Navigation Links
Cold Spring Harbor Protocols features stem cell differentiation, plant RNAi methods

COLD SPRING HARBOR, N.Y. (Mon., Feb. 2, 2009) -- By using OP9-DL1 cells as a support system, researchers can study the differentiation of embryonic stem cells into mature components of the immune system. This month's issue of Cold Spring Harbor Protocols ( features a set of methods from Juan Carlos Ziga-Pflcker's laboratory at the University of Toronto ( detailing "The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro." The article contains a series of protocols describing the establishment, maintenance, and storage of OP9 and OP9-DL1 cells; the co-culture of these cells with embryonic stem cells or hematopoietic stem cells from fetal liver or bone marrow; and the in vitro differentiation of the stem cells into lymphocytes. The OP9-DL1 system has been useful in addressing questions about the cellular and molecular regulation of T-lymphocyte lineage commitment, pre-T cell receptor signaling (beta-selection), functional characteristics of progenitor T cells, and maturation of functional CD8 T cells. The article is freely accessible on the website for Cold Spring Harbor Protocols (

The second featured article for February comes from S.P. Dinesh-Kumar and colleagues at Yale University ( RNA interference (RNAi) has become an effective tool for the down-regulation of genes in plants. The most effective means of accomplishing this gene silencing is through the use of viral vectors, with the Tobacco Rattle Virus (TRV) providing the most robust results. "Virus-Induced Gene Silencing as a Tool for Delivery of dsRNA into Plants" outlines a simple procedure for introducing TRV-based vectors into commonly studied plants such as Arabidopsis and tomato. This method is freely accessible on the website for Cold Spring Harbor Protocols (


Contact: David Crotty
Cold Spring Harbor Laboratory

Related biology news :

1. Springer author and advisory board member wins LOreal UNESCO Women in Science Award
2. Springer expands its portfolio in plant sciences with Phytoparasitica
3. Mothers pass on disease clues to offspring
4. A low dose of caffeine when pregnant may damage the heart of offspring for a lifetime
5. Springer and the Physiological Society of Japan sign publishing agreement
6. Springer looks ahead to the Future City
7. Springer partners with Botanical Society of Korea
8. Springer to publish Fisheries Science as of 2009
9. Eating eggs when pregnant affects breast cancer in offspring
10. Springer launches Food Security
11. International Journal of Social Robotics debuts at Springer
Post Your Comments:
(Date:4/11/2017)... , April 11, 2017 No ... but researchers at the New York University Tandon ... of Engineering have found that partial similarities between ... systems used in mobile phones and other electronic ... The vulnerability lies in the fact ...
(Date:4/5/2017)... LONDON , April 4, 2017 KEY ... is anticipated to expand at a CAGR of 25.76% ... neurodegenerative diseases is the primary factor for the growth ... full report: MARKET INSIGHTS The ... of product, technology, application, and geography. The stem cell ...
(Date:3/30/2017)... , March 30, 2017 Trends, opportunities ... (physiological and behavioral), by technology (fingerprint, AFIS, iris recognition, ... recognition, and others), by end use industry (government and ... immigration, financial and banking, and others), and by region ... , Asia Pacific , and ...
Breaking Biology News(10 mins):
(Date:10/9/2017)... ... October 09, 2017 , ... ... journal, Epilepsia, Brain Sentinel’s SPEAC® System which uses the surface electromyography (sEMG) ... tonic-clonic seizures (GTCS) using surface electromyography (sEMG). The prospective multicenter phase III ...
(Date:10/7/2017)... ... October 06, 2017 , ... Phase ... metagenome deconvolution product, featuring the first commercially available Hi-C kit. Researchers can ... Hi-C metagenome deconvolution using their own facilities, supplementing the company’s full-service ProxiMeta ...
(Date:10/6/2017)... ... October 06, 2017 , ... The HealthTech Venture Network (HTVN) ... their fourth annual Conference where founders, investors, innovative practitioners and collaborators are invited ... competition showcasing early stage digital health and med tech companies. , This day-long ...
(Date:10/5/2017)... ... October 05, 2017 , ... LabRoots , the ... from around the world, is giving back to cancer research with a month-long promotion ... , Now through October 31, shoppers can use promo code PinkRibbon to get 10 ...
Breaking Biology Technology: