Navigation Links
Cold Spring Harbor Protocols features chromosomal rearrangement, gene copy number methods
Date:9/1/2010

COLD SPRING HARBOR, N.Y. (Wed., Sept. 1, 2010) -- A cell devotes a significant amount of effort to maintaining the stability of its genome, preventing the sorts of chromosomal rearrangements characteristic of many cancers. Assays that measure the rate of gross chromosomal rearrangements (GCRs) are needed in order to understand the individual genes and the different pathways that suppress genomic instability. In the September issue of Cold Spring Harbor Protocols, Richard Kolodner and colleagues from the University of California, San Diego's Ludwig Institute for Cancer Research present "Determination of Gross Chromosomal Rearrangement Rates," a genetic assay to quantitatively measure the rate at which GCRs occur in yeast cells. The assay measures the rate of simultaneous inactivation of two markers placed on a nonessential end of a yeast chromosome. This simple protocol for determining GCR mutation rates in a variety of genetic backgrounds coupled with a diversity of modified GCR assays has provided tremendous insight into the large numbers of pathways that suppress genomic instability in yeast and appear to be relevant to cancer suppression pathways in humans. This featured protocol is freely available on the journal's website.

Large segments of DNA can vary in copy number between individuals. Such copy number variations (CNVs) contribute greatly to genetic diversity and are also thought to be associated with susceptibility or resistance to some diseases, including cancer. "Simple Copy Number Determination with Reference Query Pyrosequencing (RQPS)," featured in the September issue of Cold Spring Harbor Protocols, provides an assay for determining the copy number of any allele in the genome. The method, from Raphael Kopan and colleagues at Washington University, takes advantage of the fact that pyrosequencing can accurately measure the ratio of DNA fragments in a mixture that differ by a single nucleotide. A reference allele with a known copy number and a query allele with an unknown copy number are engineered with single nucleotide variations, and the ratio seen between these probes and genomic DNA reflects the copy number. RQPS can be used to measure copy number of any transgene, differentiate homozygotes from heterozygotes, detect the CNV of endogenous genes, and screen embryonic stem cells targeted with bacterial artificial chromosome (BAC) vectors. RQPS is rapid, inexpensive, sensitive, and adaptable to high-throughput approaches. The article is freely available on the journal's website.


'/>"/>

Contact: David Crotty
crotty@cshl.edu
516-422-4007
Cold Spring Harbor Laboratory
Source:Eurekalert

Related biology news :

1. Cold Spring Harbor Laboratory scientists trace a novel way cells are disrupted in cancer
2. Cold Spring Harbor Protocols features organ and cell culture methods
3. International Journal of Social Robotics debuts at Springer
4. Springer launches Food Security
5. Eating eggs when pregnant affects breast cancer in offspring
6. Springer to publish Fisheries Science as of 2009
7. Springer partners with Botanical Society of Korea
8. Springer looks ahead to the Future City
9. Springer and the Physiological Society of Japan sign publishing agreement
10. A low dose of caffeine when pregnant may damage the heart of offspring for a lifetime
11. Mothers pass on disease clues to offspring
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:2/9/2016)... Calif. , Feb. 9, 2016 Vigilant Solutions ... license plate reader (LPR) to develop a lead in a ... commercially available LPR data to locate the suspect vehicle. Due ... of the case have been omitted at the agency,s request. ... for the agency explains, "Our victim was found deceased at ...
(Date:2/3/2016)... --> --> ... Fingerprint Identification System Market by Component (Hardware and Software), ... Finance, Government, Healthcare, and Transportation) and Geography - Global ... expected to be worth USD 8.49 Billion by 2020 ... 2020. The transformation and technology evolution from the manual ...
(Date:2/2/2016)... , Feb. 2, 2016 Checkpoint ... that Rising Market Are you interested in ... forecasts revenues for checkpoint inhibitors. Visiongain,s report gives ... submarket, product and national level. Avoid falling ... what progress, opportunities and revenues those emerging cancer ...
Breaking Biology News(10 mins):
(Date:2/8/2016)... , February 8, 2016 ... Private Limited, an innovation-driven oncology company developing next ... less toxic, today announced that chairman emeritus of ... invested in the company as part of the ... joining existing investors Navam Capital and Aarin Capital. ...
(Date:2/8/2016)... 2016  BioElectronics Corporation (OTC Pink: BIEL), the ... that it is responding to a notice of ... and Exchange Commission posted on the agency website.  ... the Board of BioElectronics Corporation and the Edward ... The Fuqua School of Business, Duke University.   ...
(Date:2/8/2016)... (PRWEB) , ... February 08, 2016 , ... ... (AI) and leading supplier of Semantic Graph Database technology, today announced the availability ... release of Cloudera Enterprise through the Cloudera Certified Technology Program (CCPT). ...
(Date:2/8/2016)... , Feb. 8, 2016  Diplomat Pharmacy, Inc. (NYSE: DPLO) announced today that its new ... specialty pharmacy resource–user-centric, story-driven, knowledge-based and mobile-friendly. Visit ... ... ... "The goal was ...
Breaking Biology Technology: