Navigation Links
Class of antibiotics can enhance gene-silencing tool
Date:7/20/2008

A way to turn off one gene at a time has earned acceptance in biology laboratories over the last decade. Doctors envision the technique, called RNA interference, as a tool to treat a variety of diseases if it can be adapted to humans.

Emory University researchers have discovered that antibiotics known as fluoroquinolones can make RNA interference more effective in the laboratory and reduce potential side effects. The results will be published online this week in the journal Nature Biotechnology.

"The surprising aspect is that some fluoroquinolones have this previously unrecognized property," says senior author Peng Jin, PhD, assistant professor of human genetics at Emory University School of Medicine. "The good part is that doctors have years of experience treating bacterial infections with them, so they are generally considered safe."

The most powerful enhancer of RNA interference was enoxacin, which has been used to treat gonorrhea and urinary tract infections. The group of compounds also includes the widely used antibiotic ciprofloxacin. The antibiotics' effect on RNA interference appears to be chemically separate from their bacteria-killing activities.

Significant barriers still prevent RNA interference from working well in people, Jin says.

"The barriers include specificity and toxicity, as well as getting the RNA to the right place in the body," he says. "If we can enhance how potent a given amount of RNA is and reduce dosage, we're tackling both specificity and toxicity."

Some studies have found that side effects come from the amount of RNA injected, which can trigger an anti-viral response, rather than from the genetic sequence of the RNA used.

Andrew Fire and Craig Mello received the 2006 Nobel Prize in Medicine for their discovery that short pieces of RNA, when introduced into cells, can silence a stretch of genetic code. Artificially introduced RNA hijacks machinery inside the cell called the RNA-induced silencing complex or RISC.

To probe how RISC works, Jin and his co-workers inserted a gene for a fluorescent protein into a cell line, and then added a short piece of RNA that incompletely silences the inserted gene. That way, if a potential drug tweaked the silencing process, the researchers could see it quickly.

They found that enoxacin can increase how well a gene is silenced by up to a factor of ten in cultured cells and by a factor of three in mice. It appears to strengthen the grip of part of RISC, a protein called TRBP, upon small pieces of RNA.


'/>"/>

Contact: Holly Korschun
hkorsch@emory.edu
404-727-3990
Emory University
Source:Eurekalert

Related biology news :

1. World-class environment vision to bring back the species
2. bioMETRX, Inc. Garage Door Opener Named Best In Class
3. Compound has potential for new class of AIDS drugs
4. Cold Spring Harbor Protocols features classic approaches for analyzing chromosomes
5. Nobel laureate Dr. Linda Buck elected to American Academy of Arts & Sciences 2008 Class of Fellows
6. New computer applications allow to classify Iberian pig meat automatically
7. New edition of a classic molecular biology textbook from CSHL Press and Benjamin Cummings
8. World class in Systems Biology is the aim
9. Identification of a novel class of (not-so) small RNAs
10. New class of drug offers hope to treatment-resistant AIDS patients
11. Freshman class sparks start-up company
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/30/2017)... , June 30, 2017 Today, American ... and supplier of face and eye tracking software, ... Product provider program. "Artificial intelligence ... way to monitor a driver,s attentiveness levels while ... being able to detect fatigue and prevent potential ...
(Date:5/16/2017)... May 16, 2017  Veratad Technologies, LLC ( www.veratad.com ... age and identity verification solutions, announced today they will ... 2017, May 15 thru May 17, 2017, in ... International Trade Center. Identity impacts the ... in today,s quickly evolving digital world, defining identity is ...
(Date:4/19/2017)... , April 19, 2017 ... its vendor landscape is marked by the presence of ... is however held by five major players - 3M ... these companies accounted for nearly 61% of the global ... leading companies in the global military biometrics market boast ...
Breaking Biology News(10 mins):
(Date:7/18/2017)... ... July 18, 2017 , ... Recently recognized ... https://dataformsoftware.com ) announces the migration of its flagship cloud-based product Planet Life Cycle ... team-centric, enterprise work management system that merges strategic and financial planning with execution. ...
(Date:7/17/2017)... Yorba Linda, Ca (PRWEB) , ... July 17, ... ... highly heterogeneous and are characterized by a wide range of overlapping clinical features. ... pace of discovery in the field of NDD research and testing. , ...
(Date:7/16/2017)... (PRWEB) , ... July 16, 2017 , ... ... equipment and analytical instruments announced the launch of its new line of Extreme ... ideal for applications that require CO2 and humidity for optimal cell growth such ...
(Date:7/14/2017)... Denville, USA/ Martinsried, Germany (PRWEB) , ... July ... ... a newly developed standardized solution for sample preparation of proteins to clean peptides ... the Diagenode Bioruptor® sonication system. , The PreOmics iST Kit is based ...
Breaking Biology Technology: