Navigation Links
Cold Spring Harbor Protocols features RNA analysis methods
Date:10/1/2009

COLD SPRING HARBOR, N.Y. (Thurs., Oct. 1, 2009) The study of RNA has long been the tool of choice for understanding where and when genes are expressed in a cell, tissue, or organism during development or under specific physiological or environmental conditions. Recent discoveries have revolutionized our concept of RNA function; it is now known to be active in a much wider set of biological processes than was previously believed. Techniques for isolating RNA and for uncovering its interactions with proteins have taken on new importance as many laboratories define the roles of specific RNAs in the cell. The October issue of Cold Spring Harbor Protocols (www.cshprotocols.org/TOCs/toc10_09.dtl) features two articles detailing methods for RNA analysis in zebrafish and the worm C. elegans.

Quantitative Real-Time RT-PCR (qRT-PCR) of Zebrafish Transcripts: Optimization of RNA Extraction, Quality Control Considerations and Data Analysis from Donald Love and colleagues at the University of Auckland (http://www.sbs.auckland.ac.nz/uoa/science/about/departments/sbs/research/molecular-cellular/love-don/associate-professor-don-love.cfm) presents an optimized method for RNA isolation from zebrafish, along with quality assessment and the use of reference genes. A protocol for quantitative real-time polymerase chain reaction (qRT-PCR) is also included. The article is freely available on the website for Cold Spring Harbor Protocols (http://cshprotocols.cshlp.org/cgi/content/full/2009/10/pdb.prot5314).

RNA molecules interact with proteins to drive many cellular activities, including post-transcriptional processing of RNA, regulation of tra
'/>"/>

Contact: David Crotty
crotty@cshl.edu
516-422-4007
Cold Spring Harbor Laboratory
Source:Eurekalert

Page: 1 2

Related biology news :

1. 2 neuroscientists from Cold Spring Harbor Laboratory awarded transformative NIH grants
2. Early spring time for Edinburgh? Study predicts effect of global warming on spring flowers
3. Methods for gene transfer in stem cells featured in Cold Spring Harbor Protocols
4. Spring cold snap helps with stream ecosystem research
5. Springer to collaborate with the Human Genome Organization
6. Springer launches SpringerImages
7. Battle of the sexes benefits offspring, says research
8. Bisphenol A exposure in pregnant mice permanently changes DNA of offspring
9. Springer Series editor John Smol receives two prestigious biology awards
10. Resilin springs simplify the control of crustacean limb movements
11. Spring agricultural fires have large impact on melting Arctic
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:10/15/2014)... a non-traditional biophysics textbook and it describes how life ... of discovery into biological systems and provides an understanding ... is about how our genes make proteins that control ... cells in an organism. It quantifies the principles of ... be found on both large and small scales. The ...
(Date:10/15/2014)... to an unexpected extent. The outbreak does not follow ... shows a new disease dynamic in regions, where it ... German National Academy of Sciences Leopoldina, acatech – the ... of the German Academies of Sciences and Humanities have ... In the statement the academies call for the following ...
(Date:10/15/2014)... Oct. 15, 2014 NXT-ID, Inc. (NASDAQ: NXTD ... focused on the growing mobile commerce market releases photos and video ... Square on Monday October 13 th . ... Tunnel and angel investor Mr. Chad A. Verdi ... of NXT-ID thanked his investors and employees "for their work and ...
Breaking Biology News(10 mins):New book about life as seen from physics 2Academies call for consequences from the Ebola virus epidemic 2Academies call for consequences from the Ebola virus epidemic 3Academies call for consequences from the Ebola virus epidemic 4Academies call for consequences from the Ebola virus epidemic 5Photo Release: NXT-ID Inc. Rings Opening Bell of the NASDAQ Stock Market October 13th 2Photo Release: NXT-ID Inc. Rings Opening Bell of the NASDAQ Stock Market October 13th 3
... release is available in Spanish . , ... vectors of equine piroplasmosis in horses in a 2009 Texas ... The United States has been considered free from the disease ... In October 2009, in Kleberg County, Texas, a mare was ...
... use hot enough water to eliminate dangerous bacteria like ... from hospital uniforms, according to a study published in ... Epidemiology , the journal of the Society for Healthcare ... researchers from University College in London, was prompted by ...
... More than three-quarters of cancer patients have insufficient levels of ... with more advanced cancer, according to a study presented on ... American Society for Radiation Oncology (ASTRO). , "Until recently, ... on the prognosis or course of cancer. Researchers are just ...
Cached Biology News:Tick responsible for equine piroplasmosis outbreak identified 2Study: Residential washers may not kill hospital-acquired bacteria 2Vitamin D deficiency common in cancer patients 2
(Date:10/20/2014)... 20, 2014 OncLive® ... Cancer Center at Thomas Jefferson University has joined ... the Strategic Alliance Partnership program, the Sidney Kimmel ... to raise awareness of the Center’s cutting-edge research ... projects. Clinicians and other health care professionals from ...
(Date:10/19/2014)... 19, 2014 The Latin American hardware ... in Latin America with analysis and forecast of revenue. ... and is expected to reach $2,366.8 million by 2018, ... , Browse through the TOC of the Latin American ... the in-depth analysis provided. It also provides a glimpse ...
(Date:10/19/2014)... The Asia-Pacific Bromine Market report defines ... analysis and forecast of revenue. , Browse through ... report, to get an idea of the in-depth ... the segmentation in the Asia-Pacific bromine market, and ... , http://www.micromarketmonitor.com/market/asia-pacific-bromine-6741503144.html , Bromine is a ...
(Date:10/19/2014)... report "Chocolate, Cocoa Beans, Lecithin, Sugar and Vanilla Market ... (2011 - 2016)," analyzes the chocolate market by ... market drivers, restraints, and opportunities for the chocolate market ... chocolate market is expected to grow from $83.2 billion ... CAGR of 2.7% from 2011 to 2016. , Get ...
Breaking Biology Technology:Sidney Kimmel Cancer Center at Thomas Jefferson University Partners With OncLive 2Sidney Kimmel Cancer Center at Thomas Jefferson University Partners With OncLive 3The Latin American hardware encryption display market is expected to reach $2,366.8 million by 2018 - New Report by MicroMarket Monitor 2The Latin American hardware encryption display market is expected to reach $2,366.8 million by 2018 - New Report by MicroMarket Monitor 3The Asia-Pacific Bromine Market is estimated to grow to $4,080.1 million by 2018 - New Report by MicroMarket Monitor 2The Asia-Pacific Bromine Market is estimated to grow to $4,080.1 million by 2018 - New Report by MicroMarket Monitor 3Chocolate Market Projected To Reach $98.3 Billion by 2016 - New Report by MarketsandMarkets 2Chocolate Market Projected To Reach $98.3 Billion by 2016 - New Report by MarketsandMarkets 3Chocolate Market Projected To Reach $98.3 Billion by 2016 - New Report by MarketsandMarkets 4
... From the structure of DNA to nautical rope ... as they are useful in nature and manufacturing alike. ... Sciences (SEAS) have discovered a way to synthesize and ... into helical clusters and have further demonstrated the fabrication ...
... Jan. 8 InteKrin Therapeutics, Inc.,will present at the ... Pacific Time on Tuesday, January 13, 2009, to be ... InteKrin Therapeutics, President and,CEO Denny Lanfear will present at ... ( http://www.InteKrin.com ): , InteKrin ...
... Public Health Physician Spent Last 10 Years Helping Implement ... Abt Associates announced that Dr. John Osika has ... and Infectious Disease Advisor. Dr. Osika, a board ... is a recognized public health expert with extensive experience ...
Cached Biology Technology:Researchers control the assembly of nanobristles into helical clusters 2InteKrin Therapeutics to Present at the 27th Annual JP Morgan Healthcare Conference on Tuesday, January 13 2Dr. John Stephen Osika, HIV/AIDS and Infectious Disease Expert, Joins Abt Associates 2
... number is a new product ... Cornings product number. If showing ... under the old Sigma-Aldrich number ... for assistance. ID clarifier: barcoded, ...
... mat made from an inert polymer with a ... 384 split septum caps for use with ... for PCR when used in conjunction with screw ... with standard pipetting equipment 384 septum caps ...
Packaging: package with 500 l Patent/trademark/license (electronic): (r) Registered trademark of Molecular Probes...
... unique alternative to traditional TBE (TRIS ... buffers. Bionic Buffer allows for higher ... and high-resolution band separation. -Application: Ready ... dilution to working 1x concentrations. ...
Biology Products: