coulter Early Career Award in 2008, Sia participated in the National Academy of Engineering's U.S. Frontiers of Engineering symposium for the nation's brightest young engineers in 2007.

His research is focused on developing new high-resolution tools to control the extracellular environments around cells, in order to study how they interact to form human tissues and organs. His lab uses techniques from a number of different fields, including biochemistry, molecular biology, microfabrication, microfluidics, materials chemistry, and cell and tissue biology.


'/>"/>

Contact: Holly Evarts
holly@engineering.columbia.edu
212-854-3206
Columbia University
Source:Eurekalert

Related biology news :

1. Columbia University uses technological innovation to study bone structure
2. Columbia University Medical Center announces 2010 Katz Prizes in cardiovascular research
3. Columbia engineer part of team to receive $6.2 million DOD grant for blast research
4. Columbia University Medical Center announces 2009 Katz Prizes in Cardiovascular Research
5. Columbia University scientist devises new way to more rapidly generate bone tissue
6. Salmon smolt survival similar in Columbia and Fraser rivers
7. Similar survival rates for Pacific salmon in Fraser, Columbia Rivers raises new questions
8. Columbia to award 2008 Horwitz Prize to Arthur Horwich & Ulrich Hartl for cellular protein folding
9. Civil engineers to honor NJITs Priscilla Nelson for research
10. Engineering students win award from National Clean Energy Contest
11. UCLA Engineering advance with new nanomaterials good news for next-generation electronic devices
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/29/2016)... 29, 2016 LegacyXChange, Inc. (OTC: ... and SelectaDNA/CSI Protect are pleased to announce our successful ... a variety of writing instruments, ensuring athletes signatures against ... collectibles from athletes on LegacyXChange will be assured of ... DNA. Bill Bollander , CEO states, ...
(Date:3/22/2016)... , March 22, 2016 ... research report "Electronic Sensors Market for Consumer Industry by ... & Others), Application (Communication & IT, Entertainment, ... - Global Forecast to 2022", published by ... is expected to reach USD 26.76 Billion ...
(Date:3/21/2016)... -- Unique technology combines v ...   Xura, Inc. (NASDAQ: ... communications services, today announced it is working alongside SpeechPro ... particularly those in the Financial Services Sector, the ability ... a mobile app, alongside, and in combination with, traditional ...
Breaking Biology News(10 mins):
(Date:6/24/2016)... TOKYO , June 24, 2016  Regular discussions on ... to take place between the two entities said Poloz. ... in Ottawa , he pointed to the ... and the federal government. ... Poloz said, "Both institutions have common economic goals, why not ...
(Date:6/24/2016)... (PRWEB) , ... June 24, 2016 , ... Researchers at ... most commonly-identified miRNAs in people with peritoneal or pleural mesothelioma. Their findings are the ... read it now. , Diagnostic biomarkers are signposts in the blood, lung fluid ...
(Date:6/23/2016)... , June 23, 2016   Boston ... of novel compounds designed to target cancer stemness ... has been granted Orphan Drug Designation from the ... treatment of gastric cancer, including gastroesophageal junction (GEJ) ... inhibitor designed to inhibit cancer stemness pathways by ...
(Date:6/23/2016)... , June 23, 2016 Houston Methodist ... the Cy-Fair Sports Association to serve as their ... agreement, Houston Methodist Willowbrook will provide sponsorship support, ... connectivity with association coaches, volunteers, athletes and families. ... the Cy-Fair Sports Association and to bring Houston ...
Breaking Biology Technology: