Navigation Links
Cold Spring Harbor Protocols features stem cell differentiation, plant RNAi methods
Date:2/2/2009

COLD SPRING HARBOR, N.Y. (Mon., Feb. 2, 2009) -- By using OP9-DL1 cells as a support system, researchers can study the differentiation of embryonic stem cells into mature components of the immune system. This month's issue of Cold Spring Harbor Protocols (www.cshprotocols.org/TOCs/toc2_09.dtl) features a set of methods from Juan Carlos Ziga-Pflcker's laboratory at the University of Toronto (http://www.immunology.utoronto.ca/faculty/directory/zunigapflucker.htm) detailing "The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro." The article contains a series of protocols describing the establishment, maintenance, and storage of OP9 and OP9-DL1 cells; the co-culture of these cells with embryonic stem cells or hematopoietic stem cells from fetal liver or bone marrow; and the in vitro differentiation of the stem cells into lymphocytes. The OP9-DL1 system has been useful in addressing questions about the cellular and molecular regulation of T-lymphocyte lineage commitment, pre-T cell receptor signaling (beta-selection), functional characteristics of progenitor T cells, and maturation of functional CD8 T cells. The article is freely accessible on the website for Cold Spring Harbor Protocols (http://www.cshprotocols.org/cgi/content/full/2009/2/pdb.prot5156).

The second featured article for February comes from S.P. Dinesh-Kumar and colleagues at Yale University (http://plantfunctionalgenomics.yale.edu/). RNA interference (RNAi) has become an effective tool for the down-regulation of genes in plants. The most effective means of accomplishing this gene silencing is through the use of viral vectors, with the Tobacco Ratt
'/>"/>

Contact: David Crotty
crotty@cshl.edu
516-422-4007
Cold Spring Harbor Laboratory
Source:Eurekalert

Page: 1 2

Related biology news :

1. Springer author and advisory board member wins LOreal UNESCO Women in Science Award
2. Springer expands its portfolio in plant sciences with Phytoparasitica
3. Mothers pass on disease clues to offspring
4. A low dose of caffeine when pregnant may damage the heart of offspring for a lifetime
5. Springer and the Physiological Society of Japan sign publishing agreement
6. Springer looks ahead to the Future City
7. Springer partners with Botanical Society of Korea
8. Springer to publish Fisheries Science as of 2009
9. Eating eggs when pregnant affects breast cancer in offspring
10. Springer launches Food Security
11. International Journal of Social Robotics debuts at Springer
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/25/2015)... June 25, 2015  Imagine a tool specifically designed ... healthier, happier life. That,s exactly what USANA,s True ... The cutting-edge, portable health program provides a personalized approached ... help improve your lifestyle and nutrition. ... gala Wednesday night, USANA,s THA was honored with the ...
(Date:6/24/2015)... 2015 This report provides market analysis of ... years. It contains an analysis of the drivers, challenges, ... impact from the short, medium, and long term perspective ... market, and technology trends that currently, prevail in the ... authorities to efficiently manage the increasing marine traffic and ...
(Date:6/24/2015)... Biometry authentication provider KeyLemon today announced ... one face in. Logo - ... in, entering the expanding biometric authentication market in ... answer to the password problem. With advanced face ... KeyLemon, one face in allows users to securely ...
Breaking Biology News(10 mins):@USANAinc's Personalized Nutrition Program Wins Industry Innovation Award 2Automatic Identification System Market by Class, by Platform, by Application and by Geography - Global Forecasts & Analysis to 2014 - 2020 2KeyLemon Announces Partnership With Biometric Password Solution onefacein 2
... July 23, 2008. A fire is currently burning through a ... 2 years ago. Managers used data and analysis from the ... implementation of treatments near where the Cold Springs fire is ... of the Cascade Range in Washington, and covers about 15,000 ...
... manure into a domestic renewable fuel source could generate ... of North America,s entire consumption needs and lead to ... to US research published today, Thursday, 24 July, in ... The journal paper, ,Cow Power: The Energy and ...
... a study of parasites living in three estuaries on the ... that biomass of these parasites exceeds that of top predators, ... findings, which could have significant ecological and biomedical implications, appear ... Biomass is the amount of living matter that exists ...
Cached Biology News:Landscape study may offer solutions for fire managers 2Cow power could generate electricity for millions 2Parasites outweigh predators in Pacific Coast estuaries 2Parasites outweigh predators in Pacific Coast estuaries 3
(Date:6/30/2015)... 30, 2015  The University of California, San ... and UC San Diego Extension announced their third ... Aug.  12 – 14, 2015, at UC San ... provides an intense exposure to best practices and ... well-suited to biologists, process engineers and business executives ...
(Date:6/30/2015)... , June 30, 2015 /PRNewswire/ - RepliCel ... clinical stage regenerative medicine company focused on the ... first participant in its Phase 1/2 clinical trial ... chronic unilateral Achilles tendinosis, has been enrolled and ... intra-tendon ultrasound-guided injections. RCT-01 is comprised of non-bulbar ...
(Date:6/30/2015)... ... June 30, 2015 , ... Southern ... accepted the position of vice president of instruction at Chippewa Valley Technical College ... greatly appreciated the opportunity to lead SIUE during the past three years,” Furst-Bowe ...
(Date:6/30/2015)... Florida (PRWEB) , ... June 30, 2015 , ... ... cannabis digital media company, launched “Cannavoices,” an unprecedented digital video and broadcast ... largest but also the most dynamic collection of legal medical cannabis stories ever ...
Breaking Biology Technology:UC San Diego Extension to Offer Third Annual Microbial Fermentation Workshop 2RepliCel Life Sciences Enrolls First Participant in Phase 1/2 Clinical Trial of RCT-01 for Chronic Achilles Tendinosis 2RepliCel Life Sciences Enrolls First Participant in Phase 1/2 Clinical Trial of RCT-01 for Chronic Achilles Tendinosis 3RepliCel Life Sciences Enrolls First Participant in Phase 1/2 Clinical Trial of RCT-01 for Chronic Achilles Tendinosis 4SIUE’s Furst-Bowe Accepts VP of Instruction at CVTC 2SIUE’s Furst-Bowe Accepts VP of Instruction at CVTC 3Cannavoices Launches a Legal Medical Cannabis Documentary Initiative 2Cannavoices Launches a Legal Medical Cannabis Documentary Initiative 3
... and CYPRESS, Calif., Nov. 15, 2011 Quest Diagnostics ... provider of diagnostic testing, information and services, today announced ... new Simplexa Cytomegalovirus (CMV) molecular test kit on the ... in Europe. The test is designed to enable hospital ...
... strategic effort to refocus and strengthen its organization, the ... planning to make adjustments in the direction, roles, responsibilities ... made by Denise Brown, FRA Interim Executive Director, who ... board of directors with four of the area,s most ...
... LA JOLLA, Calif., Nov. 15, 2011 DermTech, ... disease detection, announced  it will present results from ... can be used with a high-throughput, low-cost qRT-PCR ... recapitulated on a qRT-PCR system that successfully discriminated ...
Cached Biology Technology:Quest Diagnostics CE Marks Simplexa™ Test for Cytomegalovirus for Sale in Europe 2Quest Diagnostics CE Marks Simplexa™ Test for Cytomegalovirus for Sale in Europe 3Fitzsimons Redevelopment Authority Rolls Out First Phase of Strategic Refocus With Appointment of Four Key Leaders in The Bioscience Industry to Its Board of Directors 2DermTech's Non-invasive Melanoma Detection Assay Poised to Enter Clinical Validation Study 2DermTech's Non-invasive Melanoma Detection Assay Poised to Enter Clinical Validation Study 3
... prep station, 100-120 V, is used to coat ... particles to create the source of the DNA ... the Helios gene gun. The station includes a ... unit, 12 foot Nalgene nitrogen regulator hose, two ...
Mouse polyclonal antibody to NR2F2 - nuclear receptor subfamily 2, group F, member 2...
The Discovery Series PDQuest 2-D analysis software is used for imaging, analyzing, and databasing two-dimensional electrophoretic gels. The 10-user network license entitles ten users to access data f...
Mouse monoclonal antibody raised against a partial recombinant NEK2. NCBI Entrez Gene ID = NEK2...
Biology Products: