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Cold Spring Harbor Protocols features stem cell differentiation, plant RNAi methods
Date:2/2/2009

COLD SPRING HARBOR, N.Y. (Mon., Feb. 2, 2009) -- By using OP9-DL1 cells as a support system, researchers can study the differentiation of embryonic stem cells into mature components of the immune system. This month's issue of Cold Spring Harbor Protocols (www.cshprotocols.org/TOCs/toc2_09.dtl) features a set of methods from Juan Carlos Ziga-Pflcker's laboratory at the University of Toronto (http://www.immunology.utoronto.ca/faculty/directory/zunigapflucker.htm) detailing "The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro." The article contains a series of protocols describing the establishment, maintenance, and storage of OP9 and OP9-DL1 cells; the co-culture of these cells with embryonic stem cells or hematopoietic stem cells from fetal liver or bone marrow; and the in vitro differentiation of the stem cells into lymphocytes. The OP9-DL1 system has been useful in addressing questions about the cellular and molecular regulation of T-lymphocyte lineage commitment, pre-T cell receptor signaling (beta-selection), functional characteristics of progenitor T cells, and maturation of functional CD8 T cells. The article is freely accessible on the website for Cold Spring Harbor Protocols (http://www.cshprotocols.org/cgi/content/full/2009/2/pdb.prot5156).

The second featured article for February comes from S.P. Dinesh-Kumar and colleagues at Yale University (http://plantfunctionalgenomics.yale.edu/). RNA interference (RNAi) has become an effective tool for the down-regulation of genes in plants. The most effective means of accomplishing this gene silencing is through the use of viral vectors, with the Tobacco Ratt
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Contact: David Crotty
crotty@cshl.edu
516-422-4007
Cold Spring Harbor Laboratory
Source:Eurekalert

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