Shepard's group has been interested in single-molecule measurements for several years looking at a variety of novel transduction platforms. They began working with nanopore sensors after Marija Drndic, a professor of physics at the University of Pennsylvania, gave a seminar at Columbia Engineering in 2009. "We saw that nearly everybody else measures nanopores using classical electrophysiology amplifiers, which are mostly optimized for slower measurements," notes Shepard. "So we designed our own integrated circuit instead."
Rosenstein designed the new electronics and did much of the lab work. Drndic's group at the University of Pennsylvania fabricated the nanopores that the team then measured in their new system.
"While most groups are trying to slow down DNA, our approach is to build faster electronics," says Drndic. "We combined the most sensitive electronics with the most sensitive solid-state nanopores."
"It's very exciting to be able to make purely electronic measurements of single molecules," says Rosenstein. "The setup for nanopore measurements is very simple and portable. It doesn't require a complicated microscope or high powered instruments; it just requires attention to detail. You can easily imagine nanopore technology having a major impact on DNA sequencing and other med
|Contact: Holly Evarts|