Navigation Links
Children's Hospital researchers identify genetic mutation that may predict organ rejection
Date:9/15/2008

Using a novel combination of cutting-edge technologies to scan the human genome, researchers at Children's Hospital of Pittsburgh of UPMC have identified a genetic mutation that identifies transplant recipients who experience rejection.

Known as a single nucleotide polymorphism (SNP), the genetic mutation validates the effectiveness of the system the researchers developed to search the human genome, according to principal investigator Rakesh Sindhi, MD, director of Pediatric Transplant Research in the Hillman Center for Pediatric Transplantation at Children's Hospital. They studied DNA samples from 80 children who received liver transplants and their parents.

"To identify mutations that mark a disease, from the millions of known mutations in the human genome, one needs to study hundreds, even thousands of patients with that disease. As a result, large-scale scanning of known mutations has not been applied to rarer diseases, such as those that affect children. However, by combining multiple layers of genetic information, with information from the cell types and processes affected by these genes, we can now study less common diseases using smaller numbers of subjects," Dr. Sindhi said. "Such mutations are likely to become the basis of a genomic fingerprint, which will allow us to predict who will experience rejection beforehand, and to personalize antirejection medication. The novel combination of techniques used in this study is a major methodological advance toward developing personalized diagnostics for transplant recipients, which will improve outcomes and quality of life."

Results of the study are published in the September issue of Gastroenterology, the official publication of the American Gastroenterological Association.

The approach used by Dr. Sindhi and colleagues involves powerful new tools known as microarrays. Over 500,000 known mutations spanning the entire genome are evaluated in one type of microarray. The substructure of gene products known as ribonucleic acid, or RNA, is evaluated for all known genes in another type of microarray. The mutation associated with rejection was first identified by comparing known mutations in children who received liver transplantation or were given a particular antirejection regimen, with those from their biological parents. Therefore, this study also provides evidence for the inherited basis of rejection and rejection-free outcomes on a given anti-rejection regimen.

Studying antirejection medications is important because, while they make transplantation possible, they also can have side effects such as infections and cancers, some of which can be life-threatening.

"By establishing a genomic fingerprint for rejection, and applying personalized antirejection strategies before the transplant even occurs, we are hopeful we can reduce rejection rates and drug-induced side effects for children with liver transplants, from 50 percent to 20 percent or less," Dr. Sindhi said.


'/>"/>

Contact: Marc Lukasiak
marc.lukasiak@chp.edu
412-692-7919
Children's Hospital of Pittsburgh
Source:Eurekalert

Related biology news :

1. Rhode Island Hospital study finds link between obesity, type 2 diabetes and neurodegeneration
2. Childrens Memorial Hospital of Chicago receives a grant from BioMarin to support adult PKU outreach
3. Montreal Heart Institute and Mount Sinai Hospital researchers contribute to Crohns disease study
4. Childrens Hospital leads projects to develop nations first heart assist devices for young children
5. Clinical trial that may help patients breathe easier begins at Central DuPage Hospital
6. MRSA in hospital intensive care -- whats growing where?
7. Texas Hospital nations first to use large-scale cocoon strategy against whooping cough
8. Pennsylvania Hospital recognized for excellence in bariatric surgery
9. Childrens Hospital neurosurgeon receives grant
10. Pennsylvania Hospital surgeon receives grant to develop molecular cardiac surgery
11. Childrens Hospital studying drug with the potential to prevent/delay onset of type 1 diabetes
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/7/2016)... , June 7, 2016  Syngrafii Inc. and ... business relationship that includes integrating Syngrafii,s patented LongPen™ ... project. This collaboration will result in greater convenience ... credit union, while maintaining existing document workflow and ... ...
(Date:6/2/2016)... 2016 The Department of Transport Management ... 44 million US Dollar project, for the , ... Personalization, Enrolment, and IT Infrastructure , to ... and implementation of Identity Management Solutions. Numerous renowned international vendors ... Decatur was selected for the most compliant and ...
(Date:6/2/2016)... -- Perimeter Surveillance & Detection Systems, Biometrics ... Support & Other Service  The latest report ... analysis of the global Border Security market . ... $17.98 billion in 2016. Now: In November ... software and hardware technologies for advanced video surveillance. ...
Breaking Biology News(10 mins):
(Date:6/23/2016)... ... 2016 , ... UAS LifeSciences, one of the leading manufacturers ... Probiotics, into Target stores nationwide. The company, which has been manufacturing high quality ... list of well-respected retailers. This list includes such fine stores as Whole Foods, ...
(Date:6/23/2016)... ... June 23, 2016 , ... Charm Sciences, Inc. is ... has received AOAC Research Institute approval 061601. , “This is another AOAC-RI approval ... Bob Salter, Vice President of Regulatory and Industrial Affairs. “The Peel Plate methods ...
(Date:6/23/2016)... ... June 23, 2016 , ... STACS DNA Inc., the sample tracking software company, ... Crime Laboratory, has joined STACS DNA as a Field Application Specialist. , “I ... President and COO of STACS DNA. “In further expanding our capacity as a scientific ...
(Date:6/23/2016)... NEW YORK , June 23, 2016 ... the trading session at 4,833.32, down 0.22%; the Dow Jones ... the S&P 500 closed at 2,085.45, down 0.17%. Stock-Callers.com has ... INFI ), Nektar Therapeutics (NASDAQ: NKTR ... BIND Therapeutics Inc. (NASDAQ: BIND ). Learn more ...
Breaking Biology Technology: