Navigation Links
Chemists devise inexpensive, benchtop method for marking and selecting cells
Date:1/8/2013

tina tough, sugar-derived material found, for example, in crab shells, squid beaks and the cell walls of fungi. In addition to a main chitin-breaking domain, chitinases have another active structure, a "chitin binding domain" (ChBD). "It makes a super-strong bond with chitin," said Peng. In recent years, scientists have begun to use this high-affinity binding of ChBD and chitin as a marker system, typically for selecting ChBD-tagged proteins in a lab dish. The new method uses ChBD to mark and select cells.

A Powerful Tool

In the basic technique, a new gene can be added to cells within a larger DNA vector that also includes the genetic sequences for ChBD and GFP. The ChBD molecule will be produced in such a way that it ends up being held on the outer surface of its host cell's plasma membraneand the GFP molecule will sit just inside the membrane. The GFP serves as a visual beacon, while the ChBD serves as a handy gripping point for cell selection.

After exposing a culture of test cells to this experimental ChBD-containing vector, the scientists was able to see, via the GFP tags, which cells were expressing them, and was able to select them out easily, with high sensitivity, using magnetic beads coated with chitin. "This is a relatively easy benchtop method," Peng said. Importantly, these selected cells could produce progeny cells that seemed normal and healthy.

Because the ChBD marker, in the vector, is produced in a way that anchors it to a cell's membrane, it also can serve as a powerful tool for selecting just the membrane fraction of a sample of cellular material. Peng and his colleagues demonstrated this using chitin beads to quickly isolate a pure fraction of membrane material from ChBD-marked test cells.

Cellulase enzymes, which break down the ubiquitous plant compound cellulose, also have a high-affinity cellulase-binding domain, which can be employed in the same way as the ChBD.

The scientists exp
'/>"/>

Contact: Mika Ono
mikaono@scripps.edu
858-784-2052
Scripps Research Institute
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. UC Berkeley chemists installing first carbon dioxide sensor network in Oakland
2. UMass Amherst biochemists developing tools to stop plague and other bacterial threats
3. Chemists advance clear conductive thin films
4. Chemists develop reversible method of tagging proteins
5. Inexpensive, abundant starch fibers could lead to ouchless bandages
6. New 3-D stem cell culture method published in JoVE
7. New methods for better purification of wastewater
8. More effective method of imaging proteins
9. New method for estimating parameters may boost biological models
10. New paper by Notre Dame researchers describes method for cleaning up nuclear waste
11. Scientists discover new method of proton transfer
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:7/10/2014)... , July 7, 2014 According ... Research "Electronic Access Control Systems Market Global Forecast, Market ... the global Electronic Access Control systems market was valued ... to grow at a CAGR of 12.6% from 2014 ... 31,187.8 million in 2019. Browse the full ...
(Date:7/10/2014)... , July 9, 2014  Acuity Market Intelligence today released ... 2014 Edition" indicating that the global market for National Electronic ... 2013 to 2018. During this time, the number of National ... 3.5 billion. Asia , with its vast ... National eID cards issued, while Europe ...
(Date:7/10/2014)... belongs to the large, mainly tropical flowering plant family ... in the family rely on bees for their pollination. Only ... or vertebrates. The flowers of Axinaea appear in ... petals of the different species are pink, yellow, orange or ... the contrasting colours of their bulbous appendages. The pollination mechanism ...
Breaking Biology News(10 mins):Electronic Access Control Systems Market is Expected to Reach USD 31.2 Billion Globally in 2019: Transparency Market Research 2Electronic Access Control Systems Market is Expected to Reach USD 31.2 Billion Globally in 2019: Transparency Market Research 3Electronic Access Control Systems Market is Expected to Reach USD 31.2 Billion Globally in 2019: Transparency Market Research 4Electronic Access Control Systems Market is Expected to Reach USD 31.2 Billion Globally in 2019: Transparency Market Research 5National Electronic ID Programs Generate $54 Billion Between 2013 and 2018 with 3.5 Billion National eID Card Holders Worldwide 2Novel type of bird pollination mechanism discovered in South America 2
... MINNEAPOLIS, MN November 19, 2013 Data reported ... Ulm Project reinforce the positive influence of lifestyle factors ... heart disease and other health problems. Findings based on ... of obesity prevention and nutrition, specifically eating more fruits ...
... are located without much more than a sideways glance? Quite ... lead author of a study conducted at the Yerkes National ... Animal Cognition . The study finds that monkeys are ... Humans are able to locate, and even side-step, objects in ...
... Va. , Nov. 19, 2013   The Bensalem Township ... a new initiative in the field of DNA testing.  This ... will be the first of its kind in the country ... has its own local DNA database with over 6,000 DNA ...
Cached Biology News:Obesity and nutrition are keys to avoiding metabolic syndrome 2Monkeys can point to objects they do not report seeing 290 Minute DNA Results, A First Of Its Kind, Coming To Bensalem 2
(Date:7/11/2014)... ON , July 11, 2014 /CNW/ - Sunovion ... approved APTIOM TM (eslicarbazepine acetate) for use as ... seizures in patients with epilepsy who are not satisfactorily ... indicated for use in patients under 18 years of ... most common neurological disorders and according to Epilepsy Canada, ...
(Date:7/10/2014)... (PRWEB) July 10, 2014 Deep ... Century Medicine Forum on ‘Commercialising Longevity Research’ and ... entrepreneurs to the London Bioscience Innovation Center for ... Foundation and Aging Analytics Ltd. The meeting highlighted ... in translational research for age-related disease, as well ...
(Date:7/10/2014)... (PRWEB) July 10, 2014 OMICS Group’s ... techniques during 18-20 August, 2014 at Double Tree by ... the recent developments in Analytical & Bioanalytical research methods ... , Speaking on this occasion, Dr. Srinubabu Gedela, MD ... Acta conference is a remarkable one in bringing a ...
(Date:7/10/2014)...  Kainos Capital, a firm specializing in acquiring and ... has acquired the Slim-Fast brand from Unilever. Unilever will ... the transaction were not disclosed. Slim-Fast is ... markets ready-to-drink shakes, powders, bars and snacks to retail ... in the United Kingdom and ...
Breaking Biology Technology:Sunovion Pharmaceuticals Canada Inc. Announces Health Canada Approval of APTIOM(TM) (eslicarbazepine acetate) as a Once-Daily Adjunctive Treatment for Partial-Onset Seizures in Patients with Epilepsy 2Sunovion Pharmaceuticals Canada Inc. Announces Health Canada Approval of APTIOM(TM) (eslicarbazepine acetate) as a Once-Daily Adjunctive Treatment for Partial-Onset Seizures in Patients with Epilepsy 3Sunovion Pharmaceuticals Canada Inc. Announces Health Canada Approval of APTIOM(TM) (eslicarbazepine acetate) as a Once-Daily Adjunctive Treatment for Partial-Onset Seizures in Patients with Epilepsy 4DKV Sponsors Forum on Commercialising Longevity Research; Brings Together Scientists, Investors, Entrepreneurs and Celebrities 2DKV Sponsors Forum on Commercialising Longevity Research; Brings Together Scientists, Investors, Entrepreneurs and Celebrities 3DKV Sponsors Forum on Commercialising Longevity Research; Brings Together Scientists, Investors, Entrepreneurs and Celebrities 4DKV Sponsors Forum on Commercialising Longevity Research; Brings Together Scientists, Investors, Entrepreneurs and Celebrities 5DKV Sponsors Forum on Commercialising Longevity Research; Brings Together Scientists, Investors, Entrepreneurs and Celebrities 6A Critical Review of Analytical Techniques for Improved Living Standards 2A Critical Review of Analytical Techniques for Improved Living Standards 3A Critical Review of Analytical Techniques for Improved Living Standards 4Kainos Capital Acquires Slim-Fast From Unilever 2
... 29 BioSpecifics Technologies Corp. (Nasdaq: BSTC ), ... today that it was added to the Russell 3000 and ... markets on June 29, 2009. The Russell family of U.S. ... comprehensive representation of the investable U.S. equity market. , ...
... Inc. (Nasdaq: MITI ), a biopharmaceutical company ... inflammation and autoimmune diseases, today announced that it was ... the annual reconstitution of the Russell indexes, which occurred ... the Russell 3000 as an established benchmark for tracking ...
... manufacturing activities for ANX-530 NDA re-started , , SAN ... ANX ) today announced its plans for the remainder ... that closed earlier this month, ADVENTRX has re-started the final ... addition, the Company will continue to evaluate the data from ...
Cached Biology Technology:BioSpecifics Technologies Corp. Added to Russell 3000 and Russell 2000 Indexes 2Micromet Added to Russell 3000 Index 2Micromet Added to Russell 3000 Index 3Micromet Added to Russell 3000 Index 4ADVENTRX Pharmaceuticals Announces Plans for Remainder of 2009 2ADVENTRX Pharmaceuticals Announces Plans for Remainder of 2009 3
Biotin or fluorescent labels can be attached and the stoichiometry of protein labeling determined....
...
These macrocarriers are for the biolistic PDS-1000 He and Hepta systems. Supplied as 500 macrocarriers....
...
Biology Products: