Navigation Links
Cellular team players
Date:6/30/2014

This news release is available in German.

As in a successful football match, all actors in a cell must play in perfect coordination. A typical example for this kind of cooperation can be seen in the heat shock protein Hsp90, which controls the proper folding of other proteins. Together with a second molecule, the co-chaperone P23, it splits the energy source ATP to release the energy it needs to do its work.

However, while normal enzyme reactions often are easy to follow because the involved proteins alter their conformations clearly, the interaction between P23 and ATP involves significantly less conspicuous changes in state.

Using a sophisticated methodology, a team led by Professor Thorsten Hugel, head of the Department for Molecular Machines at the TU Muenchen and member of the Excellence Cluster Nanosystemns Initiative Munich (NIM), has now managed to observe this reaction in detail for the first time step for step in an Hsp90 molecule, P23 protein and ATP.

Live transmission of molecular processes

To this end, the team adapted the so-called FRET (Foerster resonance energy transfer) methodology to suit their requirements. The approach works by using a variety of fluorescent pigment molecules bonded to specific sites in the involved components. When these complexes are excited with light of a specific wavelength, the pigments start to fluoresce in a kind of chain reaction. The emitted fluorescent light reveals the precise distance between the marked sites, right down to the nanometer.

To determine exactly how the components Hsp90, P23 and ATP interact with each other, the biophysicists observed the positions and bonding sequences of the individual molecules over a span of several minutes. From the resulting data they could deduce even the smallest of changes, as well as the biological function of the overall complex.

Energy production as a team effort

Using this approach, the Munich researchers successfully demonstrated in detail that the P23 protein strengthens ATP bonding, thereby significantly increasing the amount of energy exploited. They also showed that the two substances bond with Hsp90 this effectively only as a team, thereby allowing ATP splitting to be used so successful.

"Without P23 the heat shock enzyme effectively runs on idle," explains Bjoern Hellenkamp their results. "When P23 joins the game, it is like shifting into gear. The energy is released and the reaction moves clearly in one direction. This is referred to as directionality."

In the near future the biophysicists want to investigate in detail how Hsp90 uses the exploited energy. The newly established methodology also allows them to investigate other multicomponent systems with mechanisms that have eluded study because of their minimal conformational alterations.


'/>"/>

Contact: Dr. Andreas Battenberg
battenberg@zv.tum.de
49-892-891-0510
Technische Universitaet Muenchen
Source:Eurekalert  

Related biology news :

1. Researchers discover Trojan Horse method of penetrating cellular walls without harm
2. Family of proteins plays key role in cellular pump dynamics
3. Scientists capture most detailed images yet of humans tiny cellular machines
4. Which came first, bi- or tricellular pollen? New research updates a classic debate
5. UNC researchers link aging to cellular interactions that occur across generations
6. Organization of cellular photosystems
7. Protein rescues stuck cellular factories
8. Surface characteristics influence cellular growth on semiconductor material
9. Hot on the trail of cellular metabolism
10. Finding a few foes among billions of cellular friends
11. New technology detect cellular memory
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Cellular team players
(Date:5/16/2017)... May 16, 2017  Veratad Technologies, LLC ( www.veratad.com ... age and identity verification solutions, announced today they will ... 2017, May 15 thru May 17, 2017, in ... International Trade Center. Identity impacts the ... in today,s quickly evolving digital world, defining identity is ...
(Date:5/6/2017)... SINGAPORE , May 5, 2017 ... has just announced a new breakthrough in biometric ... that exploits quantum mechanical properties to perform ... new smart semiconductor material created by Ram Group ... across finance, entertainment, transportation, supply chains and security. ...
(Date:4/19/2017)... New York , April 19, 2017 ... competitive, as its vendor landscape is marked by the ... the market is however held by five major players ... Safran. Together these companies accounted for nearly 61% of ... of the leading companies in the global military biometrics ...
Breaking Biology News(10 mins):
(Date:9/17/2017)... ... 17, 2017 , ... GeneOne Life Science, Inc. announces that ... Safety (KMFDS) for an Investigational New Drug application for a Phase I/IIa study ... The study in Korea represents the second clinical trial for GLS-5300. , ...
(Date:9/14/2017)... ... September 14, 2017 , ... DrugDev ... biotech at the third annual DrugDev Summit, November 7-8, 2017 in Philadelphia, PA. ... most progressive clinical research leaders for best practice case studies, keynote presentations, lively ...
(Date:9/14/2017)... ... September 14, 2017 , ... AxioMed is pleased to ... Dr. Steven Yang completed the procedure on a 35-year-old female patient suffering from ... disc at level L5-S1. The patient failed conservative treatments prior to undergoing surgery. ...
(Date:9/14/2017)... ... September 14, 2017 , ... Cambridge Semantics , the ... that its Anzo Smart Data Lake has been named a KMWorld Trend-Setting ... that help organizations succeed in surpassing their knowledge management goals. KMWorld searches for ...
Breaking Biology Technology: