Navigation Links
Cold Spring Harbor Protocols highlights gene silencing, cancer cell biology methods
Date:8/4/2008

COLD SPRING HARBOR, N.Y. Combining the specificity of small interfering RNA (siRNA) silencing with the versatility of lentiviral vectors gives researchers a powerful tool for the investigation of gene function both in vivo and in vitro. This month's issue of Cold Spring Harbor Protocols (www.cshprotocols.org/TOCs/toc8_08.dtl) features a pair of protocols from Inder Verma (http://www.salk.edu/faculty/faculty_details.php?id=54) and colleagues at the Salk Institute describing this method for achieving long-term down-regulation of specific target genes in a wide range of cell types. "Design and Cloning of an shRNA into a Lentiviral Vector" is freely accessible on the website for Cold Spring Harbor Protocols (http://www.cshprotocols.org/cgi/content/full/2008/9/pdb.prot5009)

The second featured protocol for August, "In Vitro Assays for the Extracellular Matrix Protein-Regulated Extravasation Process," provides a method for studying the ways that cancerous cells pass through blood vessel walls, a critical step in metastasis. Written by Xiao-Fan Wang (http://pharmacology.mc.duke.edu/faculty/wang.htm) and colleagues from Duke University, this protocol includes two methods for assaying the role of extracellular matrix proteins in cancer cell extravasation. This article is freely accessible on the website for Cold Spring Harbor Protocols (http://www.cshprotocols.org/cgi/content/full/2008/9/pdb.prot5034).


'/>"/>

Contact: David Crotty
crotty@cshl.edu
516-422-4007
Cold Spring Harbor Laboratory
Source:Eurekalert

Page: 1

Related biology news :

1. Springer increases publishing activities in South Korea
2. Springer will publish Journal of Coastal Conservation
3. Springer book wins Quality of Communication Award
4. Species still have more viable offspring if they can choose their best mate
5. From brains to behavior: Cold Spring Harbor Protocols features methods for neuroscience research
6. Estuarine Research Federation chooses Springer as publishing partner
7. Springer and the New York Botanical Garden Press join forces to publish botanical journals
8. Cold Spring Harbor Protocols highlights a method that captures cell growth and activity
9. Hellish hot springs yield greenhouse gas-eating bug
10. Springer founds new journal Air Quality, Atmosphere and Health
11. Moms obesity during conception phase may set the stage for offsprings obesity risk
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/6/2017)... LONDON , April 6, 2017 ... Control, RFID, ANPR, Document Readers, by End-Use (Transportation & ... Energy Facility, Oil, Gas & Fossil Generation Facility, Nuclear ... Healthcare, Educational, Other) Are you looking for ... Authentication sector? ...
(Date:4/4/2017)... --  EyeLock LLC , a leader of iris-based identity ... and Trademark Office (USPTO) has issued U.S. Patent No. ... iris image with a face image acquired in sequence ... th issued patent. "The issuance ... multi-modal biometric capabilities that have recently come to market ...
(Date:3/30/2017)... YORK , March 30, 2017 Trends, ... type (physiological and behavioral), by technology (fingerprint, AFIS, iris ... voice recognition, and others), by end use industry (government ... and immigration, financial and banking, and others), and by ... Europe , Asia Pacific , ...
Breaking Biology News(10 mins):
(Date:10/11/2017)... ... October 11, 2017 , ... At ... Purple announced Dr. Suneel I. Sheikh, the co-founder, CEO and chief research scientist ... has been selected for membership in ARCS Alumni Hall of Fame . ...
(Date:10/11/2017)... INDIANAPOLIS , Oct. 11, 2017  VMS BioMarketing, a ... of a nationwide oncology Clinical Nurse Educator (CNE) network, which ... growing need for communication among health care professionals to enhance ... physicians, nurses, office staff, and other health care professionals to ... for breast cancer. ...
(Date:10/10/2017)... ... 2017 , ... San Diego-based team building and cooking events company, Lajollacooks4u, has ... The bold new look is part of a transformation to increase awareness, appeal to ... period. , It will also expand its service offering from its signature gourmet cooking ...
(Date:10/10/2017)... 2017 SomaGenics announced the receipt of a ... RealSeq®-SC (Single Cell), expected to be the first commercially ... microRNAs) from single cells using NGS methods. The NIH,s ... accelerate development of approaches to analyze the heterogeneity of ... techniques for measuring levels of mRNAs in individual cells ...
Breaking Biology Technology: