Navigation Links
Circuitry of cells involved in immunity, autoimmune diseases exposed
Date:3/6/2013

aning.

To do so, they needed a technology that would allow them to silence genes without perturbing the cells in the process. Although RNA interference (RNAi) is a powerful way to turn off individual genes, most RNAi techniques rely on viruses as delivery vehicles. When scientists tried to perturb the T cells using these traditional techniques, cells either changed or died, limiting the effectiveness of these strategies.

"This was a real challenge," said Kuchroo. "Every time we tried to downregulate a gene with existing technologies, the cell would change. We didn't know if we were looking at the right thing. We needed a new technology something that could have a dramatic but precise effect."

A solution came from an unlikely source. Harvard professor and Broad associate member Hongkun Park and his lab in the departments of chemistry and chemical biology and of physics had been working on a computer-chip-like structure to interact with brain cells. Co-first authors Alex Shalek and Jellert Gaublomme along with other lab members had developed a bed of silicon nanowires miniscule needles designed to pierce cells.

"We learned that we could use these needles to deliver molecules into cells in a minimally invasive fashion," said Park. "And as Vijay and Aviv taught me, there are lots of things that this allows you to do that you could not do before. It's been an eye-opening experience."

Just as the thin needle of a syringe can be inserted into the skin and cause no more than a small pinching sensation, nanowires can be inserted into cells, causing minimal disruption. Using this new technology, the team teased apart the network, piece by piece, by deleting each of the key genes required in the development of Th17 cells.

With the help of co-first author Nir Yosef, a postdoc at the Broad and Brigham and Women's Hospital, the team found that Th17 cells are governed by two networks, seemingly at odds with each other: one
'/>"/>

Contact: Haley Bridger
hbridger@broadinstitute.org
617-714-7968
Broad Institute of MIT and Harvard
Source:Eurekalert

Page: 1 2 3 4 5

Related biology news :

1. Brain circuitry is different for women with anorexia and obesity
2. 1 region, 2 functions: Brain cells multitasking key to understanding overall brain function
3. U of M researchers utilize genetically corrected stem cells to spark muscle regeneration
4. Brain adds cells in puberty to navigate adult world
5. Novel combination therapy shuts down escape route, killing glioblastoma tumor cells
6. Scientists find bone-marrow environment that helps produce infection-fighting T and B cells
7. Engineering cells for more efficient biofuel production
8. Bone marrow cells used in bladder regeneration
9. Why cells stick: Phenomenon extends longevity of bonds between cells
10. Long noncoding RNAs control development of fat cells
11. Research improving breast cancer treatment by targeting tumor initiating cells
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:10/16/2014)... the human body. Battles are won, lost or ... this stalemate—known as tumor dormancy—can last up to ... that is poorly understood. , A new ... Torquato, a Professor of Chemistry at Princeton University, ... and the switch to a malignant state. Published ...
(Date:10/16/2014)... are anything but sustainable: environmental damage to soil ... increasingly evident. Despite their disadvantages, however, monocultures remain ... the sole possibility of achieving higher yields in ... an ecology professor at the University of Zurich, ... forestry. After all, a new study carried out ...
(Date:10/15/2014)... WASHINGTON, N.Y. , Oct. 15, 2014 /PRNewswire/ ... technology solutions for home and community-based care, today ... benefits of implementing Sandata,s Santrax® Electronic Visit Verification™ ... Care Services is a home health company founded ... Texas . The ...
Breaking Biology News(10 mins):Modeling tumor dormancy 2Plant communities produce greater yield than monocultures 2Plant communities produce greater yield than monocultures 3Sandata Announces Case Study with Quality Care Services, Inc. 2
... in cities have a smaller carbon footprint than people who ... analysis than in the past, scientists at Aalto University in ... same in the city and in the rural areas. More ... change are dependent upon how much goods and services people ...
... 2011 Highlights: ... the air Radio was integrated easily with Army,s cellular ... video to smartphones Harris Corporation,s (NYSE: ... wideband communications network infrastructure for various mission scenarios at the ...
... After being hunted to local extinction more than a century ... southern right whales of mainland New Zealand are coming home. ... first time that whales from a small surviving population around ... New Zealand mainland. Before the onslaught of 19th century ...
Cached Biology News:City dwellers produce as much CO2 as countryside people do 2Harris Corporation's Highband Networking Radio Provides Mobile, Wideband Communications for Empire Challenge 2011 2Harris Corporation's Highband Networking Radio Provides Mobile, Wideband Communications for Empire Challenge 2011 3Fighting back from extinction, New Zealand right whale is returning home 2Fighting back from extinction, New Zealand right whale is returning home 3
(Date:10/22/2014)... Grace Century, a private equity consultancy ... the addition of Dr. Yousef “Josh” Siddiqui , ... Dr. Siddiqui will provide further healthcare expertise to its ... University College Medical School in the late ... 2001. With further certification as a General Practitioner (GP), ...
(Date:10/22/2014)... YORK , Oct. 22, 2014 Nuvilex, ... Federation, approximately 400 million people worldwide are living with ... million people by 2030.  The global market for diabetes ... 2012 approximately 330,000 people worldwide died from pancreatic cancer.  ... death due to cancer in the United ...
(Date:10/20/2014)... Convey Computer , the leader ... Iowa State University won first place in the ... HC-2ex, the team’s solution achieved the highest overall performance ... second place finisher. , Experts from all segments of ... long challenge, using a variety of design tools, hardware ...
(Date:10/20/2014)... , Oct. 20, 2014 PureTech ... company tackling big healthcare problems, announced today the ... round, with participation from Invesco Perpetual, a $120 ... used to drive PureTech,s existing pipeline forward and ... "PureTech has the scientific creativity to really go ...
Breaking Biology Technology:Dr. Yousef Siddiqui joins the Grace Century Advisory Team 2Nuvilex Brief Analyst Report: Thinking Outside the Box by BrokerBank Securities, Inc. 2Team from Iowa State Wins 2014 MemoCODE Design Contest Using Convey HC-2ex 2Team from Iowa State Wins 2014 MemoCODE Design Contest Using Convey HC-2ex 3PureTech Announces Expansion with Oversubscribed $55M Funding Round 2PureTech Announces Expansion with Oversubscribed $55M Funding Round 3PureTech Announces Expansion with Oversubscribed $55M Funding Round 4PureTech Announces Expansion with Oversubscribed $55M Funding Round 5PureTech Announces Expansion with Oversubscribed $55M Funding Round 6
... Researchers and forensic agencies that test samples or screen post ... suffer from a number of limitations: , They ... commonly available for novel sample matrices such as saliva as they are for urine or ... costly due to the high volume of reagents used. , ...
... Introduction , ... of studying human gene activity and thereby providing insight ... developed the Expression Array System, a complete system for ... (utilizing fully curated transcript data for array design), the ...
... Furazolidone, Furaltadone, Nitrofurazone and Nitrofurantoine belong to the ... treatment of gastrointestinal infections in cattle and poultry. Due ... for furazolidone) was prohibited in 1993 in the European ... 1995 in the European animal breeding (VO (EWG) No. ...
Cached Biology Technology:Simultaneous Screening of 23 Drugs of Abuse in Oral Fluid Using an LC/MS/MS Method 2Simultaneous Screening of 23 Drugs of Abuse in Oral Fluid Using an LC/MS/MS Method 3Simultaneous Screening of 23 Drugs of Abuse in Oral Fluid Using an LC/MS/MS Method 4 Genome Wide Profiling of Paired Cancerous and Normal Breast Tissues and Rapid Interpretation of Gene Expression Data 2 Genome Wide Profiling of Paired Cancerous and Normal Breast Tissues and Rapid Interpretation of Gene Expression Data 3 Genome Wide Profiling of Paired Cancerous and Normal Breast Tissues and Rapid Interpretation of Gene Expression Data 4 Genome Wide Profiling of Paired Cancerous and Normal Breast Tissues and Rapid Interpretation of Gene Expression Data 5 Genome Wide Profiling of Paired Cancerous and Normal Breast Tissues and Rapid Interpretation of Gene Expression Data 6 Genome Wide Profiling of Paired Cancerous and Normal Breast Tissues and Rapid Interpretation of Gene Expression Data 7Detection of nitrofurane metabolites in food with HPLC/MS/MS 2