Navigation Links
Cold Spring Harbor Protocols highlights a method that captures cell growth and activity
Date:11/1/2007

COLD SPRING HARBOR, N.Y. (Thurs., Nov. 1, 2007) This months issue of Cold Spring Harbor Protocols (www.cshprotocols.org) features a cutting-edge method that provides a snapshot of growth and activity patterns in mixed populations of cells. The protocol is freely accessible online (http://www.cshprotocols.org/cgi/content/full/2007/22/pdb.prot4895).

Written by Ingrid Schmid, a scientist at UCLA (http://cyto.mednet.ucla.edu/), the protocol involves taking a population of cells and labeling their nucleic acids (DNA and RNA) and cell-surface proteins with specific dyes. Then, using a technique called flow cytometry, scientists can determine the DNA and RNA content of the cells. This allows them to identify the cell-cycle stage of each cell, which provides insight into patterns of cell growth and activity in the population. For example, they can identify which sub-populations of cells are actively dividing and which are not.

Schmid and her colleagues have used the procedure to characterize specific sub-populations of cells in human blood that are involved in the immune response.

This months issue of Cold Spring Harbor Protocols also includes the related classic technique for the quantification of DNA and RNA in solutions. This essential and routine procedure is used in virtually every laboratory. This article is also freely available online (http://www.cshprotocols.org/cgi/content/full/2007/22/pdb.ip47).


'/>"/>

Contact: Maria Smit
smit@cshl.edu
516-422-4127
Cold Spring Harbor Laboratory
Source:Eurekalert

Page: 1

Related biology news :

1. Report that delayed motherhood decreases life expectancy of mouse offspring
2. Nano springs eternal; Protozoan engine posts nano records
3. Hot-spring bacteria flip a metabolic switch
4. Spring migration of pink-footed geese under threat
5. Protein nanosprings most resilient found in nature
6. Males with elevated levels of testosterone lead shorter lives but have more success siring offspring
7. Genetic snooze button governs timing of spring flowers
8. From hot springs to rice farms, scientists reveal new insights into the secret lives of archaea
9. Empty nester parent birds use recruitment calls to extend offspring care
10. The price of vanity: Mating with showy males may reduce offsprings ability to fight off pathogens
11. Lighting up life: Cold Spring Harbor Protocols presents tips for creating glowing plants
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:1/6/2017)... , Jan. 6, 2017  Privately-held CalciMedica, Inc., ... in healthy volunteers of a novel calcium release-activated ... acute pancreatitis. Acute pancreatitis, sudden ... mild disorder, but can be very serious.  In severe ... sepsis, where extended hospital stays, time in the ...
(Date:1/4/2017)... 2017  For the thousands of attendees at this year,s International Consumer ... health and biometric measurement devices and services, will be featuring its new ... in A&D Medical,s special CES Exhibit Suite , the new upper ... the company,s WellnessConnected product platform.  ... ...
(Date:12/22/2016)... MOUNTAIN VIEW, Calif. , Dec. 20, 2016  As ... all levels, 23andMe, the leading personal genetics company, recently released ... Only Me . The book focuses on the topics ... the Next Generation Science Standards (NGSS) taught in elementary school ... the second in a series by illustrator Ariana Killoran ...
Breaking Biology News(10 mins):
(Date:1/18/2017)... , Jan. 18, 2017  HUYA Bioscience International, (HUYA), ... China,s pharmaceutical innovations, announced today a ... Innovation and Investment Company (referred to as CAS Innovation). ... innovations discovered by leading scientists at CAS to meet ... HUYA is the first company to have recognized ...
(Date:1/17/2017)... West Point, IA (PRWEB) , ... January 17, ... ... approval from China for Balance™ GT soybeans. The new Balance™ GT Soybean Performance ... tolerance to glyphosate and isoxaflutole, the active ingredient in the new Balance® Bean ...
(Date:1/17/2017)... Mass. , Jan. 17, 2017   Pulmatrix, ... stage biopharmaceutical company developing innovative inhaled therapies to address ... for treating fungal infections in the lungs of CF ... Disease Product" (QIDP) by the U.S. Food & Drug ... which is designed to speed the development of novel ...
(Date:1/17/2017)... UK (PRWEB) , ... January ... ... presented by surgeons at the 2016 annual meeting of the North American ... clinical outcomes, for patients in the majority of cases, when PEEK-OPTIMA™ HA ...
Breaking Biology Technology: