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Cold Spring Harbor Protocols features Drosophila neurobiology methods
Date:7/1/2010

e role of stem cells in development. These neural stem cells, called "neuroblasts," have provided insight into the role of cell polarity in influencing cell fate. Identifying neuroblasts and their progeny requires a method capable of recognizing cell polarity and cell fate markers. "Immunofluorescent Staining of Drosophila Larval Brain Tissue," provided by Cheng-Yu Lee and colleagues, describes procedures for the collection and processing of Drosophila larval brains for analysis of these markers. Neuroblasts are identified via immunolocalization, the use of labeled antibodies that specifically bind the marker proteins of interest. The article is featured in the July issue of Cold Spring Harbor Protocols and is freely available on the journal's website.


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Contact: David Crotty
crotty@cshl.edu
516-422-4007
Cold Spring Harbor Laboratory
Source:Eurekalert

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