Navigation Links
Cold Spring Harbor Protocols features Drosophila neurobiology methods
Date:7/1/2010

COLD SPRING HARBOR, N.Y. (Thurs., July 1, 2010) -- Since the early days of the 20th century and Thomas Hunt Morgan's famous "Fly Room" at Columbia University, the fruit fly Drosophila melanogaster has been at the forefront of biological research. The powerful arsenal of experimental methods developed for this model organism is now being used to tackle one of the great scientific challenges of a new century: understanding the nervous system. Cold Spring Harbor Laboratory's Neurobiology of Drosophila course (http://meetings.cshl.edu/courses/c-dros10.shtml) has served as the training ground for a generation of scientists tackling these complex problems. A new laboratory manual based on the protocols and background information taught in the course promises to spread these techniques to a wider audience. Two of the methods from the manual are featured in the July issue of the journal Cold Spring Harbor Protocols.

When a fly is confronted with danger, it jumps into the air and flies away. The giant fiber system (GFS) of Drosophila is a neuronal circuit that mediates this escape response. The neurons in the GFS are readily identified and easily accessible for experimental assay. "Electrophysiological Recordings from the Drosophila Giant Fiber System," from Marcus Allen and Tanja Godenschwege, describes a simple procedure for stimulating neurons directly in the brain of the adult fly and obtaining recordings from the output muscles of the GFS. The protocol is freely available on the journal's website .

The larval Drosophila brain has been a valuable model for investigating the role of stem cells in development. These neural stem cells, called "neuroblasts," have provided insight into the role of cell polarity in influencing cell fate. Identifying neuroblasts and their progeny requires a method capable of recognizing cell polarity and cell fate markers. "Immunofluorescent Staining of Drosophila Larval Brain Tissue," provided by Cheng-Yu Lee and colleagues, describes procedures for the collection and processing of Drosophila larval brains for analysis of these markers. Neuroblasts are identified via immunolocalization, the use of labeled antibodies that specifically bind the marker proteins of interest. The article is featured in the July issue of Cold Spring Harbor Protocols and is freely available on the journal's website.


'/>"/>

Contact: David Crotty
crotty@cshl.edu
516-422-4007
Cold Spring Harbor Laboratory
Source:Eurekalert

Related biology news :

1. Do eggs matured in the laboratory result in babies with Large Offspring syndrome?
2. Electrophoresis of RNA, avian imaging featured in Junes Cold Spring Harbor Protocols
3. Montana State grad student to study unique soil around Yellowstone hot springs
4. Mays Cold Spring Harbor Protocols features plant gene expression methods, Xenopus imaging
5. Study: Darwin was right to worry that marriage to his cousin affected his offspring
6. Tissue engineering, imaging neuronal circuits featured in Cold Spring Harbor Protocols
7. Top microbiology experts meet in Edinburgh at SGM Spring Conference 2010
8. Stress during pregnancy may increase offsprings risk of asthma
9. Springer editorial board member receives Humboldt Research Award
10. Protein-bait interactions, display libraries featured in Cold Spring Harbor Protocols
11. Beewolves protect their offspring with antibiotics
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:5/6/2017)... SINGAPORE , May 5, 2017 ... has just announced a new breakthrough in biometric ... that exploits quantum mechanical properties to perform ... new smart semiconductor material created by Ram Group ... across finance, entertainment, transportation, supply chains and security. ...
(Date:4/17/2017)... 17, 2017 NXT-ID, Inc. (NASDAQ: NXTD ... filing of its 2016 Annual Report on Form 10-K on Thursday ... ... available in the Investor Relations section of the Company,s website at ... website at http://www.sec.gov . 2016 Year Highlights: ...
(Date:4/11/2017)... Research and Markets has announced the addition of the ... ... grow at a CAGR of 30.37% during the period 2017-2021. ... prepared based on an in-depth market analysis with inputs from industry ... over the coming years. The report also includes a discussion of ...
Breaking Biology News(10 mins):
(Date:5/22/2017)... ... May 22, 2017 , ... Cancer diagnostics and ... booth B2 at the Association for Pathology Informatics Annual Summit at ... to demonstrating its Cancer Diagnostic Cockpit and Consultation Portal, Inspirata will present research ...
(Date:5/21/2017)... ... May 20, 2017 , ... CNSDose is ... trial and error process by finding the right antidepressant faster. CNSDose speeds ... doctor-patient relationship through a personalized approach to treatment. , A peer-reviewed ...
(Date:5/19/2017)... ... 19, 2017 , ... The University City Science Center is ... for commercialization, and who are affiliated with the 21 partner academic and research ... now in its tenth round, is the first multi-institutional proof-of-concept program for the ...
(Date:5/18/2017)... ... May 18, 2017 , ... ... The Tapas Cooking Challenge is a two-hour team-building package designed for groups of ... by Chef Jodi Abel, which include items, such as Blackened Shrimp with Edamame ...
Breaking Biology Technology: