Navigation Links
Cold Spring Harbor Protocols highlights a method that captures cell growth and activity
Date:11/1/2007

COLD SPRING HARBOR, N.Y. (Thurs., Nov. 1, 2007) This months issue of Cold Spring Harbor Protocols (www.cshprotocols.org) features a cutting-edge method that provides a snapshot of growth and activity patterns in mixed populations of cells. The protocol is freely accessible online (http://www.cshprotocols.org/cgi/content/full/2007/22/pdb.prot4895).

Written by Ingrid Schmid, a scientist at UCLA (http://cyto.mednet.ucla.edu/), the protocol involves taking a population of cells and labeling their nucleic acids (DNA and RNA) and cell-surface proteins with specific dyes. Then, using a technique called flow cytometry, scientists can determine the DNA and RNA content of the cells. This allows them to identify the cell-cycle stage of each cell, which provides insight into patterns of cell growth and activity in the population. For example, they can identify which sub-populations of cells are actively dividing and which are not.

Schmid and her colleagues have used the procedure to characterize specific sub-populations of cells in human blood that are involved in the immune response.

This months issue of Cold Spring Harbor Protocols also includes the related classic technique for the quantification of DNA and RNA in solutions. This essential and routine procedure is used in virtually every laboratory. This article is also freely available online (http://www.cshprotocols.org/cgi/content/full/2007/22/pdb.ip47).


'/>"/>

Contact: Maria Smit
smit@cshl.edu
516-422-4127
Cold Spring Harbor Laboratory
Source:Eurekalert

Page: 1

Related biology news :

1. Report that delayed motherhood decreases life expectancy of mouse offspring
2. Nano springs eternal; Protozoan engine posts nano records
3. Hot-spring bacteria flip a metabolic switch
4. Spring migration of pink-footed geese under threat
5. Protein nanosprings most resilient found in nature
6. Males with elevated levels of testosterone lead shorter lives but have more success siring offspring
7. Genetic snooze button governs timing of spring flowers
8. From hot springs to rice farms, scientists reveal new insights into the secret lives of archaea
9. Empty nester parent birds use recruitment calls to extend offspring care
10. The price of vanity: Mating with showy males may reduce offsprings ability to fight off pathogens
11. Lighting up life: Cold Spring Harbor Protocols presents tips for creating glowing plants
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/30/2017)... , March 30, 2017  On April 6-7, 2017, ... the Genome hackathon at Microsoft,s headquarters in ... competition will focus on developing health and wellness apps ... Hack the Genome is the first hackathon ... The world,s largest companies in the genomics, tech and ...
(Date:3/29/2017)... 29, 2017  higi, the health IT company that ... North America , today announced a Series B ... of EveryMove. The new investment and acquisition accelerates higi,s ... to transform population health activities through the collection and ... higi collects and secures data today on behalf ...
(Date:3/24/2017)... 24, 2017 The Controller General of Immigration from ... Abdulla Algeen have received the prestigious international IAIR Award for the ... Continue Reading ... ... Controller Abdulla Algeen (small picture on the right) have received the IAIR ...
Breaking Biology News(10 mins):
(Date:10/11/2017)... ... October 11, 2017 , ... ... has granted orphan drug designation to SBT-100, its novel anti-STAT3 (Signal Transducer and ... of osteosarcoma. SBT-100 is able to cross the cell membrane and bind intracellular ...
(Date:10/10/2017)... , ... October 10, 2017 ... ... cancer-focused pharmaceutical company advancing targeted antibody-drug conjugate (ADC) therapeutics, today confirmed licensing ... HPLN (Hybrid Polymerized Liposomal Nanoparticle), a technology developed in collaboration with Children’s ...
(Date:10/10/2017)... (PRWEB) , ... October 10, 2017 , ... Dr. Bob ... at his local San Diego Rotary Club. The event entitled “Stem ... CA and had 300+ attendees. Dr. Harman, DVM, MPVM was joined by two ...
(Date:10/10/2017)... Oct. 10, 2017 SomaGenics announced the receipt ... to develop RealSeq®-SC (Single Cell), expected to be the ... RNAs (including microRNAs) from single cells using NGS methods. ... need to accelerate development of approaches to analyze the ... "New techniques for measuring levels of mRNAs in ...
Breaking Biology Technology: