Navigation Links
Centromeres cross over, a lot
Date:6/12/2008

Recombination at centromeres is higher than anywhere else on the chromosome, even though methyltransferases do their best to prevent it, say Jaco et al., as published in the June 16 issue of the Journal of Cell Biology.

Centromeric recombination has been hard to study because the DNA at centromeres is so repetitiveit's hard to see when a segment has switched chromatids. Jaco et al. have now addressed this challenge by using CO-FISH (chromosome orientation fluorescence in situ hybridization). After replication, the two new strands are digested away, leaving the two old strands. Because the strands are complementary in sequence, they can be tagged with strand-specific fluorescent probes. Using just one probe, only one chromatid would show a signal if no recombination had occurred.

Instead, the authors found that both chromatids fluoresced. And not just at one pointon average, the authors counted, centromeres had undergone 15 recombination events. This is about six times the rate of recombination as that seen for an equal length of telomeric DNA, and 175 times the rate for genomic DNA as a whole.

Telomeric recombination is inhibited by protein complexes called shelterins and by DNA methylation. The centromere has no shelterin, but it is methylated. Knockdown of DNA methyltransferases increased recombination at the centromere by about 50%, and decreased centromere length, possibly because of misalignment between repeated segments during recombination, a common problem with repetitive DNA. How methylation limits recombination, and why centromeres didn't lengthen as well as shorten, are unknown.

Their repetitive structure makes centromeres recombinogenic by nature, and the authors suggest that epigenetic regulation may ensure the continued stability of essential binding regions for proteins that link to the centromere.


'/>"/>

Contact: Emma Hill
ehill@rockefeller.edu
212-327-8011
Rockefeller University Press
Source:Eurekalert

Page: 1

Related biology news :

1. Energy Crossroads conference April 30 and May 1 at Stanford University
2. Scientists find a fingerprint of evolution across the human genome
3. Shilatifard Lab sheds light on molecular machinery required for translation of histone crosstalk
4. Gene study supports single main migration across Bering Strait
5. A mechanism to explain biological cross-talk between 24-hour body cycle and metabolism
6. Research sheds new light on how diseases jump across species
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/31/2016)... March 31, 2016  Genomics firm Nabsys has completed ... Barrett Bready , M.D., who returned to the ... original technical leadership team, including Chief Technology Officer, ... Development, Steve Nurnberg and Vice President of Software and ... company. Dr. Bready served as CEO of ...
(Date:3/23/2016)... , March 23, 2016 ... erhöhter Sicherheit Gesichts- und Stimmerkennung mit Passwörtern ... (NASDAQ: MESG ), ein führender ... das Unternehmen mit SpeechPro zusammenarbeitet, um erstmals ... Finanzdienstleistungsbranche, wird die Möglichkeit angeboten, im Rahmen ...
(Date:3/22/2016)... PROVO and SANDY, Utah ... Ontario (NSO), which operates the highest sample volume laboratory ... and Tute Genomics and UNIConnect, leaders in clinical sequencing ... announced the launch of a project to establish the ... panel. NSO has been contracted by ...
Breaking Biology News(10 mins):
(Date:4/27/2016)... Virgin Islands (PRWEB) , ... April 27, 2016 ... ... Company Ltd. (d/b/a Biohaven) is pleased to announce the appointment of John Tilton as ... was an Executive Director and one of the founding commercial leaders responsible for ...
(Date:4/27/2016)... (PRWEB) , ... April 27, 2016 , ... Global ... mutual endorsement of an Asia-Pacific Symposium as other research and development initiatives for potential ... Santiago officials and top Global Stem Cells Group executives began meeting to establish a ...
(Date:4/26/2016)... , ... April 26, 2016 , ... The European ... been selected as one of three finalists for the European Inventor Award 2016 in ... innovation prize will be announced at a ceremony in Lisbon on June 9th. , ...
(Date:4/26/2016)... ... April 26, 2016 , ... Seattle ... significant operating grant from 1Plus12 Corporation. The grant will be used to further ... outlined on the organization's website http://www.ivsci.org , In accounting the grant ...
Breaking Biology Technology: