Navigation Links
Cold Spring Harbor Protocols features stem cell differentiation, plant RNAi methods
Date:2/2/2009

COLD SPRING HARBOR, N.Y. (Mon., Feb. 2, 2009) -- By using OP9-DL1 cells as a support system, researchers can study the differentiation of embryonic stem cells into mature components of the immune system. This month's issue of Cold Spring Harbor Protocols (www.cshprotocols.org/TOCs/toc2_09.dtl) features a set of methods from Juan Carlos Ziga-Pflcker's laboratory at the University of Toronto (http://www.immunology.utoronto.ca/faculty/directory/zunigapflucker.htm) detailing "The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro." The article contains a series of protocols describing the establishment, maintenance, and storage of OP9 and OP9-DL1 cells; the co-culture of these cells with embryonic stem cells or hematopoietic stem cells from fetal liver or bone marrow; and the in vitro differentiation of the stem cells into lymphocytes. The OP9-DL1 system has been useful in addressing questions about the cellular and molecular regulation of T-lymphocyte lineage commitment, pre-T cell receptor signaling (beta-selection), functional characteristics of progenitor T cells, and maturation of functional CD8 T cells. The article is freely accessible on the website for Cold Spring Harbor Protocols (http://www.cshprotocols.org/cgi/content/full/2009/2/pdb.prot5156).

The second featured article for February comes from S.P. Dinesh-Kumar and colleagues at Yale University (http://plantfunctionalgenomics.yale.edu/). RNA interference (RNAi) has become an effective tool for the down-regulation of genes in plants. The most effective means of accomplishing this gene silencing is through the use of viral vectors, with the Tobacco Rattle Virus (TRV) providing the most robust results. "Virus-Induced Gene Silencing as a Tool for Delivery of dsRNA into Plants" outlines a simple procedure for introducing TRV-based vectors into commonly studied plants such as Arabidopsis and tomato. This method is freely accessible on the website for Cold Spring Harbor Protocols (http://www.cshprotocols.org/cgi/content/full/2009/2/pdb.prot5139).


'/>"/>

Contact: David Crotty
crotty@cshl.edu
516-422-4007
Cold Spring Harbor Laboratory
Source:Eurekalert

Related biology news :

1. Springer author and advisory board member wins LOreal UNESCO Women in Science Award
2. Springer expands its portfolio in plant sciences with Phytoparasitica
3. Mothers pass on disease clues to offspring
4. A low dose of caffeine when pregnant may damage the heart of offspring for a lifetime
5. Springer and the Physiological Society of Japan sign publishing agreement
6. Springer looks ahead to the Future City
7. Springer partners with Botanical Society of Korea
8. Springer to publish Fisheries Science as of 2009
9. Eating eggs when pregnant affects breast cancer in offspring
10. Springer launches Food Security
11. International Journal of Social Robotics debuts at Springer
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:1/11/2017)... MOINES, Iowa , Jan. 11, 2017 ... industry first with the release of its patent-pending calibration ... quickly and reliably perform calibrations, securely upload data logs ... flexibility for the customer. "Fighting drunk driving ... only for the public at large, but also for ...
(Date:1/6/2017)... Calif. , Jan. 5, 2017  Delta ID ... its iris scanning technology for automotive at CESĀ® 2017. ... GNTX ) to demonstrate the use of iris ... identify and authenticate the driver in a car, and ... during the driving experience. Delta ID and ...
(Date:12/22/2016)... 2016  As part of its longstanding mission to improve ... company, recently released its latest children,s book, titled ... on the topics of inheritance and variation of traits that ... in elementary school classrooms in the US. ... Ariana Killoran , whose previous book with 23andMe, ...
Breaking Biology News(10 mins):
(Date:1/18/2017)... ... January 18, 2017 , ... Thirty-six startup companies in University City ... Pennsylvania Department of Community and Economic Development in 2016 as part of the Keystone ... University City Keystone Innovation Zone and represent the highest number of awards to the ...
(Date:1/18/2017)...  HUYA Bioscience International, (HUYA), the leader in accelerating ... pharmaceutical innovations, announced today a strategic collaboration agreement with ... (referred to as CAS Innovation). The collaboration will focus ... scientists at CAS to meet the medical needs of ... first company to have recognized China,s ...
(Date:1/18/2017)... 2017 /PRNewswire/ - SQI Diagnostics Inc. (TSX-V: SQD; ... that develops and commercializes proprietary technologies and products ... today announced that Cameron Prange , President ... from its Board of Directors.  Mr. Prange,s resignation ... that have limited both his ability to act ...
(Date:1/17/2017)... (PRWEB) , ... January 17, 2017 , ... ... addition of Rheumatoid Factor (RF) to its VALIDATE® SP2 calibration verification / linearity ... in a human serum base. Each VALIDATE® SP2 kit is prepared using the ...
Breaking Biology Technology: