Navigation Links
Cold Spring Harbor Protocols features stem cell differentiation, plant RNAi methods
Date:2/2/2009

COLD SPRING HARBOR, N.Y. (Mon., Feb. 2, 2009) -- By using OP9-DL1 cells as a support system, researchers can study the differentiation of embryonic stem cells into mature components of the immune system. This month's issue of Cold Spring Harbor Protocols (www.cshprotocols.org/TOCs/toc2_09.dtl) features a set of methods from Juan Carlos Ziga-Pflcker's laboratory at the University of Toronto (http://www.immunology.utoronto.ca/faculty/directory/zunigapflucker.htm) detailing "The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro." The article contains a series of protocols describing the establishment, maintenance, and storage of OP9 and OP9-DL1 cells; the co-culture of these cells with embryonic stem cells or hematopoietic stem cells from fetal liver or bone marrow; and the in vitro differentiation of the stem cells into lymphocytes. The OP9-DL1 system has been useful in addressing questions about the cellular and molecular regulation of T-lymphocyte lineage commitment, pre-T cell receptor signaling (beta-selection), functional characteristics of progenitor T cells, and maturation of functional CD8 T cells. The article is freely accessible on the website for Cold Spring Harbor Protocols (http://www.cshprotocols.org/cgi/content/full/2009/2/pdb.prot5156).

The second featured article for February comes from S.P. Dinesh-Kumar and colleagues at Yale University (http://plantfunctionalgenomics.yale.edu/). RNA interference (RNAi) has become an effective tool for the down-regulation of genes in plants. The most effective means of accomplishing this gene silencing is through the use of viral vectors, with the Tobacco Rattle Virus (TRV) providing the most robust results. "Virus-Induced Gene Silencing as a Tool for Delivery of dsRNA into Plants" outlines a simple procedure for introducing TRV-based vectors into commonly studied plants such as Arabidopsis and tomato. This method is freely accessible on the website for Cold Spring Harbor Protocols (http://www.cshprotocols.org/cgi/content/full/2009/2/pdb.prot5139).


'/>"/>

Contact: David Crotty
crotty@cshl.edu
516-422-4007
Cold Spring Harbor Laboratory
Source:Eurekalert

Related biology news :

1. Springer author and advisory board member wins LOreal UNESCO Women in Science Award
2. Springer expands its portfolio in plant sciences with Phytoparasitica
3. Mothers pass on disease clues to offspring
4. A low dose of caffeine when pregnant may damage the heart of offspring for a lifetime
5. Springer and the Physiological Society of Japan sign publishing agreement
6. Springer looks ahead to the Future City
7. Springer partners with Botanical Society of Korea
8. Springer to publish Fisheries Science as of 2009
9. Eating eggs when pregnant affects breast cancer in offspring
10. Springer launches Food Security
11. International Journal of Social Robotics debuts at Springer
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/4/2017)... --  EyeLock LLC , a leader of iris-based identity ... and Trademark Office (USPTO) has issued U.S. Patent No. ... iris image with a face image acquired in sequence ... th issued patent. "The issuance ... multi-modal biometric capabilities that have recently come to market ...
(Date:3/30/2017)... -- The research team of The Hong Kong Polytechnic ... by adopting ground breaking 3D fingerprint minutiae recovery and matching technology, ... accuracy for use in identification, crime investigation, immigration control, security of ... ... A research team led by Dr ...
(Date:3/28/2017)... 2017 The report "Video Surveillance ... Servers, Storage Devices), Software (Video Analytics, VMS), and Service ... Forecast to 2022", published by MarketsandMarkets, the market was ... projected to reach USD 75.64 Billion by 2022, at ... base year considered for the study is 2016 and ...
Breaking Biology News(10 mins):
(Date:10/11/2017)... Md. (PRWEB) , ... October 11, 2017 , ... ... digital pathology, announced today it will be hosting a Webinar titled, “Pathology is ... Advanced Pathology Associates , on digital pathology adoption best practices and how Proscia ...
(Date:10/11/2017)... ... October 11, 2017 , ... A new study ... in frozen and fresh in vitro fertilization (IVF) transfer cycles. The ... IVF success. , After comparing the results from the fresh and frozen transfer ...
(Date:10/10/2017)... , ... October 10, 2017 , ... ... Center’s FirstHand program has won a US2020 STEM Mentoring Award. Representatives of the ... for Excellence in Volunteer Experience from US2020. , US2020’s mission is to change ...
(Date:10/10/2017)... ... October 10, 2017 , ... ... process optimization firm for the life sciences and healthcare industries, announces a presentation ... San Francisco. , The presentation, “Automating GxP Validation for Agile Cloud Platforms,” will ...
Breaking Biology Technology: