Navigation Links
Cold Spring Harbor Protocols features Drosophila neurobiology methods
Date:7/1/2010

COLD SPRING HARBOR, N.Y. (Thurs., July 1, 2010) -- Since the early days of the 20th century and Thomas Hunt Morgan's famous "Fly Room" at Columbia University, the fruit fly Drosophila melanogaster has been at the forefront of biological research. The powerful arsenal of experimental methods developed for this model organism is now being used to tackle one of the great scientific challenges of a new century: understanding the nervous system. Cold Spring Harbor Laboratory's Neurobiology of Drosophila course (http://meetings.cshl.edu/courses/c-dros10.shtml) has served as the training ground for a generation of scientists tackling these complex problems. A new laboratory manual based on the protocols and background information taught in the course promises to spread these techniques to a wider audience. Two of the methods from the manual are featured in the July issue of the journal Cold Spring Harbor Protocols.

When a fly is confronted with danger, it jumps into the air and flies away. The giant fiber system (GFS) of Drosophila is a neuronal circuit that mediates this escape response. The neurons in the GFS are readily identified and easily accessible for experimental assay. "Electrophysiological Recordings from the Drosophila Giant Fiber System," from Marcus Allen and Tanja Godenschwege, describes a simple procedure for stimulating neurons directly in the brain of the adult fly and obtaining recordings from the output muscles of the GFS. The protocol is freely available on the journal's website .

The larval Drosophila brain has been a valuable model for investigating the role of stem cells in development. These neural stem cells, called "neuroblasts," have provided insight into the role of cell polarity in influencing cell fate. Identifying neuroblasts and their progeny requires a method capable of recognizing cell polarity and cell fate markers. "Immunofluorescent Staining of Drosophila Larval Brain Tissue," provided by Cheng-Yu Lee and colleagues, describes procedures for the collection and processing of Drosophila larval brains for analysis of these markers. Neuroblasts are identified via immunolocalization, the use of labeled antibodies that specifically bind the marker proteins of interest. The article is featured in the July issue of Cold Spring Harbor Protocols and is freely available on the journal's website.


'/>"/>

Contact: David Crotty
crotty@cshl.edu
516-422-4007
Cold Spring Harbor Laboratory
Source:Eurekalert

Related biology news :

1. Do eggs matured in the laboratory result in babies with Large Offspring syndrome?
2. Electrophoresis of RNA, avian imaging featured in Junes Cold Spring Harbor Protocols
3. Montana State grad student to study unique soil around Yellowstone hot springs
4. Mays Cold Spring Harbor Protocols features plant gene expression methods, Xenopus imaging
5. Study: Darwin was right to worry that marriage to his cousin affected his offspring
6. Tissue engineering, imaging neuronal circuits featured in Cold Spring Harbor Protocols
7. Top microbiology experts meet in Edinburgh at SGM Spring Conference 2010
8. Stress during pregnancy may increase offsprings risk of asthma
9. Springer editorial board member receives Humboldt Research Award
10. Protein-bait interactions, display libraries featured in Cold Spring Harbor Protocols
11. Beewolves protect their offspring with antibiotics
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:2/1/2016)... , Feb. 1, 2016  Today, the first ... (AHA) announced plans to develop a first of its ... power of IBM Watson. In the first application of ... IBM (NYSE: IBM ), and Welltok will create ... health assessments with cognitive analytics, delivered on Welltok,s health ...
(Date:1/25/2016)... , Jan. 25, 2016  Glencoe Software, the world-leading ... and publication industries, will provide the data management solution ... (NPSC). ... Phenotypic analysis measures the ... organisms, allowing comparisons between states such as health and ...
(Date:1/20/2016)... Jan. 20, 2016   MedNet Solutions , an ... spectrum of clinical research, is pleased to announce the ... achievements are the result of the company,s laser focus ... eClinical , it,s comprehensive, easy-to-use and highly affordable ... --> Key MedNet growth achievements in 2015 include: ...
Breaking Biology News(10 mins):
(Date:2/11/2016)... , Feb. 11, 2016  Spectra BioPharma Selling ... (CSO) that provides biopharma companies the experience, expertise, ... and deploy outsourced sales teams. Created in concert ... addresses both the strategic and tactical needs of ... sales solutions through both personal and non-personal promotion. ...
(Date:2/11/2016)... BioInformant announces the February 2016 release of its ... and Technologies – Market Size, Segments, Trends, and Projections ... The first and only market research firm ... more than a decade of historical information on all ... type. This powerful 175 page global strategic report contains ...
(Date:2/10/2016)... WASHINGTON , February 10, 2016 Early-career ... , Peru , Uganda ... their life-enhancing work in health and nutrition   Indonesia ... Uganda and Yemen are ... sciences and epidemiology. They are also celebrated for mentoring young women scientists ...
(Date:2/10/2016)... (NYSE MKT: ISR), a medical technology company and innovator ... treatment of prostate, brain, lung, head and neck and ... second quarter and six months of fiscal 2016, which ... --> Revenue was $1.19 million for ... 31, 2015, a 12% increase compared to $1.07 million ...
Breaking Biology Technology: