Navigation Links
Cold Spring Harbor Protocols highlights gene silencing, cancer cell biology methods
Date:8/4/2008

COLD SPRING HARBOR, N.Y. Combining the specificity of small interfering RNA (siRNA) silencing with the versatility of lentiviral vectors gives researchers a powerful tool for the investigation of gene function both in vivo and in vitro. This month's issue of Cold Spring Harbor Protocols (www.cshprotocols.org/TOCs/toc8_08.dtl) features a pair of protocols from Inder Verma (http://www.salk.edu/faculty/faculty_details.php?id=54) and colleagues at the Salk Institute describing this method for achieving long-term down-regulation of specific target genes in a wide range of cell types. "Design and Cloning of an shRNA into a Lentiviral Vector" is freely accessible on the website for Cold Spring Harbor Protocols (http://www.cshprotocols.org/cgi/content/full/2008/9/pdb.prot5009)

The second featured protocol for August, "In Vitro Assays for the Extracellular Matrix Protein-Regulated Extravasation Process," provides a method for studying the ways that cancerous cells pass through blood vessel walls, a critical step in metastasis. Written by Xiao-Fan Wang (http://pharmacology.mc.duke.edu/faculty/wang.htm) and colleagues from Duke University, this protocol includes two methods for assaying the role of extracellular matrix proteins in cancer cell extravasation. This article is freely accessible on the website for Cold Spring Harbor Protocols (http://www.cshprotocols.org/cgi/content/full/2008/9/pdb.prot5034).


'/>"/>

Contact: David Crotty
crotty@cshl.edu
516-422-4007
Cold Spring Harbor Laboratory
Source:Eurekalert

Page: 1

Related biology news :

1. Springer increases publishing activities in South Korea
2. Springer will publish Journal of Coastal Conservation
3. Springer book wins Quality of Communication Award
4. Species still have more viable offspring if they can choose their best mate
5. From brains to behavior: Cold Spring Harbor Protocols features methods for neuroscience research
6. Estuarine Research Federation chooses Springer as publishing partner
7. Springer and the New York Botanical Garden Press join forces to publish botanical journals
8. Cold Spring Harbor Protocols highlights a method that captures cell growth and activity
9. Hellish hot springs yield greenhouse gas-eating bug
10. Springer founds new journal Air Quality, Atmosphere and Health
11. Moms obesity during conception phase may set the stage for offsprings obesity risk
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/17/2016)... LONDON , March 17, 2016 ... market intelligence, forecasts the global biometrics market will ... an impressive 118% increase from 2015. Consumer electronics, ... with embedded fingerprint sensors anticipated to reach two ... Dimitrios Pavlakis , Research Analyst ...
(Date:3/11/2016)... 11, 2016 http://www.apimages.com ) - --> ... is available at AP Images ( http://www.apimages.com ) - ... used to produce the new refugee identity cards. DERMALOG will be ... CeBIT in Hanover next week.   --> ... be used to produce the new refugee identity cards. DERMALOG will ...
(Date:3/9/2016)... HAMBURG, Germany , March 9, 2016 ... African country,s government identified that more than 23,000 public ... name or had been receiving their salary unlawfully.    ... West African country,s government identified that more than 23,000 ... recorded name or had been receiving their salary unlawfully. ...
Breaking Biology News(10 mins):
(Date:5/3/2016)... ... May 03, 2016 , ... ... CEO 2016 on May 31st and June 1st at The Four Seasons Hotel ... executives in the life sciences, offering exclusive access to key decision makers who ...
(Date:5/3/2016)... , May 3, 2016  Dr. Thomas ... surgeon in The Woodlands, Texas , ... 24 percent of treated fat cells in just 25-minutes, ... Close to 90 percent of Americans report feeling bothered ... Nonsurgical fat reduction procedures are a growing industry. This ...
(Date:5/2/2016)... ... May 02, 2016 , ... StarNet Communications Corp, ( http://www.starnet.com/ ... addition of three Secure Remote Desktop modules to its flagship X-Win32 PC X server. ... and Unix servers to the user’s PC over encrypted SSH. , Traditionally, users of ...
(Date:4/29/2016)... , April 29, 2016 ... Transparency Market Research "Separation Systems for Commercial Biotechnology ... Trends, and Forecast 2015 - 2023", the separation ... US$ 10,665.5 Mn in 2014 and is projected ... 2015 to 2023 to reach US$ 19,227.8 Mn ...
Breaking Biology Technology: