Navigation Links
Cold Spring Harbor Protocols highlights a method that captures cell growth and activity
Date:11/1/2007

COLD SPRING HARBOR, N.Y. (Thurs., Nov. 1, 2007) This months issue of Cold Spring Harbor Protocols (www.cshprotocols.org) features a cutting-edge method that provides a snapshot of growth and activity patterns in mixed populations of cells. The protocol is freely accessible online (http://www.cshprotocols.org/cgi/content/full/2007/22/pdb.prot4895).

Written by Ingrid Schmid, a scientist at UCLA (http://cyto.mednet.ucla.edu/), the protocol involves taking a population of cells and labeling their nucleic acids (DNA and RNA) and cell-surface proteins with specific dyes. Then, using a technique called flow cytometry, scientists can determine the DNA and RNA content of the cells. This allows them to identify the cell-cycle stage of each cell, which provides insight into patterns of cell growth and activity in the population. For example, they can identify which sub-populations of cells are actively dividing and which are not.

Schmid and her colleagues have used the procedure to characterize specific sub-populations of cells in human blood that are involved in the immune response.

This months issue of Cold Spring Harbor Protocols also includes the related classic technique for the quantification of DNA and RNA in solutions. This essential and routine procedure is used in virtually every laboratory. This article is also freely available online (http://www.cshprotocols.org/cgi/content/full/2007/22/pdb.ip47).


'/>"/>

Contact: Maria Smit
smit@cshl.edu
516-422-4127
Cold Spring Harbor Laboratory
Source:Eurekalert

Page: 1

Related biology news :

1. Report that delayed motherhood decreases life expectancy of mouse offspring
2. Nano springs eternal; Protozoan engine posts nano records
3. Hot-spring bacteria flip a metabolic switch
4. Spring migration of pink-footed geese under threat
5. Protein nanosprings most resilient found in nature
6. Males with elevated levels of testosterone lead shorter lives but have more success siring offspring
7. Genetic snooze button governs timing of spring flowers
8. From hot springs to rice farms, scientists reveal new insights into the secret lives of archaea
9. Empty nester parent birds use recruitment calls to extend offspring care
10. The price of vanity: Mating with showy males may reduce offsprings ability to fight off pathogens
11. Lighting up life: Cold Spring Harbor Protocols presents tips for creating glowing plants
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:2/3/2017)... 2017 A new independent identity strategy consultancy ... (IdSP) . Designed to fill a critical niche in ... founding partners Mark Crego and Janice ... in identity expertise that span federal governments, the 9/11 ... Crego-Kephart combined expertise has a common theme born from ...
(Date:2/2/2017)... 1, 2017  Central to its deep commitment ... worldwide, The Japan Prize Foundation today announced the ... pushed the envelope in their respective fields of ... scientists are being recognized with the 2017 Japan ... only contribute to the advancement of science and ...
(Date:1/30/2017)... 2017   Invitae Corporation (NYSE: ... companies, today announced that it will report its fourth ... guidance on Monday, February 13, 2017, and Invitae,s management ... 4:45 p.m. Eastern / 1:45 p.m. Pacific. ... review financial results, guidance, and recent developments and will ...
Breaking Biology News(10 mins):
(Date:2/23/2017)... ... February 23, 2017 , ... ... evaluation of multiple immunoassay-based threat detection technologies by researchers from the Pacific ... biosensor threat detection technology was found to have the best level of ...
(Date:2/23/2017)... ... ... Today, researchers can fast-track sample collection and analysis for ... or SNPs of interest) using one, easy-to-collect saliva sample. With the addition of ... and other relevant biomarkers can be extensively studied through a non-invasive sample type. ...
(Date:2/23/2017)... , Feb. 23, 2017  Capricor Therapeutics, Inc. (NASDAQ: CAPR), ... medical conditions, today announced that Linda Marbán, Ph.D, president and ... investor conferences: Cowen and Company 37th ... am ET Boston, MA ... 9:00 am PT (12:00 pm ET) Dana Point, ...
(Date:2/23/2017)... FRANCISCO , Feb. 23, 2017   ViaCyte, ... Type 1, a not-for-profit advocacy and education group for ... grant from Beyond Type 1 to support ViaCyte,s efforts ... other insulin-requiring diabetes.  For more than ... cell replacement therapies with a focus on the treatment ...
Breaking Biology Technology: