Navigation Links
Cold Spring Harbor Protocols features organ and cell culture methods
Date:11/3/2008

COLD SPRING HARBOR, N.Y. (Mon., Nov. 3, 2008) Understanding the function of organs like the brain, kidney, and reproductive tissues requires experimental systems that allow for the study and manipulation of developing cells and tissues in the laboratory. This month's issue of Cold Spring Harbor Protocols (www.cshprotocols.org/TOCs/toc11_08.dtl) features two articles with detailed instructions for setting up these experimental culture systems. Both methods are derived from Cold Spring Harbor Laboratory's renowned Molecular Embryology of the Mouse course (http://meetings.cshl.edu/courses/c-mous09.shtml).

Neural progenitor cells, which are destined to contribute to the nervous system, are often difficult to identify. "Isolation, Culture and Differentiation of Progenitor Cells from the Central Nervous System," from Larysa Pevny's laboratory at the University of North Carolina (http://www.unc.edu/~pevny/), describes the neurosphere assay, which allows one to identify neural progenitor cells as well as to determine both their self-renewal capacity and their ability to generate three primary cell types of the nervous system: neurons, astrocytes and oligodendrocytes. The protocol is freely accessible on the website for Cold Spring Harbor Protocols (http://www.cshprotocols.org/cgi/content/full/2008/12/pdb.prot5077).

Blanche Capel's laboratory at Duke University (http://www.cellbio.duke.edu/faculty/capel/index.html) provides "Preparing Recombinant Gonad Organ Cultures." It can be useful to assay migration between any two adjacent tissues during development. This protocol assays cell migration between the developing gonad and kidney using tissue recombination, combined with an organ culture t
'/>"/>

Contact: David Crotty
crotty@cshl.edu
516-422-4007
Cold Spring Harbor Laboratory
Source:Eurekalert

Page: 1 2

Related biology news :

1. Springer increases publishing activities in South Korea
2. Springer will publish Journal of Coastal Conservation
3. Springer book wins Quality of Communication Award
4. Species still have more viable offspring if they can choose their best mate
5. From brains to behavior: Cold Spring Harbor Protocols features methods for neuroscience research
6. Estuarine Research Federation chooses Springer as publishing partner
7. Springer and the New York Botanical Garden Press join forces to publish botanical journals
8. Cold Spring Harbor Protocols highlights a method that captures cell growth and activity
9. Hellish hot springs yield greenhouse gas-eating bug
10. Springer founds new journal Air Quality, Atmosphere and Health
11. Moms obesity during conception phase may set the stage for offsprings obesity risk
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:7/30/2014)... The analysis of the roughly 3,000 pieces is still ... that the results will be of major significance. "Amazingly ... insect species in Fushun amber that we found in ... , The Baltic amber comes from the Baltic ... Sites rich in finds are, e.g., the coastal regions ...
(Date:7/29/2014)... research by academics in the University of Bristol,s Nutrition ... take liquid calories into account when planning meals. , ... the Study of Ingestive Behavior Conference (SSIB 2014) in ... argues that we do. , The team was led ... School of Experimental Psychology. , As part of a ...
(Date:7/29/2014)... If you,ve ever ordered the wrong food at a restaurant, ... may have less to do with what you want and ... After analyzing 217 menus and the selections of over 300 ... International Journal of Hospitality Management showed that when it ... most: what you see on the menu and how you ...
Breaking Biology News(10 mins):Chinese mosquitos on the Baltic Sea 2Menu secrets that can make you slim by design 2
... Professor Hannes Lohi,s research group at the University of ... in ITGA10 gene, causing chondrodysplasia in two dog breeds, ... research revealed a new chondrodysplasia gene in dogs, and ... implications on bone biology as well as canine health. ...
... Huonglongbing, or citrus greening, looms darkly over the United ... whose fresh fruit alone was valued at more than ... team led by a University of California, Davis, plant ... picture of how citrus greening impacts trees before they ...
... that expecting a reward or punishment can affect brain ... sight or touch. For example, research shows that these ... are expecting a treat. However, researchers know less about ... expected reward is denied. Insight on these scenarios can ...
Cached Biology News:New dwarfism mutation identified in dogs 2New study offers hope for halting incurable citrus disease 2New study offers hope for halting incurable citrus disease 3Getting an expected award music to the brain's ears 2
(Date:7/30/2014)... 30, 2014 LayerBio, Inc., an MIT ... its first round of seed financing from friends and ... efforts to develop a novel sustained-release product for ophthalmology. ... investors and their commitment to this important work," said ... is one of many areas in which innovations in ...
(Date:7/29/2014)... (PRWEB) July 30, 2014 “PharmaSphere: ... Equity and Venture Capital Funding” report provides in-depth ... the private equity and venture capital segments of ... are expected to have significant impact on the ... and globally. Furthermore, the report identifies regions of ...
(Date:7/29/2014)... 29, 2014 The advanced optical methods ... that are driving a revolution in the neurosciences were ... journal published by SPIE, the international society for ... with the launch of the BRAIN Initiative in the ... lay out a vision for and provide examples of ...
(Date:7/29/2014)... 29, 2014 Human Longevity, Inc. (HLI), a ... on extending the healthy, high performance human life span, ... Ph.D., an expert in machine learning and machine translation ... HLI from Google where he was Distinguished Research Scientist ... directly to HLI Co-Founder and CEO, J. Craig ...
Breaking Biology Technology:Strategic Trends of Biotech Investment Industry (Private Equity and Venture Capital Funding) in New Report Available at MarketOptimizer.org 2Strategic Trends of Biotech Investment Industry (Private Equity and Venture Capital Funding) in New Report Available at MarketOptimizer.org 3Strategic Trends of Biotech Investment Industry (Private Equity and Venture Capital Funding) in New Report Available at MarketOptimizer.org 4SPIE Launches 'Neurophotonics' Journal with Special Section on BRAIN Initiative 2SPIE Launches 'Neurophotonics' Journal with Special Section on BRAIN Initiative 3SPIE Launches 'Neurophotonics' Journal with Special Section on BRAIN Initiative 4Franz Och, Ph.D., Expert in Machine Learning and Machine Translation, Joins Human Longevity, Inc. as Chief Data Scientist 2Franz Och, Ph.D., Expert in Machine Learning and Machine Translation, Joins Human Longevity, Inc. as Chief Data Scientist 3
... , ... a leading developer of innovative research productivity tools, announces the release of FreezerPro MultiSite, ... multiple, independent labs on a single instance of FreezerPro, facilitating IT resources, simplifying administration ... ...
... May 20 L.J. Star , ... flow indicators, announced that it has a suite of solutions for the ... , , ... to connect and visually monitor fluid flows, and thereby help maintain strict ...
... H. Chon, PhD, head of the Department of Biomedical ... Snehraj Merchant of The ScottCare Corporation, makes it possible ... of atrial fibrillation (AF), the most common form of ... algorithm, utilized in the new ScottCare CardioView Dx Suite, ...
Cached Biology Technology:RURO Launches FreezerPro MultiSite 2L.J. Star Announces Stainless Sanitary Fittings, Sight Glasses and Sight Flow Indicators for the Special Demands of the Pharmaceutical Industry 2L.J. Star Announces Stainless Sanitary Fittings, Sight Glasses and Sight Flow Indicators for the Special Demands of the Pharmaceutical Industry 3L.J. Star Announces Stainless Sanitary Fittings, Sight Glasses and Sight Flow Indicators for the Special Demands of the Pharmaceutical Industry 4Detection of potentially deadly atrial fibrillation dramatically improved by new algorithm 2Detection of potentially deadly atrial fibrillation dramatically improved by new algorithm 3
CQCS kit for first KR 4i , (first unit)....
... 384, compatible with hood ... handling system for 384-well ... accurately prepares large quantities ... The Wellpro also performs ...
...
...
Biology Products: