Navigation Links
Cold Spring Harbor Protocols features organ and cell culture methods
Date:11/3/2008

COLD SPRING HARBOR, N.Y. (Mon., Nov. 3, 2008) Understanding the function of organs like the brain, kidney, and reproductive tissues requires experimental systems that allow for the study and manipulation of developing cells and tissues in the laboratory. This month's issue of Cold Spring Harbor Protocols (www.cshprotocols.org/TOCs/toc11_08.dtl) features two articles with detailed instructions for setting up these experimental culture systems. Both methods are derived from Cold Spring Harbor Laboratory's renowned Molecular Embryology of the Mouse course (http://meetings.cshl.edu/courses/c-mous09.shtml).

Neural progenitor cells, which are destined to contribute to the nervous system, are often difficult to identify. "Isolation, Culture and Differentiation of Progenitor Cells from the Central Nervous System," from Larysa Pevny's laboratory at the University of North Carolina (http://www.unc.edu/~pevny/), describes the neurosphere assay, which allows one to identify neural progenitor cells as well as to determine both their self-renewal capacity and their ability to generate three primary cell types of the nervous system: neurons, astrocytes and oligodendrocytes. The protocol is freely accessible on the website for Cold Spring Harbor Protocols (http://www.cshprotocols.org/cgi/content/full/2008/12/pdb.prot5077).

Blanche Capel's laboratory at Duke University (http://www.cellbio.duke.edu/faculty/capel/index.html) provides "Preparing Recombinant Gonad Organ Cultures." It can be useful to assay migration between any two adjacent tissues during development. This protocol assays cell migration between the developing gonad and kidney using tissue recombination, combined with an organ culture technique. This method is freely accessible on the website for Cold Spring Harbor Protocols (http://www.cshprotocols.org/cgi/content/full/2008/12/pdb.prot5078).


'/>"/>

Contact: David Crotty
crotty@cshl.edu
516-422-4007
Cold Spring Harbor Laboratory
Source:Eurekalert

Related biology news :

1. Springer increases publishing activities in South Korea
2. Springer will publish Journal of Coastal Conservation
3. Springer book wins Quality of Communication Award
4. Species still have more viable offspring if they can choose their best mate
5. From brains to behavior: Cold Spring Harbor Protocols features methods for neuroscience research
6. Estuarine Research Federation chooses Springer as publishing partner
7. Springer and the New York Botanical Garden Press join forces to publish botanical journals
8. Cold Spring Harbor Protocols highlights a method that captures cell growth and activity
9. Hellish hot springs yield greenhouse gas-eating bug
10. Springer founds new journal Air Quality, Atmosphere and Health
11. Moms obesity during conception phase may set the stage for offsprings obesity risk
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/9/2016)... , June 9, 2016  Perkotek an innovation leader in attendance control systems ... seamlessly log work hours, for employers to make sure the right employees are actually ... http://photos.prnewswire.com/prnh/20160609/377486LOGO ... ... ...
(Date:6/2/2016)... -- The Department of Transport Management (DOTM) of ... US Dollar project, for the , Supply and ... and IT Infrastructure , to Decatur ... of Identity Management Solutions. Numerous renowned international vendors participated in ... was selected for the most compliant and innovative solution. ...
(Date:5/24/2016)... superior patient care by providing unparalleled technology to leaders of the medical imaging industry. ... recently added to the range of products distributed by Ampronix. Photo - ... ... ... ...
Breaking Biology News(10 mins):
(Date:6/23/2016)... ... 23, 2016 , ... Mosio, a leader in clinical research ... Recruitment and Retention Tips.” Partnering with experienced clinical research professionals, Mosio revisits the ... tools, and strategies for clinical researchers. , “The landscape of how patients receive ...
(Date:6/23/2016)... 2016 /PRNewswire/ - FACIT has announced the creation ... biotechnology company, Propellon Therapeutics Inc. ("Propellon" or "the ... a portfolio of first-in-class WDR5 inhibitors for the ... WDR5 represent an exciting class of therapies, possessing ... for cancer patients. Substantial advances have been achieved ...
(Date:6/23/2016)... ... June 23, 2016 , ... Charm Sciences, Inc. is pleased ... received AOAC Research Institute approval 061601. , “This is another AOAC-RI approval of ... Salter, Vice President of Regulatory and Industrial Affairs. “The Peel Plate methods perform ...
(Date:6/23/2016)... , June 23, 2016   EpiBiome , a ... $1 million in debt financing from Silicon Valley Bank ... automation and to advance its drug development efforts, as ... facility. "SVB has been an incredible strategic ... services a traditional bank would provide," said Dr. ...
Breaking Biology Technology: