Navigation Links
Clemson scientists shed light on molecules in living cells
Date:8/20/2007

CLEMSON, S.C. Clemson University chemists have developed a method to dramatically improve the longevity of fluorescent nanoparticles that may someday help researchers track the motion of a single molecule as it travels through a living cell.

The chemists are exploiting a process called resonance energy transfer, which occurs when fluorescent dye molecules are added to the nanoparticles. Their findings will be reported at the 234th annual national American Chemical Society meeting Aug.19-24 in Boston.

If scientists could track the motion of a single molecule within a living cell it could reveal a world of information. Among other things, scientists could determine how viruses invade a cell or how proteins operate in the body. Such technology also could help doctors pinpoint the exact location of cancer cells in order to better focus treatment and minimize damage to healthy tissue. Outside the body, the technology could help speed up detection of such toxins as anthrax.

The key to developing single-molecule tracking technology may be the development of better fluorescent nanoparticles.

Fluorescent nanoparticles are thousands of times smaller than the width of a human hair and are similar in size to protein molecules, to which they can be attached. When illuminated by a laser beam inside a light microscope equipped with a sensitive digital camera, the nanoparticle attached to a protein will light up, allowing scientists to get a precise fix on the position of the protein and monitor its motion inside a cell.

Until now, nanoparticles have been too dim to detect inside cells, but Clemson chemists have developed a novel type of nanoparticles containing materials called conjugated polymers that light up and stay lit long enough for scientists to string together thousands of images, as in a movie.

Conjugated polymers share many properties with semiconductors like silicon but have the flexibility of plastic. While initial efforts at preparing nanoparticles out of conjugated polymers resulted in particles that were very bright, their brightness quickly faded under the bright glare of a laser beam.

When a conjugated polymer is in a high energy state, it is vulnerable to attack by oxygen, says principal investigator and chemist Jason McNeill. The dye efficiently removes the energy from the molecule and re-emits the energy as light, which greatly improves the brightness and longevity of the nanoparticles.

McNeill says other possible targets of investigation include the formation of plaques and fibrils in the brain associated with Alzheimer's disease and mad cow disease. Graduate students Changfeng Wu, Craig Szymanski, Jennifer Grimland and Yueli Zheng contributed to the study, which the National Science Foundation funded.

Clemson University chemists are presenting 40 papers on a wide range of subjects at the society meeting. Other topics include detection and quantification of uranium in groundwater, conversion of lipid feedstocks such as poultry fat to biodiesel and a new mechanism for antioxidants that fight DNA damage.


'/>"/>

Contact: Susan Polowczuk
spolowc@clemson.edu
864-656-2063
Clemson University
Source:Eurekalert

Related biology news :

1. Wisconsin scientists grow critical nerve cells
2. Scientists ID molecular switch in liver that triggers harmful effects of saturated and trans fats
3. UCSB scientists probe sea floor venting to gain understanding of early life on Earth
4. UAB scientists discover the origin of a mysterious physical force
5. Fox Chase Cancer Center scientists identify immune-system mutation
6. Scientists Replicate Hepatitis C Virus in Laboratory
7. Scientists detect probable genetic cause of some Parkinsons disease cases
8. Scientists find missing enzyme for tuberculosis iron scavenging pathway
9. Scientists seek answers on what activates deadly anthrax spores
10. Yale Scientists Find MicroRNA Regulates Ras Cancer Gene
11. Scientists collaborate to assess health of global environment
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:5/3/2016)... 2016  Neurotechnology, a provider of high-precision biometric ... Biometric Identification System (ABIS) , a complete system ... ABIS can process multiple complex biometric transactions with ... fingerprint, face or iris biometrics. It leverages the ... MegaMatcher Accelerator , which have been used ...
(Date:4/26/2016)... and LONDON , ... Finacle, part of EdgeVerve Systems, a product subsidiary ... Onegini today announced a partnership to integrate the ...      (Logo: http://photos.prnewswire.com/prnh/20151104/283829LOGO ) ... provide their customers enhanced security to access and ...
(Date:4/15/2016)... -- A new partnership announced today will help life ... a fraction of the time it takes today, ... insurance policies to consumers without requiring inconvenient and ... rapid testing (A1C, Cotinine and HIV) and higi,s ... pulse, BMI, and activity data) available at local ...
Breaking Biology News(10 mins):
(Date:6/23/2016)... June 23, 2016 /PRNewswire/ - FACIT has announced ... biotechnology company, Propellon Therapeutics Inc. ("Propellon" ... commercialization of a portfolio of first-in-class WDR5 inhibitors ... such as WDR5 represent an exciting class of ... precision medicine for cancer patients. Substantial advances have ...
(Date:6/23/2016)... YORK , June, 23, 2016  The Biodesign ... to envision new ways to harness living systems and ... Modern Art (MoMA) in New York City ... than 130 participating students, showcased projects at MoMA,s Celeste ... Paola Antonelli , MoMA,s senior curator of architecture ...
(Date:6/23/2016)... , June 23, 2016 Apellis ... Phase 1 clinical trials of its complement C3 ... single and multiple ascending dose studies designed to ... (PD) of subcutaneous injection in healthy adult volunteers. ... (SC) either as a single dose (ranging from ...
(Date:6/23/2016)... ... June 23, 2016 , ... ... and technical consulting, provides a free webinar on Performing Quality Investigations: ... 2016 at 12pm CT at no charge. , Incomplete investigations are still a ...
Breaking Biology Technology: