Navigation Links
Cold Spring Harbor Protocols features stem cell differentiation, plant RNAi methods
Date:2/2/2009

COLD SPRING HARBOR, N.Y. (Mon., Feb. 2, 2009) -- By using OP9-DL1 cells as a support system, researchers can study the differentiation of embryonic stem cells into mature components of the immune system. This month's issue of Cold Spring Harbor Protocols (www.cshprotocols.org/TOCs/toc2_09.dtl) features a set of methods from Juan Carlos Ziga-Pflcker's laboratory at the University of Toronto (http://www.immunology.utoronto.ca/faculty/directory/zunigapflucker.htm) detailing "The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro." The article contains a series of protocols describing the establishment, maintenance, and storage of OP9 and OP9-DL1 cells; the co-culture of these cells with embryonic stem cells or hematopoietic stem cells from fetal liver or bone marrow; and the in vitro differentiation of the stem cells into lymphocytes. The OP9-DL1 system has been useful in addressing questions about the cellular and molecular regulation of T-lymphocyte lineage commitment, pre-T cell receptor signaling (beta-selection), functional characteristics of progenitor T cells, and maturation of functional CD8 T cells. The article is freely accessible on the website for Cold Spring Harbor Protocols (http://www.cshprotocols.org/cgi/content/full/2009/2/pdb.prot5156).

The second featured article for February comes from S.P. Dinesh-Kumar and colleagues at Yale University (http://plantfunctionalgenomics.yale.edu/). RNA interference (RNAi) has become an effective tool for the down-regulation of genes in plants. The most effective means of accomplishing this gene silencing is through the use of viral vectors, with the Tobacco Ratt
'/>"/>

Contact: David Crotty
crotty@cshl.edu
516-422-4007
Cold Spring Harbor Laboratory
Source:Eurekalert

Page: 1 2

Related biology news :

1. Springer author and advisory board member wins LOreal UNESCO Women in Science Award
2. Springer expands its portfolio in plant sciences with Phytoparasitica
3. Mothers pass on disease clues to offspring
4. A low dose of caffeine when pregnant may damage the heart of offspring for a lifetime
5. Springer and the Physiological Society of Japan sign publishing agreement
6. Springer looks ahead to the Future City
7. Springer partners with Botanical Society of Korea
8. Springer to publish Fisheries Science as of 2009
9. Eating eggs when pregnant affects breast cancer in offspring
10. Springer launches Food Security
11. International Journal of Social Robotics debuts at Springer
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:9/29/2014)... ramosum , coupled with a high-fat diet, may cause animals ... mBio , the online open-access journal of the American ... German Institute of Human Nutrition Potsdam-Rehbruecke in Nuthetal observed that ... gained weight when fed a high-fat diet. Mice that did ... when consuming a high-fat diet, and mice that had ...
(Date:9/29/2014)... WASHINGTON, DC September 29, 2014 -- Plants ... ,commensal, bacteria help the pants extract nutrients and ... preventing pathogens from contaminating fruits and vegetables. Now, ... commensal bacteria inside of seeds; thus ensuring that ... researchers, from the University of Notre Dame, presented ...
(Date:9/29/2014)... According to a NASA story from 2009, "human activities in ... fire emissions issue. Palm oil is increasingly grown for use ... fats in processed foods. It has become the most widely ... in recent years to surpass that of soybean oil. , ... has to be cleared to grow the crop, and the ...
Breaking Biology News(10 mins):Gut bacteria promote obesity in mice 2Plants prepackage beneficial microbes in their seeds 2
... (June 4, 2009) Weak enforcement combined with fishermen ... of fisheries regulations along the Northeastern United States coast. ... fisheries management measures put in place to protect and ... online this week in the journal Marine Policy ...
... 2009 | An international symposium to be ... of nuclear technologies to enhance animal nutrition and reproduction strategies ... transmitted to humans, such as swine and avian influenza. ... countries will present up-to-date findings for solving or alleviating factors ...
... function in your healthit protects you against viruses, ... In autoinflammatory diseases, however, the immune system goes ... from the National Institute of Arthritis and Musculoskeletal ... Institutes of Health, and other institutions have discovered ...
Cached Biology News:Study: Illegal fishing harming present and future New England groundfish fisheries 2FAO/IAEA Symposium to address animal production and health 2Scientists discover new genetic immune disorder in children 2Scientists discover new genetic immune disorder in children 3
(Date:10/2/2014)... and BRIDGEWATER, N.J. ... XON) , a leader in synthetic biology, and ... generic drug manufacturer by volume in the U.S., ... Channel Collaboration (ECC) for a target active pharmaceutical ... the goal of the collaboration is to develop ...
(Date:10/1/2014)... METTLER TOLEDO’s newly improved XS balance line includes ... as a dual range microbalance . As a ... pan delivers results up to twice as fast, even ... details mean users no longer need to suffer the ... front of the balance. Barbara Bienlein, medical specialist and ...
(Date:10/1/2014)... Greifensee, Switzerland (PRWEB) October 01, 2014 ... influence the quality of the final product. However, ... most regulated, regulatory frameworks such as Good Manufacturing ... is the responsibility of the manufacturer to interpret ... ensure consistent weighing performance quality. METTLER TOLEDO experts ...
(Date:10/1/2014)... As the world takes its first steps into the ... century would mark the beginning of a new era of ... fact that the world is experiencing a rapid rise in ... Almost half of these chronic conditions are due to lifestyle ... care and intervention. While the world is experiencing a rapid ...
Breaking Biology Technology:Intrexon and Amneal Expand Relationship with Second Product Collaboration 2Intrexon and Amneal Expand Relationship with Second Product Collaboration 3METTLER TOLEDO’s XS Balance Line Delivers Performance and Ergonomics To Safeguard Health in the Workplace 2METTLER TOLEDO’s XS Balance Line Delivers Performance and Ergonomics To Safeguard Health in the Workplace 3Comply with Weighing-Related Regulations in Pharma Manufacturing 2Fighting Mortality and Morbidity of Chronic Health Disorders during Open Access Week 2Fighting Mortality and Morbidity of Chronic Health Disorders during Open Access Week 3
... that it has received orders for the sale of ... far in its initial sales push,being led by new ... current efforts, shall announce such sales as and,when they ... an award-winning life sciences company focused on,the monitoring and ...
... Diagnostics Inc.,(TSX:SDI) today announced the market launch of ... for sepsis, in Germany through,A. Menarini Diagnostics Deutschland ... is the third most common cause of death ... of severe sepsis consumes,about one third of intensive ...
... /PRNewswire-FirstCall/ - AEterna Zentaris Inc.,(TSX: AEZ; Nasdaq: AEZS), a ... announced that its President and CEO,David J. Mazzo, Ph.D., ... Sciences Conference on Thursday, September 27, 2007 at 10:00 ... Hotel in New York City. A live webcast ...
Cached Biology Technology:Signalife Achieves $1.98 Million Sales Orders Thus Far 2Spectral partner launches EAA(TM) Rapid Sepsis Diagnostic in Germany 2
Lysozyme C (W-20)...
Request Info...
Abgene's ALTO-8 is the first automated decapper/capper for Abgene Twist-Lock tubes....
... X-Gal (5-bromo-4-chloro-3-indoyl-beta-D-galactopyranoside) is a ... identify recombinant plasmids. In ... and IPTG, lac ... blue, whereas recombinant colonies ...
Biology Products: