Navigation Links
Citrate key in bone's nanostructure
Date:6/8/2011

eak."

After studying bone structure over a five-year period, it was actually serendipitous that Schmidt-Rohr came across a signature that appeared to match what he was seeing.

"We had gotten some crystalline collagen samples to study," he said, "and it turned out that the supplier, Sigma-Aldrich, had used citrate to dissolve the collagen. And the citrate signature in the collagen samples matched the signature we were seeing in bone."

According to Schmidt-Rohr, the role of citrate in bone had been studied up until about 1975, but since that time, no mention was made in any of the newer literature on bone. So in essence, his research team had to rediscover it.

The case for citrate was made most convincingly when graduate research assistant Yanyan Hu was able to extract citrate from cow bone and replace it with carbon 13 (C13) -enriched citrate, resulting in a 30-fold enhancement of the NMR signals of the bone sample. The peaks matched exactly, confirming the presence of citrate on the surface where the apatite nanocrystals had formed.

Schmidt-Rohr further hypothesized that, since citrate is too large to be incorporated into the apatite crystal lattice, it must be bound to the nanocrystals' surface where it stabilizes the nanocrystals' size by preventing their further growth. The findings were published in the Dec. 28, 2010 issue of the Proceedings of the National Academy of Sciences.

"Based on the old literature, we looked at the citrate levels in a variety of types of bone and found that herring spine had the highest citrate concentration about 13 percent by weight," Schmidt-Rohr said. "So it should hold that the citrate signal for herring spine should be three times higher than for cow bone, and indeed it was."

In further studies, the group found that higher concentration of citrate, the thinner the apatite nanocrystals in bone. This was further confirmed on bone-mimetic nanocomposites in a collaborat
'/>"/>

Contact: Kerry Gibson
kgibson@ameslab.gov
515-294-1405
DOE/Ames Laboratory
Source:Eurekalert  

Page: 1 2 3

Related biology news :

1. Bone-munching worms from the deep sea thrive on fish bones
2. Bones of long-dead animals conjure ghosts at Yellowstone
3. Prostate cancer spreads to bones by overtaking the home of blood stem cells
4. In the lab, engineers novel liquid provides a solid fix for broken bones
5. More than 1,000 tigers reduced to skin and bones in last decade
6. Daily vibration may help aging bones stay healthy
7. Research could change course of treatment for cancer that spreads to bones
8. Diabetes risk in children increases risk for weak bones
9. Ear bones reveal spawning secrets of Lake Erie walleye
10. Protein identified that can result in fragile bones
11. Vigorous exercise strengthens hip bones in young children
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Citrate key in bone's nanostructure
(Date:7/9/2014)... but often can be defeated with antibiotics and then ... Now, scientists have built a new weapon against such pathogens ... journal ACS Applied Materials & Interfaces , their study ... them than medicine alone. , David Leong, Jianping Xie and ... undetectable in the human body or in places that antibiotics ...
(Date:7/9/2014)... a sparrow-sized fossil from China challenges the commonly held ... gained the ability to fly. The birdlike fossil is ... rather the remains of a tiny tree-climbing animal that ... Dinosaur Museum in Blanding, Utah, and Alan Feduccia of ... Springer,s Journal of Ornithology . , The fossil ...
(Date:7/9/2014)... one of the amphibians with the highest distribution in ... of water where it comes into contact with the ... carried out by the Spaniard Germn Orizaola from the ... these frogs have developed a defensive response to the ... bodies if they co-exist with the crayfish. , Numerous ...
Breaking Biology News(10 mins):Researchers declassify dinosaurs as being the great-great-grandparents of birds 2Frogs have developed rapid defences against the red swamp crayfish 2Frogs have developed rapid defences against the red swamp crayfish 3
... machines may not always use hot enough water to ... Acinetobacter, a Gram-negative bacteria, from hospital uniforms, according to ... Infection Control and Hospital Epidemiology , the journal of ... The study, conducted by researchers from University College in ...
... an unprecedented depletion of the Earth,s protective ozone layer above ... an unusually prolonged period of extremely low temperatures in the ... of the international team behind the study to be published ... researchers found the amount of ozone destroyed in the Arctic ...
... Philadelphia -- Researchers have determined that a combination ... viral E6 can accurately determine the oropharyngeal squamous cell ... derive from HPV16, according to a study published in ... American Association for Cancer Research. "This has immediate ...
Cached Biology News:Study: Residential washers may not kill hospital-acquired bacteria 2Unprecedented Arctic ozone loss occurred last winter 2Unprecedented Arctic ozone loss occurred last winter 3
(Date:1/15/2014)... The Pittcon Organizing Committee is pleased to announce that Innovative ... e-Journal and producer of Food Labs Conference , ... the co-location of Food Labs Conference to be held in ... fee to attend the two-day Food Lab Conference, March 4-5. ...
(Date:1/15/2014)... Look inside the new Preferred Solutions® ... lab, from fluid handling to instruments to supplies. Many ... you order. , Preferred Solutions features a full ... L/S® model for precise flow control and dispensing to ...
(Date:1/15/2014)... and SAN JOSE, California , January ... company developing antibody-drug conjugates for cancer, today announced the appointment ... Directors. Dr Reynolds has over 20 years, development experience gained ... at Seattle Genetics. "I am delighted to welcome ...
(Date:1/15/2014)... 2014 This webinar will focus on ... safety assessment in biosimilars. , Regulatory frameworks are evolving ... development, however the complex nature of biopharmaceuticals makes the ... efficacy extremely challenging. Based on the specific aspects of ...
Breaking Biology Technology:Pittcon Announces Second Year Partnership With Food Safety Tech 2Pittcon Announces Second Year Partnership With Food Safety Tech 3Cole-Parmer Begins 2014 with the Release of Preferred Solutions 2Oxford BioTherapeutics Appoints Thomas C Reynolds MD, PhD to its Board of Directors 2Oxford BioTherapeutics Appoints Thomas C Reynolds MD, PhD to its Board of Directors 3Xtalks Life Sciences Webinar Examines Safety Assessment of Biosimilars 2
... announces that a new market research report is ... applications, markets and companies ... creation and utilization of materials, devices, and systems ... scale (a nanometer is one billionth of a ...
... strip with two surfaces but only one side, has been ... German mathematician August Mbius. As artist M.C. Escher so vividly ... traverse the "inside" and "outside" surfaces of a Mbius strip ... searching for an example of Mbius symmetry in natural materials ...
... Dec. 20, 2010 Metamark Genetics, Inc., ... and HistoRx, Inc., a diagnostics company developing ... care, today announced a multi-year licensing agreement. ... to the AQUA technology for the clinical ...
Cached Biology Technology:Reportlinker Adds Nanobiotechnologies- applications, markets and companies 2Reportlinker Adds Nanobiotechnologies- applications, markets and companies 3Reportlinker Adds Nanobiotechnologies- applications, markets and companies 4Reportlinker Adds Nanobiotechnologies- applications, markets and companies 5Reportlinker Adds Nanobiotechnologies- applications, markets and companies 6Reportlinker Adds Nanobiotechnologies- applications, markets and companies 7Reportlinker Adds Nanobiotechnologies- applications, markets and companies 8Reportlinker Adds Nanobiotechnologies- applications, markets and companies 9Reportlinker Adds Nanobiotechnologies- applications, markets and companies 10Reportlinker Adds Nanobiotechnologies- applications, markets and companies 11Reportlinker Adds Nanobiotechnologies- applications, markets and companies 12Reportlinker Adds Nanobiotechnologies- applications, markets and companies 13Reportlinker Adds Nanobiotechnologies- applications, markets and companies 14Reportlinker Adds Nanobiotechnologies- applications, markets and companies 15Reportlinker Adds Nanobiotechnologies- applications, markets and companies 16Reportlinker Adds Nanobiotechnologies- applications, markets and companies 17Reportlinker Adds Nanobiotechnologies- applications, markets and companies 18Reportlinker Adds Nanobiotechnologies- applications, markets and companies 19Reportlinker Adds Nanobiotechnologies- applications, markets and companies 20Reportlinker Adds Nanobiotechnologies- applications, markets and companies 21Strange new twist: Berkeley researchers discover Möbius symmetry in metamaterials 2Strange new twist: Berkeley researchers discover Möbius symmetry in metamaterials 3Strange new twist: Berkeley researchers discover Möbius symmetry in metamaterials 4Metamark and HistoRx Announce Licensing Agreement for AQUA® Technology 2
... sensitive reagent for the detection of inorganic ... of recombinant E. coli phosphate-binding protein labeled ... to changes in its environment.How it worksBinding ... rapid and tight (Kd ~ 0.1 M), ...
... Costar strip ejector is a unique product ... strips from their holder. Linkage: This CLS ... to easily match Cornings product number. If ... the old Sigma-Aldrich number (Z71,314-7) or contact ...
... hood ventilates and purifies hazardous chemical ... exhaust. Bonded charcoal filters remove most ... clearance (with access shield open) accommodates ... options include final HEPA filter, work ...
Sigmas E-TOXATE (Limulus Amebocyte Lysate) test kits are intended for the detection and semiquantitation,of endotoxins for research purposes....
Biology Products: