Navigation Links
Click chemistry with copper -- a biocompatible version
Date:7/18/2011

the co-leader of this research with Peng Wu, of the Albert Einstein College of Medicine.

"While copper-free click chemistry may have advantages for whole animal imaging experiments such as imaging in mice," Liu says, "our ligand-accelerated copper reaction is better suited for enriching glycoproteins for their identification."

The ligand-accelerated copper-catalyzed reaction was used to label glycans in recombinant glycoproteins, glycoproteins in cell lysates, glycoproteins on live cell surfaces, and glycoconjugates in live zebrafish embryos. Because a zebrafish embryo is transparent in the first 24 hours of its development, it allows labeled glycans to be detected via molecular imaging techniques, making it a highly useful model for developmental biology studies.

"Based on our results," says Peng Wu, "we believe that ligand-accelerated copper-catalyzed click chemistry represents a powerful and highly adaptive bioconjugation tool that holds great promise for further improvement with the discovery of more versatile catalyst systems."

Click chemistry, which was introduced in 2002 by the Nobel laureate chemist Barry Sharpless of the Scripps Research Institute, utilizes a copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction that makes it possible for certain chemical building blocks to "click" together in an irreversible linkage, analogous to the snapping together of Lego blocks. While the technique immediately proved valuable for attaching small molecular probes to various biomolecules in a test tube or on fixed cells, it could not be used for biomolecule labeling in live cells or organisms because of the copper catalyst.

In 2007, Carolyn Bertozzi, a chemist who holds joint appointments with Berkeley Lab, the University of California (UC) Berkeley, and the Howard Hughes Medical Institute, led a research effort that produced a copper-free version of click chemistry. In this version, glycans were metabolically
'/>"/>

Contact: Lynn Yarris
lcyarris@lbl.gov
510-486-5375
DOE/Lawrence Berkeley National Laboratory
Source:Eurekalert  

Page: 1 2 3

Related biology news :

1. Diagnosing heart attacks may be a lick and a click away
2. Low dose naltrexone (LDN): Harnessing the bodys own chemistry to treat human ovarian cancer
3. Obese dieters brain chemistry works against their weight-loss efforts
4. NIST tunes metasurface with fluid in new concept for sensing and chemistry
5. U of Alberta research group recognized with national chemistry awards
6. Healthy dose of chemistry coming to Montreal
7. Nobel Laureate to speak at American Chemical Society green chemistry conference
8. First Washington green chemistry conference aims to plan for toxics
9. New understanding of brain chemistry could prevent brain damage after injury
10. Chemistry researchers receive patent for new scientific measurement instrument
11. Sweet chemistry: Carbohydrate adhesion gives stainless steel implants beneficial new functions
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Click chemistry with copper -- a biocompatible version
(Date:9/29/2014)... Scientists at Washington State University have concluded that ... apples may help prevent disorders associated with ... to assess these compounds in apple cultivars grown ... edition of the journal Food Chemistry ., ... good source of these nondigestible compounds but there ...
(Date:9/29/2014)... review article published online in Behavioral Brain Research ... the brain as a result of chronic alcohol exposure ... , Clinical assessments and research indicate that individuals ... sleep. This can occur when people are actively drinking, ... are abstaining. , "Sleep-wake disturbances can last for ...
(Date:9/29/2014)... of Pangea was starting to break up and dog-sized dinosaurs ... called phytosaurs and rauisuchids were at the top of the ... predators didn,t interact much as the former was king of ... ideas are changing, thanks largely to the contents of a ... in the German journal Naturwissenschaften , Stephanie ...
Breaking Biology News(10 mins):An apple a day could keep obesity away 2Tooth buried in bone shows prehistoric predators tangled across land, sea 2
... MARC (Minority Access to Research Careers) Program has announced the ... of Reproduction (SSR) Annual Meeting in Portland, Oregon from July ... to promote the entry of underrepresented minority students, postdoctorates and ... to encourage the participation of young scientists at the 2011 ...
... lose weight and suspect your body,s working against you, you may ... Obesity . "When obese persons reduce their food intake ... They may have to work harder and go slower in order ... in the U of I College of Medicine and a member ...
... Australia,s fruit and vegetable industry are under threat, ... to find new ways to control a major ... Associate Professor Anthony Clarke, from QUT,s Faculty of ... largest team of university researchers examining non-chemical based ...
Cached Biology News:Obese dieters' brain chemistry works against their weight-loss efforts 2Qld fruit fly scientists in race against time 2Qld fruit fly scientists in race against time 3
(Date:9/29/2014)... food security, emerging diseases and biodiversity loss requires ... in Science Express that was co-authored ... College of Agriculture and Life Sciences. , For ... scientists has reviewed progress in addressing a broad ... management using approaches that consider evolutionary histories and ...
(Date:9/29/2014)... , Sept. 29, 2014 Research ... "Process Liquid Analyzer, Gas Analyzer, and Gas Chromatograph Market ... & Region - Global Trends & Forecast to 2013 ... A process analyzer is a process analytical instrument ... real-time. It has redefined the way by which companies ...
(Date:9/29/2014)... Transparency Market Research, in its report titled ‘Peptide ... Application - Cancer, Metabolic, CVS, CNS & others, by ... Global Industry Analysis, Size, Share, Growth, Trends and Forecast, ... market was worth US$14.1 billion in 2011 and is ... 8.7% CAGR. , The two main types of peptide ...
(Date:9/29/2014)... 29, 2014  Atlas Venture, an early stage venture ... innovation, today announced that Jason Rhodes , President ... EPZM ), is joining the firm,s life sciences investing ... At Atlas, Rhodes will work closely with the ... an accomplished record in company creation, deal making and ...
Breaking Biology Technology:Evolutionary biology: It's not just for textbooks anymore 2Evolutionary biology: It's not just for textbooks anymore 3Global Process Liquid Analyzer, Gas Analyzer, and Gas Chromatograph (PH/ORP, Chlorine, Oxygen, Carbon dioxide) Market - Trends & Forecast to 2020 2Global Process Liquid Analyzer, Gas Analyzer, and Gas Chromatograph (PH/ORP, Chlorine, Oxygen, Carbon dioxide) Market - Trends & Forecast to 2020 3Global Process Liquid Analyzer, Gas Analyzer, and Gas Chromatograph (PH/ORP, Chlorine, Oxygen, Carbon dioxide) Market - Trends & Forecast to 2020 4Global Peptide Therapeutics Market to be Worth US$25.4 Billion by 2018: Transparency Market Research 2Global Peptide Therapeutics Market to be Worth US$25.4 Billion by 2018: Transparency Market Research 3Atlas Venture Names Jason Rhodes, Veteran Investor and President and CFO of Epizyme, As Partner on Life Sciences Investing Team 2
... Inc. (Nasdaq: ENMD ), a clinical-stage pharmaceutical ... inflammatory diseases, announced today that a Company overview will ... Pharmaceuticals Industry Conference, to be held at the Conference ... Dane R. Saglio, Chief Financial Officer, will present ...
... Systems, Inc., (OTC Bulletin Board: IMDS) a pioneer in ... issuance of its,21st US Patent entitled "Apparatus and Method ... Point Spread,Function of the Detected Light," dated November 4, ... http://www.newscom.com/cgi-bin/prnh/20040412/IMDSLOGO ) , ...
... CRY ), a,biomaterials, medical device and tissue processing company, ... Annual Piper Jaffray Health,Care Conference at The New York Palace ... Anderson, president and chief executive officer of CryoLife,Inc., will provide ... 2:30 p.m. Eastern Time. , ...
Cached Biology Technology:EntreMed to Present at the New York Society of Security Analysts Industry Conference 2Imaging Diagnostic Systems is Recognized for Its Advanced Engineering in Time-Resolved Techniques by the US Patent Office 2Imaging Diagnostic Systems is Recognized for Its Advanced Engineering in Time-Resolved Techniques by the US Patent Office 3CryoLife to Present at 20th Annual Piper Jaffray Health Care Conference 2
... EZ-Spread Plating Beads Sterile plating in ... glass rods and potentially hazardous ethanol flaming, ... choices of ready-to-use sterile packaging: dispenser bottle for ... plating, Fastest and easiest way to ...
The new Silencer siRNA Starter Kit is perfect for researchers new to RNAi. It provides all of the reagents, protocols, and instructions necessary to get underway with siRNA transfection and ensure su...
... a regulated environment, Pipette Tracker Pro ... that automates gravimetric pipette calibration and ... meets todays regulatory compliance requirements. Pipette ... that includes a full security implementation, ...
... the yolk protein Vtg in plasma from juvenile ... biomarker for endocrine disrupting chemicals (EDCs) with estrogenic ... an accepted screening test for the estrogenic effects ... EIA kit is a double-antibody immunometric (sandwich) EIA ...
Biology Products: